|
|
| Line 46: |
Line 46: |
| | <br> | | <br> |
| | | | |
| - | <font size="4"> '''5/3/13''' </font>
| |
| | | | |
| - | - Performed agar test, focusing primarily on ×8
| |
| - |
| |
| - | : [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 T7 phage selection method test|5.3 T7 phage selection method test]]
| |
| - |
| |
| - | - Processed phage amplification
| |
| - |
| |
| - | : [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 Phage Amplification/Purification|5.3 T7 phage amplification/purification]]
| |
| - |
| |
| - | <br>
| |
| - |
| |
| - | <font size="4"> '''5/4/13''' </font>
| |
| - |
| |
| - | - Performed dilution series using stock 5.3 (-1 through -11)
| |
| - |
| |
| - | - Started two 5mL overnights of BL21
| |
| - |
| |
| - | <br>
| |
| - |
| |
| - | <font size="4"> '''5/5/13''' </font>
| |
| - |
| |
| - | - Spot test using stock 5.3 and its dilution series (from 5.4)
| |
| - |
| |
| - | : [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 T7 phage selection method test||5.3 T7 phage amplification/purification]]
| |
| - |
| |
| - | - Started liquid culture for purification team (at around noon)
| |
| - |
| |
| - | : 1mL of BL21 overnight + 4mL of LB + 100μL -2 phage (received stock)
| |
| - |
| |
| - | - Started 5.5 amplification from a plaque test
| |
| - |
| |
| - | : [[5.5 Amplification from a plaque test]]
| |
| - |
| |
| - | <br>
| |
| - |
| |
| - | <font size="4"> '''5/6/13''' </font>
| |
| - |
| |
| - | - Continued [[5.3 T7 phage amplification/purification]]
| |
| - |
| |
| - | - Performed liquid culture phage concentration test
| |
| - |
| |
| - | : [[5.6 T7+ Liquid Culture Phage Concentration Test]]
| |
| - |
| |
| - | <br>
| |
| - |
| |
| - | <font size="4"> '''5/7/13''' </font>
| |
| - |
| |
| - | - Started two 5mL of E coli BL21 overnight
| |
| - |
| |
| - | - Designed procedure for applying mutagen and selecting for T7
| |
| - |
| |
| - | <br>
| |
| - |
| |
| - | <font size="4"> '''5/8/13''' </font>
| |
| - |
| |
| - | - Went over procedure for applying mutagen and PCR with Dr. Grose
| |
| - |
| |
| - | - Performed spot tests under [[5.6 T7+ Liquid Culture Phage Concentration Test]]
| |
| - |
| |
| - | - Started two 5mL BL21 overnights
| |
| - |
| |
| - | - Learned how to create LB plates
| |
| - |
| |
| - | <br>
| |
| - |
| |
| - | <font size="4"> '''5/9/13''' </font>
| |
| - |
| |
| - | - Practiced with ×6 and ×8 top agar
| |
| - |
| |
| - | : [[5.9 T7 selection method test #2]]
| |
| - |
| |
| - | - Started [[5.9 T7+ Liquid Culture Phage Concentration Test #2]]
| |
| - |
| |
| - | - Discussed plans and schedule for next week.
| |
| | | | |
| | </font> | | </font> |
"
