"
Team:Glendale CC AZ/Protocols/RestrictionDigest
From 2013.igem.org
(Difference between revisions)
E.Soboslay (Talk | contribs) |
E.Soboslay (Talk | contribs) |
||
| Line 9: | Line 9: | ||
-Ice and bucket/container | -Ice and bucket/container | ||
| - | |||
-Part A (Purified DNA, > 16ng/ul) | -Part A (Purified DNA, > 16ng/ul) | ||
-Part B (Purified DNA, > 16ng/ul) | -Part B (Purified DNA, > 16ng/ul) | ||
| Line 16: | Line 15: | ||
-NEB Buffer 2, 3.1,or Ecor1 | -NEB Buffer 2, 3.1,or Ecor1 | ||
-BSA (Not needed with 3.1 buffer) | -BSA (Not needed with 3.1 buffer) | ||
| - | -Restriction Enzymes: EcoRI, SpeI, XbaI, PstI | + | -Restriction Enzymes: EcoRI, SpeI, XbaI, PstI |
-Thermal cycler | -Thermal cycler | ||
== Procedure == | == Procedure == | ||
Revision as of 19:59, 11 August 2013
Restriction Digest Protocol
Protocol can be found at: http://parts.igem.org/Help:Protocols/Restriction_Digest
Materials
-Ice and bucket/container -Part A (Purified DNA, > 16ng/ul) -Part B (Purified DNA, > 16ng/ul) -Linearized plasmid backbone (25ng/ul) -dH2O -NEB Buffer 2, 3.1,or Ecor1 -BSA (Not needed with 3.1 buffer) -Restriction Enzymes: EcoRI, SpeI, XbaI, PstI -Thermal cycler
