"

From 2013.igem.org

Revision as of 04:14, 21 August 2013 (view source) Ｙｔ (Talk | contribs) (→LB Culture Medium) ← Older edit		Revision as of 04:21, 21 August 2013 (view source) Ｙｔ (Talk | contribs) (→Ristriction Enzyme Proccessing) Newer edit →
Line 97:		Line 97:
	|-		|-
	|0.6||0||0||1||8.4||10		|0.6||0||0||1||8.4||10
		+	|}
		+	{|class="wikitable"
		+	|8/20 J23100-RBS-GFP-DT(334μg/ml)||EcoRI||SpeI||10x buffer H||MilliQ||total
		+	|-
		+	|3.0||1||1||3||22||30
		+	|-
		+	|0.3||0.2||0||1||8.5||10
		+	|-
		+	|0.3||0||0.2||1||8.5||10
		+	|-
		+	|0.3||0||0||1||8.7||10
	|}		|}
		+
	not written yet		not written yet

---

Revision as of 04:21, 21 August 2013

Home	Team	Official Team Profile	Project	Parts Submitted to the Registry	Modeling	Notebook	Material and method	Safety	Attributions

""m&m""

Aug 20

Miniprep

DNA	concentration[μg/μL]	260/280	260/230
8/18 Ptet -1	220	1.69	1.87
8/18 Ptet -2	210	1.69	1.71
8/18 RBS-tetR-DT -1	236	1.63	1.69
8/18 RBS-tetR-DT -2	182	1.65	1.98
8/18 J23100-RBS-GFP-DT -1	334	1.71	2.12
8/18 J23100-RBS-luxR-DT -1	440	1.67	1.60
8/18 J23100-RBS-luxR-DT -2	344	1.68	1.83
8/18 J23100-RBS-lacZα-DT -1	280	1.70	1.81
8/18 RBS-lysis3 -1	282	1.67	1.76
8/18 RBS-lysis3 -2	212	1.30	1.35

LB Culture Medium

volume - 200mL

name	amount
Bacto Trypton	2g
Bacto yeast extract	1g
Nacl	1g
Agar Powder	2g
Amp	40μl

1.Measuring reagents and put they in the Erlenmeyer flask
2.diluting in measuring cylinder to 200ml total
3.autoclaving
4.adding ampicirine 40ml

not written yet

Ristriction Enzyme Proccessing

8/20 Ptet -1(220μg/mL)	EcoRI	XbaI	10x BSA	10x buffer M	MilliQ	total
4.5	1	1	3	3	17.5	30
0.5	0.2	0	1	1	7.3	10
0.5	0	0.2	1	1	7.3	10
0.5	0	0	1	1	7.5	10

8/20 RBS-tetR-DT -2(182μg/mL)	EcoRI	SpeI	10x buffer H	MilliQ	total
5.5	1	1	3	19.5	30
0.6	0.2	0	1	8.2	10
0.6	0	0.2	1	8.2	10
0.6	0	0	1	8.4	10

8/20 J23100-RBS-GFP-DT(334μg/ml)	EcoRI	SpeI	10x buffer H	MilliQ	total
3.0	1	1	3	22	30
0.3	0.2	0	1	8.5	10
0.3	0	0.2	1	8.5	10
0.3	0	0	1	8.7	10

not written yet

Transformation

Electrophoresis