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Team:UNITN-Trento/Notebook/Labposts/08/32
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(Difference between revisions)
(Created page with "{ "date" : "2013-08-13", "author" : "viola-thomas", "title" : "Triple short ligation of Plambda promoter, EFE+terminators in pSB1C3", "content" : "<html>Today we added SAP to...") |
Latest revision as of 07:37, 9 September 2013
{ "date" : "2013-08-13", "author" : "viola-thomas", "title" : "Triple short ligation of Plambda promoter, EFE+terminators in pSB1C3", "content" : "Today we added SAP to psb1c3 and DpnI to S04617 and to EFE+B0015. We followed the short cloning protocol so we didn't purified the digestion products and, after the deactivation of the enzymes used , we proceeded with the ligation following the standard quantities. We stimate the same lenght for both the inserts (EFE and s04617).
Triple ligation
",
"tags" : "pFixK-cI-pLambda-EFE"
}
| CTRLµl | 1:1µl | 1:2µl | |
|---|---|---|---|
| EFE+B0015 | 0 | 4.53 | 9.06 |
| s04617 | 0 | 4.53 | 9.06 |
| pSB1C3 | 7.5 | 7.5 | 7.5 |
| Buffer | 3 | 3 | 3 |
| Ligase | 1 | 1 | 1 |
| Water | 18.5 | 9.44 | 0.38 |
