Team:DTU-Denmark/Notebook/12 August 2013

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==Main purpose==
==Main purpose==
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# PCR reaction to amplify backbone pZA21 containing araBAD and RFP
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# PCR reaction in order to amplify backbone pZA21 containing araBAD and RFP
# PCR reaction in order to amplify Nir1 and Nir2 fragments
# PCR reaction in order to amplify Nir1 and Nir2 fragments
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==Procedure==
==Procedure==
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# PCR mix for backbone was made according to standard protocol.
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*Primers - 1an and 1b 
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* Template - "Ara" sample (plasmid isolation pZA21:araBAD:RFP).
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* Program was based on standard program with 65 C of annealing temperature and 4 min of extension time.
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* Samples names: 1, 2, 3 and N (negative)
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# PCR for Nir1 and Nir2
==Results==
==Results==

Revision as of 15:25, 12 August 2013

12 August 2013

Contents

lab 208


Main purpose


  1. PCR reaction in order to amplify backbone pZA21 containing araBAD and RFP
  2. PCR reaction in order to amplify Nir1 and Nir2 fragments

Who was in the lab


Henrike, Kristian, Gosia

Procedure


  1. PCR mix for backbone was made according to standard protocol.
  • Primers - 1an and 1b
  • Template - "Ara" sample (plasmid isolation pZA21:araBAD:RFP).
  • Program was based on standard program with 65 C of annealing temperature and 4 min of extension time.
  • Samples names: 1, 2, 3 and N (negative)
  1. PCR for Nir1 and Nir2

Results


Conclusion


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