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17/07/13
From 2013.igem.org
(Difference between revisions)
AliceHaworth (Talk | contribs) |
AliceHaworth (Talk | contribs) (→Double digest) |
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*50ng of DNA | *50ng of DNA | ||
| - | *2ul of | + | *2ul of 3.1 buffer |
| + | *1ul of each restriction (XbaI and PstIin the 6 samples, EcoRI and PstI in the 6 samples, 12 samples in total) | ||
| + | *Water to make total volume to 20ul | ||
Revision as of 11:13, 14 August 2013
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Isolating RFP plasmid
- Add 1.5ml of overnight culture to epindorph
- Centrifuge for 2mins
- Remove supernatent
- Repeat
- Use thermo scientific mini kit
- Measure concentration using nanodrop
Double digest
- 50ng of DNA
- 2ul of 3.1 buffer
- 1ul of each restriction (XbaI and PstIin the 6 samples, EcoRI and PstI in the 6 samples, 12 samples in total)
- Water to make total volume to 20ul
