Revision as of 08:45, 21 August 2013

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RNA Oscillator

じっけんしゃ

by kojima

DNA	concentration[μg/μL]	260/280	260/230
8/18 Ptet -1	220	1.69	1.87
8/18 Ptet -2	210	1.69	1.71
8/18 RBS-tetR-DT -1	236	1.63	1.69
8/18 RBS-tetR-DT -2	182	1.65	1.98
8/18 J23100-RBS-GFP-DT -1	334	1.71	2.12
8/18 J23100-RBS-luxR-DT -1	440	1.67	1.60
8/18 J23100-RBS-luxR-DT -2	344	1.68	1.83
8/18 J23100-RBS-lacZα-DT -1	280	1.70	1.81
8/18 RBS-lysis3 -1	282	1.67	1.76
8/18 RBS-lysis3 -2	212	1.30	1.35

by Okazaki

1.Measuring reagents and put they in the Erlenmeyer flask
2.diluting in measuring cylinder to 200ml total
3.autoclaving
4.adding ampicirine 40ml

not written yet

by Kojima and Ashida

8/20 Ptet -1(220μg/mL)	EcoRI	XbaI	10x BSA	10x buffer M	MilliQ	total
4.5	1	1	3	3	17.5	30
0.5	0.2	0	1	1	7.3	10
0.5	0	0.2	1	1	7.3	10
0.5	0	0	1	1	7.5	10

8/20 RBS-tetR-DT -2(182μg/mL)	EcoRI	SpeI	10x buffer H	MilliQ	total
5.5	1	1	3	19.5	30
0.6	0.2	0	1	8.2	10
0.6	0	0.2	1	8.2	10
0.6	0	0	1	8.4	10

8/20 J23100-RBS-GFP-DT(334μg/ml)	EcoRI	SpeI	10x buffer H	MilliQ	total
3.0	1	1	3	22	30
0.3	0.2	0	1	8.5	10
0.3	0	0.2	1	8.5	10
0.3	0	0	1	8.7	10

8/20 J23100-RBS-luxR-DT -2(344μg/mL)	EcoRI	XbaI	BSA	10x buffer M	MilliQ	total
2.9	1	1	3	3	19.1	30
0.3	0.2	0	1	1	7.5	10
0.3	0	0.2	1	1	7.5	10
0.3	0	0	1	1	7.7	10

8/20 RBS-lysis3 -1(282μg/mL)	EcoRI	SpeI	10x buffer H	MilliQ	total
3.5	1	1	3	21.5	30
0.4	0.2	0	1	8.4	10
0.4	0	0.2	1	8.4	10
0.4	0	0	1	8.6	10

8/17 DT -1(188μg/mL)	EcoRI	XbaI	10x BSA	10x buffer M	MilliQ	total
5.3	1	1	3	3	16.7	30
0.3	0.2	0	1	1	7.5	10
0.3	0	0.2	1	1	7.5	10
0.3	0	0	1	1	7.7	10

incubate ~20:00

by Nakamoto

Name	Sample	Competent Cells(XL10-gold)	Total	Plate
tRNA-Spinach-tRNA	1µL	10µL	11µL	8/19 LB+Amp
tetR-aptamer 12_PC(076)	1µL	10µL	11µL	8/19 LB+Amp
tetR-aptamer 12_1R(113)	1µL	10µL	11µL	8/19 LB+Amp
tetR-aptamer 12_1M(105)	1µL	10µL	11µL	8/19 LB+Amp
pT181 attenuator	1µL	10µL	11µL	8/19 LB+Amp
Fusin1 attenuator	1µL	10µL	11µL	8/19 LB+Amp
Fusion3m2 attenuator	1µL	10µL	11µL	8/19 LB+Amp
pT181 antisense	1µL	10µL	11µL	8/19 LB+Amp
Fusion1 antisense	1µL	10µL	11µL	8/19 LB+Amp
Fusion6 antisense	1µL	10µL	11µL	8/19 LB+Amp

incubate 37℃ overnight 20:34~

by Kojima

Lane	Sample	Enzyme1	Enzyme2
1	8/20 Ptet -1	EcoRI	XbaI
2	8/20 Ptet -1	EcoRI	-
3	8/20 Ptet -1	-	XbaI
4	8/20 Ptet -1	-	-
5	8/20 RBS-tetR-DT -2	EcoRI	SpeI
6	8/20 RBS-tetR-DT -2	EcoRI	-
7	8/20 RBS-tetR-DT -2	-	SpeI
8	8/20 RBS-tetR-DT -2	-	-

@@ Line 198: / Line 198: @@
 ====Electrophoresis====
+<div class="experiment">
+<span class="author">by Kojima</span>
+{| class="wikitable"
+!Lane||Sample||Enzyme1||Enzyme2
+|-
+|1||8/20 Ptet -1||EcoRI||XbaI
+|-
+|2||8/20 Ptet -1||EcoRI||-
+|-
+|3||8/20 Ptet -1||-||XbaI
+|-
+|4||8/20 Ptet -1||-||-
+|-
+|5||8/20 RBS-tetR-DT -2||EcoRI||SpeI
+|-
+|6||8/20 RBS-tetR-DT -2||EcoRI||-
+|-
+|7||8/20 RBS-tetR-DT -2||-||SpeI
+|-
+|8||8/20 RBS-tetR-DT -2||-||-
+|}
+</div>