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Team:UNITN-Trento/Notebook/Labposts/06/30
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(Difference between revisions)
(Created page with "{ "date" : "2013-06-17", "author" : "thomas", "title" : "Cloning of EFE in pSB1C3", "content" : "I began the week doing some minipreps (x5 of EFE and x5 of pSB1C3+RFP) follow...") |
Revision as of 07:42, 9 September 2013
{ "date" : "2013-06-17", "author" : "thomas", "title" : "Cloning of EFE in pSB1C3", "content" : "I began the week doing some minipreps (x5 of EFE and x5 of pSB1C3+RFP) following this protocol.
Quantification results
I then digested the linearized pSB1C3 (500 ng, kindly offered by Caterina), pSB1C3 + RFP (500 ng) and EFE (1000 ng) with EcorI and PstI following the 2A assembly protocol.After that, I proceeded with the ligation and transformation in NEB10B cells. ",
"tags" : "EFE"
}
| Sample | EFE | pSB1C3 |
|---|---|---|
| 1 | 253,8 ng/ul | 161,9 ng/ul |
| 1 | 243,5 ng/ul | 160,9 ng/ul |
| 3 | 261,4 ng/ul | 142,5 ng/ul |
| 4 | 218,0 ng/ul | 168,4 ng/ul |
| 5 | 299,1 ng/ul | sample lost |
