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Team:Paris Bettencourt/Notebook/Drug Screening/Friday 19th July.html
From 2013.igem.org
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<li><FONT COLOR="#222222">sD014/sD015</FONT>=sD001+pD004+pD006 E.coli: ΔcysI, Δfpr, ΔydbK::zmFNR; Spectinomycin, zmSIR; Chloramphenicol | <li><FONT COLOR="#222222">sD014/sD015</FONT>=sD001+pD004+pD006 E.coli: ΔcysI, Δfpr, ΔydbK::zmFNR; Spectinomycin, zmSIR; Chloramphenicol | ||
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| + | <br><b>Glycerol Stocks were made of the above mentioned strains</b> | ||
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| + | Single colonies from agar plates were picked and used to inoculate 5ml LB broth overnight. <br> 750ml of overnight culture was added to 250ml of 60% glycerol in a cryotube.<br> Two sets of Glycerol stocks were used for each of sD001+pD004, sD001+sD004+sD005, sD001+sD004+sD006; <br>one set was frozen at -20ºC and the other set was frozen at -80ºC. | ||
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Revision as of 10:25, 13 August 2013
Drug Screening
ASDFDay Numbersuffix Month
TRANSFORMATIONS WORKED!!! cells were stocked and entered in catalog.
- sD012/sD013=sD001+pD004+pD005 E.coli:ΔcysI, Δfpr, ΔydbK:: soFD, zmSIR ;Chloramphenicol ,zmFNR; Spectinomycin- with all three genes
- sD016/sD017=sD001+pD004 E.coli:ΔcysI, Δfpr, ΔydbK:: ,zmFNR; Spectinomycin
- sD014/sD015=sD001+pD004+pD006 E.coli: ΔcysI, Δfpr, ΔydbK::zmFNR; Spectinomycin, zmSIR; Chloramphenicol
Glycerol Stocks were made of the above mentioned strains Single colonies from agar plates were picked and used to inoculate 5ml LB broth overnight.
750ml of overnight culture was added to 250ml of 60% glycerol in a cryotube.
Two sets of Glycerol stocks were used for each of sD001+pD004, sD001+sD004+sD005, sD001+sD004+sD006;
one set was frozen at -20ºC and the other set was frozen at -80ºC.
