Team:DTU-Denmark/Notebook/21 August 2013

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(USER ligation and transformation)
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=lab 208=
=lab 208=

Revision as of 21:28, 16 September 2013

21 August 2013

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Contents

lab 208


Main purpose


Who was in the lab


Kristian, Henrike

Procedure


USER ligation and transformation

  • with opened pZA21::ara and HAO, AMO, Cyc
  • also tried to insert Nir1 + Nir2 in pZA21 with the fitting ends

Results


Gels

  • 1 kb ladder
  • pZA21::RFP:araBAD linearized
  • pZA21::RFP:araBAD linearized (duplicate)
  • test PCR Sec2
  • test PCR Sec2 (duplicate)
  • screening sample A4
  • screening sample A5
  • screening sample A6
  • screening sample A7
  • screening sample A8
  • screening sample A9
  • screening sample A10
  • screening sample A11
  • screening sample A12
  • screening sample B1
  • screening sample B2
  • screening sample B3
  • screening sample B4
  • 1 kb ladder

2013-08-21 big screen.jpg

  • 1 kb ladder
  • screening sample B5
  • screening sample B6
  • screening sample B7
  • screening sample B8
  • screening sample B9
  • screening sample B10
  • screening sample B11
  • screening sample B12
  • screening sample C1
  • screening sample C2
  • screening sample C3
  • screening sample C4
  • screening sample C5
  • 1 kb ladder

2013-08-21 small screen.jpg

  • 1 kb ladder
  • screening sample C6
  • screening sample C7
  • screening sample C8
  • screening sample C9
  • screening sample C10
  • screening sample C11
  • screening sample C12

2013-08-21 small screen 2.jpg

Conclusion


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