Revision as of 09:21, 11 September 2013

Notebook : July 4

For regulator system:

estimated the size of segments for bands of electrophoresis by using Clone Manager.
Made interpretation for electrophoresis. The picture shows that the experimental result was coherent with our estimation. We constructed our first BrioBrick, BBa_K1155000 (fnr+plasmid PSB1C3).
stored 2 colonies who contain BioBrick BBa_K1155000
the plasmid DNA extraction was performed for BBa_K1155000.
The extract of plasmid DNA which contain BBa_K1155000 was digested by Not I, Mlu I, Hpa I, and were amplified by PCR.
Seeded the 4 additional broths (2 for amilCP, 2 for FNR) for plasmid extraction(mini prep).

For PCBs sensor system:

constructing

@@ Line 4: / Line 4: @@
 ===''Summary: ''===
+For regulator system:
-*calculated the size of segments for bands of electrophoresis by using Clone Manager.
+*estimated the size of segments for bands of electrophoresis by using Clone Manager.
-*Made interpretation for electrophoresis. The picture shows that the experimental result was coherent with our estimation. We ensure that we had our first BrioBrick, BBa_K1155000 (fnr+plasmid PSB1C3).
+*Made interpretation for electrophoresis. The picture shows that the experimental result was coherent with our estimation. We constructed our first BrioBrick, BBa_K1155000 (fnr+plasmid PSB1C3).
 *stored 2 colonies who contain BioBrick BBa_K1155000
-*As the Bi.oBrick BBa_k1155000 was confirmed, the plasmid DNA extraction was performed
+*the plasmid DNA extraction was performed for BBa_K1155000.
-*Received the bacterial strain: pseudomonas KE707.
 *The extract of plasmid DNA which contain BBa_K1155000 was digested by Not I, Mlu I, Hpa I, and were amplified by PCR.
-*Seeded the 4 additional broths (2 for amilCP, 2 for FNR) for plasmid extraction).
+*Seeded the 4 additional broths (2 for amilCP, 2 for FNR) for plasmid extraction(mini prep).
+For PCBs sensor system:
+*Received the bacterial strain: pseudomonas KE707.
 =='''Lab work'''==