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Revision as of 19:59, 11 August 2013 (view source) E.Soboslay (Talk | contribs) ← Older edit		Revision as of 22:50, 11 August 2013 (view source) E.Soboslay (Talk | contribs) Newer edit →
Line 9:		Line 9:
	-Ice and bucket/container		-Ice and bucket/container
-	-Part A (Purified DNA, > 16ng/ul)	+	-Part A (Purified DNA, > 16ng/µl)
-	-Part B (Purified DNA, > 16ng/ul)	+	-Part B (Purified DNA, > 16ng/µl)
-	-Linearized plasmid backbone (25ng/ul)	+	-Linearized plasmid backbone (25ng/µl)
	-dH2O		-dH2O
	-NEB Buffer 2, 3.1,or Ecor1		-NEB Buffer 2, 3.1,or Ecor1

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Revision as of 22:50, 11 August 2013

Restriction Digest Protocol

Protocol can be found at: http://parts.igem.org/Help:Protocols/Restriction_Digest

Materials

  -Ice and bucket/container
  -Part A (Purified DNA, > 16ng/µl)
  -Part B (Purified DNA, > 16ng/µl)
  -Linearized plasmid backbone (25ng/µl)
  -dH2O
  -NEB Buffer 2, 3.1,or Ecor1
  -BSA (Not needed with 3.1 buffer)
  -Restriction Enzymes: EcoRI, SpeI, XbaI, PstI
  -Thermal cycler 

Procedure