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Revision as of 08:11, 21 August 2013 (view source) Mitsuaki (Talk | contribs) (→template) ← Older edit		Revision as of 08:22, 21 August 2013 (view source) Mitsuaki (Talk | contribs) (→Transformation) Newer edit →
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	====Transformation====		====Transformation====
-	<!-- こっから -->
	<div class="experiment">		<div class="experiment">
-	<span class="author">じっけんしゃ</span>	+	<span class="author">by Nakamoto</span>
	{| class="wikitable"		{| class="wikitable"
-	!Name||Well||Sample||Competent Cells||Total||Plate	+	!Name||Sample||Competent Cells(XL10-gold)||Total||Plate
	|-		|-
-	|いでんし||ぱーつのうぇる||なんちゃら&micro;L||ごにょごにょ&micro;L||ほにゃらら&micro;L||せいげんこーそ	+	|tRNA-Spinach-tRNA||1&micro;L||10&micro;L||11&micro;L||8/19 LB+Amp
	|-		|-
-	|いかどうよう|| || || || ||	+	|tetR-aptamer 12_PC(076)||1&micro;L||10&micro;L||11&micro;L||8/19 LB+Amp
	|-		|-
-	| || || || || ||	+	|tetR-aptamer 12_1R(113)||1&micro;L||10&micro;L||11&micro;L||8/19 LB+Amp
		+	|-
		+	|tetR-aptamer 12_1M(105)||1&micro;L||10&micro;L||11&micro;L||8/19 LB+Amp
		+	|-
		+	|pT181 attenuator||1&micro;L||10&micro;L||11&micro;L||8/19 LB+Amp
		+	|-
		+	|Fusin1 attenuator||1&micro;L||10&micro;L||11&micro;L||8/19 LB+Amp
		+	|-
		+	|Fusion3m2 attenuator||1&micro;L||10&micro;L||11&micro;L||8/19 LB+Amp
		+	|-
		+	|pT181 antisense||1&micro;L||10&micro;L||11&micro;L||8/19 LB+Amp
		+	|-
		+	|Fusion1 antisense||1&micro;L||10&micro;L||11&micro;L||8/19 LB+Amp
		+	|-
		+	|Fusion6 antisense||1&micro;L||10&micro;L||11&micro;L||8/19 LB+Amp
	|}		|}
		+	incubate 37℃ overnight 20:34~
	</div>		</div>
-	<!-- ここまでをコピーしてね -->
	====Electrophoresis====		====Electrophoresis====

---

Revision as of 08:22, 21 August 2013

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""m&m""

Aug 20

Miniprep

by kojima

DNA	concentration[μg/μL]	260/280	260/230
8/18 Ptet -1	220	1.69	1.87
8/18 Ptet -2	210	1.69	1.71
8/18 RBS-tetR-DT -1	236	1.63	1.69
8/18 RBS-tetR-DT -2	182	1.65	1.98
8/18 J23100-RBS-GFP-DT -1	334	1.71	2.12
8/18 J23100-RBS-luxR-DT -1	440	1.67	1.60
8/18 J23100-RBS-luxR-DT -2	344	1.68	1.83
8/18 J23100-RBS-lacZα-DT -1	280	1.70	1.81
8/18 RBS-lysis3 -1	282	1.67	1.76
8/18 RBS-lysis3 -2	212	1.30	1.35

LB Culture Medium

by Okazaki
volume - 200mL

Bacto Trypton	2g
Bacto yeast extract	1g
Nacl	1g
Agar Powder	2g
Amp	40μl

1.Measuring reagents and put they in the Erlenmeyer flask
2.diluting in measuring cylinder to 200ml total
3.autoclaving
4.adding ampicirine 40ml

not written yet

Ristriction Enzyme Proccessing

by Kojima and Ashida

8/20 Ptet -1(220μg/mL)	EcoRI	XbaI	10x BSA	10x buffer M	MilliQ	total
4.5	1	1	3	3	17.5	30
0.5	0.2	0	1	1	7.3	10
0.5	0	0.2	1	1	7.3	10
0.5	0	0	1	1	7.5	10

8/20 RBS-tetR-DT -2(182μg/mL)	EcoRI	SpeI	10x buffer H	MilliQ	total
5.5	1	1	3	19.5	30
0.6	0.2	0	1	8.2	10
0.6	0	0.2	1	8.2	10
0.6	0	0	1	8.4	10

8/20 J23100-RBS-GFP-DT(334μg/ml)	EcoRI	SpeI	10x buffer H	MilliQ	total
3.0	1	1	3	22	30
0.3	0.2	0	1	8.5	10
0.3	0	0.2	1	8.5	10
0.3	0	0	1	8.7	10

8/20 J23100-RBS-luxR-DT -2(344μg/mL)	EcoRI	XbaI	BSA	10x buffer M	MilliQ	total
2.9	1	1	3	3	19.1	30
0.3	0.2	0	1	1	7.5	10
0.3	0	0.2	1	1	7.5	10
0.3	0	0	1	1	7.7	10

8/20 RBS-lysis3 -1(282μg/mL)	EcoRI	SpeI	10x buffer H	MilliQ	total
3.5	1	1	3	21.5	30
0.4	0.2	0	1	8.4	10
0.4	0	0.2	1	8.4	10
0.4	0	0	1	8.6	10

8/17 DT -1(188μg/mL)	EcoRI	XbaI	10x BSA	10x buffer M	MilliQ	total
5.3	1	1	3	3	16.7	30
0.3	0.2	0	1	1	7.5	10
0.3	0	0.2	1	1	7.5	10
0.3	0	0	1	1	7.7	10

incubate ~20:00

Transformation

Electrophoresis