Team:Ciencias-UNAM

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Revision as of 00:08, 12 August 2013

Home

HOME

PROJECT

HUMAN PRACTICES

TEAM

SAFETY

Overview

General Design

Wet Lab

Dry Lab

Conclusions

References

Bios

Sponsors

Overview

Journalism

Interviews

THE PROJECT

THE PEOPLE

PARTS

ACHIEVEMENTS

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Read the abstract

Meet the team

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-|[[Image:Ciencias-UNAM_logo.png|200px|right|frame]]
-Project description
-The human peptide LL-37 is a cationic antimicrobial peptide with activity against Gram-negative and Gram-positive bacteria. It has been shown to protect against ''Helicobacter pylori'' infection and its related gastritis, besides of inhibiting the growth of other pathogenic bacteria in the gastrointestinal tract such as ''Salmonella typhimurium'' and ''Escherichia coli''.
+</div>
-Most of the current synthetic expression systems for LL-37 depend on the construction of soluble fusion partners to avoid cytotoxic effects of the antimicrobial peptide in the ''E. coli'' host strain. However, these fusion systems require additional cleavage steps using enzymatic or chemical methods, which makes them impossible to express an active LL-37 peptide in vivo. In order to create a resistant host that can export LL-37 to the media, we intend to overexpress the ''E. coli'' acrAB and tolC operons, which activate the AcrAB-TolC efflux pump, a mechanism that has been related with resistance to this and similar antimicrobial peptides by expulsion.
-Our aim is to create a system in which ''E. coli'' expels LL-37 only when'' H. pylori o''r other pathogenic bacteria, such as'' S. typhimurium'', ''E. coli'' or'' V. cholerae'', are present. In order to do this we are using the LsrA promoter, which allows transcription in the presence of AI-2, a molecule produced by bacteria to communicate via quorum-sensing. ''E. coli'' also produces AI-2, so we designed an antisense RNA with specific secondary structure to inhibit the translation of LuxS, the enzyme responsible of the production of AI-2 in '''E. coli.''
-|[[Image:Ciencias-UNAM_team.png|right|frame|Your team picture]]
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-|-
-|
-|align="center"|[[Team:Ciencias-UNAM | Team Ciencias-UNAM]]
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-!align="center"|[[Team:Ciencias-UNAM|Home]]
-!align="center"|[[Team:Ciencias-UNAM/Team|Team]]
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-!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Ciencias-UNAM Official Team Profile]
-!align="center"|[[Team:Ciencias-UNAM/Project|Project]]
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-!align="center"|[[Team:Ciencias-UNAM/Parts|Parts Submitted to the Registry]]
-!align="center"|[[Team:Ciencias-UNAM/Modeling|Modeling]]
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-!align="center"|[[Team:Ciencias-UNAM/Notebook|Notebook]]
-!align="center"|[[Team:Ciencias-UNAM/Safety|Safety]]
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-!align="center"|[[Team:Ciencias-UNAM/Attributions|Attributions]]
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