Team:DTU-Denmark/Notebook/7 June 2013

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=lab 208=
=lab 208=
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Revision as of 08:12, 27 August 2013

07 June 2013

Contents

lab 208

Main purposes today

  • helloworld-project
  • prepare solutions
  • autoclaving
  • plate E.coli

Who was in the lab

Kristian

Procedure

  1. 1M CaCl_2 (200ml) weighed off 22.206 g CaCl_2
  2. 50% glycerol (500 ml)
  3. LB medium (liquid) (3 L);

->20 g of LB in every 1 L of distilled water. Autoclaved medium

->in 1 of these L, 14 g of agar were added for making LB solid medium

plating E.coli

  • Colonies of Top 10 E.coli were streaked on two plates
  • Colonies of Top 10 + pZE21 em GFP were streaked on kanamycin-Agar -> 10 uL kan on each plate (LB + 30 ug/mL kan)

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