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Team:Groningen/Labwork/14 August 2013
From 2013.igem.org
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<h2>Mirjam</h2> | <h2>Mirjam</h2> | ||
Ligation reaction of ΔCheY and ΔDes (with help of Chaline) | Ligation reaction of ΔCheY and ΔDes (with help of Chaline) | ||
| - | Transformation to <i>B. subtilis</i> ΔDes transformed in ΔCheY and ΔCheY into ΔDes. | + | <br>Transformation to <i>B. subtilis</i> ΔDes transformed in ΔCheY and ΔCheY into ΔDes. |
| - | Inoculation of Pdes-CheY-GFP in LB medium with ampicillin (although strange colonies grew on the negative control plate). The colonies didn't grew in liquid LB medium. | + | <br>Inoculation of Pdes-CheY-GFP in LB medium with ampicillin (although strange colonies grew on the negative control plate). The colonies didn't grew in liquid LB medium. |
<h2>Sander</h2> | <h2>Sander</h2> | ||
Revision as of 16:53, 14 August 2013
Mirjam
Ligation reaction of ΔCheY and ΔDes (with help of Chaline)Transformation to B. subtilis ΔDes transformed in ΔCheY and ΔCheY into ΔDes.
Inoculation of Pdes-CheY-GFP in LB medium with ampicillin (although strange colonies grew on the negative control plate). The colonies didn't grew in liquid LB medium.
Sander
made 3 inoculations of the cheY knockout and 3 inoculations of wild type bacillus in 0,4% agar with LB medium to test motility.Chaline
Did a restriction on CheY down and Des up with EcoRI and heat inactivated the samples.Run the samples over gel and the bands are visible.
Measured the concentration of CheY down and Des up.
iGEM 2013 Groningen
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