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Team:DTU-Denmark/Notebook/13 August 2013
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| - | ===Gel of | + | ===Gel of SLP screening PCR products (yesterday)=== |
Each gel is representing one [[Team:DTU-Denmark/Notebook/12_August_2013#Screening_PCR_on_pZA21:araBAD:RFP_spl| row]]; from left to right and up to down is row A to H: | Each gel is representing one [[Team:DTU-Denmark/Notebook/12_August_2013#Screening_PCR_on_pZA21:araBAD:RFP_spl| row]]; from left to right and up to down is row A to H: | ||
Revision as of 14:37, 13 August 2013
13 August 2013
Contents |
lab 208
Main purpose
Who was in the lab
Kristian, Henrike, Gosia
Procedure
PCR (promoter library)
PCR to amplify USER fragments of the constitutive SPL, the constitutive reference promoter and the arabinose reference promoter.
Used 5% DMSO, 2 uL of mM MgCl2 and GC-buffer.
constitutive SPL
- template: pZA21::RFP
- primers: 52a, 52b1
constitutive reference
- template: pZA21::RFP
- primers: 52a, 52b2
arabinose reference
- template:
- primers: 51a,51b1
program
| temperature | time | cycles | |
|---|---|---|---|
| 98C | 2:00 | - | |
| 98C | 2:00 | - | |
| 98C | 2:00 | - | |
| 98C | 2:00 | - | |
| 98C | 2:00 | - |
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Conclusion
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