Team:NCTU Formosa/project

Introduction

The main aim of our “E.coline” project is to generate isobutanol, a promising eco-fuel, in a productive and efficient way.To produce isobutanol, at first we use four pyruvate catalytic enzyme genes: alsS, ilvC, ilvD, kivD all. We then designed a temperature control system to allow E.coli to produce optimum isobutanol before being poisoned by isobutyaldehyde. According to our data(Figure 8), our temperature control system had been proven to work successfully. Furthermore, in order to produce isobutanol more efficiently, we combined zinc fingers and our enzymes together and put the fusion proteins in catalytic pathway order, thus the isobutanol conversion process can be accelerated. Besides, our cellulose test result has proven that in our project we are truly able to convert agricultural trash into the precious isobutanol.

[http://30cm.me/igemc See the page]

sRNA

(undetermined)

37°C RBS

http://fitisafeministissue.files.wordpress.com/2013/03/orange-juice.jpg

A

The main aim of our “E.coline” project is to generate isobutanol, a promising eco-fuel, in a productive and efficient way.To produce isobutanol, at first we use four pyruvate catalytic enzyme genes: alsS, ilvC, ilvD, kivD all. We then designed a temperature control system to allow E.coli to produce optimum isobutanol before being poisoned by isobutyaldehyde. According to our data(Figure 8), our temperature control system had been proven to work successfully. Furthermore, in order to produce isobutanol more efficiently, we combined zinc fingers and our enzymes together and put the fusion proteins in catalytic pathway order, thus the isobutanol conversion process can be accelerated. Besides, our cellulose test result has proven that in our project we are truly able to convert agricultural trash into the precious isobutanol.

B

Overall, we’ve completed our whole project of utilizing glucose to produce isobutanol and reached a production rate which is higher than recently published papers (0.8%=6.4g/L). Furthermore, we have also realized our dream of “Changing Trash into Gold” which is converting agricultural trash into isobutanol.

Light regulator

(undetermined)

Pathway Regulation

Our system is consisted of two independent parts: light induced part and dark induced part. Each part is capable of expression two different genes depending on the conditions in which the bacteria are grown; and therefore, a total of four different gene expression can be regulated.