Team:Glendale CC AZ/Team

From 2013.igem.org

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=Team Bio=
 
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Early 2013 Dr. Tuohy and Dr. Ziemba teamed up with Glendale Community College with hopes of forming an iGEM team for the 2013 collegiate division. After a rigorous application process, many candidates were selected, the schools first iGEM team had been selected. During the spring once a week, the team meet to discuss project ideas and reviewed techniques as well as the theory behind them. As of late spring, the team decided to work with Deinococcus radiodurans, as well as other species in the family. In late May, the team started working 4 days a week, and with the help of Dr. Tuohy and Dr. Ziemba, the team worked nonstop on trying to achieve the project goal.
 
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==='''Students'''===
 
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<gallery>
 
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Image:AdamGCC.jpg|Adam[[https://2013.igem.org/Team:Glendale_CC_AZ/Adam's Bio]]
 
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Image:EricaGCC.JPG|Erica[[https://2013.igem.org/Team:Glendale_CC_AZ/Erica's Bio]]
 
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Image:EstherJuarezGCC.jpg|Esther[[https://2013.igem.org/Team:Glendale_CC_AZ/Esther's Bio]]
 
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Image:SeanGCC.JPG|Sean[[https://2013.igem.org/Team:Glendale_CC_AZ/Sean's Bio]]
 
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Image:ChristinaGCC.jpg|Christina[[https://2013.igem.org/Team:Glendale_CC_AZ/Christina's Bio]]
 
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Image:TeresaGCC.jpg|Teresa[[https://2013.igem.org/Team:Glendale_CC_AZ/Teresa's Bio]]
 
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Image:FernGCC.jpg|Fern[[https://2013.igem.org/Team:Glendale_CC_AZ/Fern's Bio]]
 
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Image:AndrewGCC.jpg|Andrew[[https://2013.igem.org/Team:Glendale_CC_AZ/Andrew's Bio]]
 
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Image:Yanet_Cora_Kopnina_Headshot_(Mobile).jpg|Yanet[[https://2013.igem.org/Team:Glendale_CC_AZ/Yanet's Bio]]
 
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</gallery>
 
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==Advisors==
 
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<gallery>
 
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Image:Dr._Ziemba_GCC.JPG‎|Dr. Robert Ziemba
 
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Image:JTGCC.jpg|Dr. James Tuohy
 
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Image:|Advisor 3
 
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</gallery>
 
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PprM- Teresa
PprM- Teresa
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=Team Bio=
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Early 2013 Dr. Tuohy and Dr. Ziemba teamed up with Glendale Community College with hopes of forming an iGEM team for the 2013 collegiate division. After a rigorous application process, many candidates were selected, the schools first iGEM team had been selected. During the spring once a week, the team meet to discuss project ideas and reviewed techniques as well as the theory behind them. As of late spring, the team decided to work with Deinococcus radiodurans, as well as other species in the family. In late May, the team started working 4 days a week, and with the help of Dr. Tuohy and Dr. Ziemba, the team worked nonstop on trying to achieve the project goal.
 +
 +
 +
 +
==='''Students'''===
 +
 +
<gallery>
 +
Image:AdamGCC.jpg|Adam[[https://2013.igem.org/Team:Glendale_CC_AZ/Adam's Bio]]
 +
Image:EricaGCC.JPG|Erica[[https://2013.igem.org/Team:Glendale_CC_AZ/Erica's Bio]]
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Image:EstherJuarezGCC.jpg|Esther[[https://2013.igem.org/Team:Glendale_CC_AZ/Esther's Bio]]
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Image:SeanGCC.JPG|Sean[[https://2013.igem.org/Team:Glendale_CC_AZ/Sean's Bio]]
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Image:ChristinaGCC.jpg|Christina[[https://2013.igem.org/Team:Glendale_CC_AZ/Christina's Bio]]
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Image:TeresaGCC.jpg|Teresa[[https://2013.igem.org/Team:Glendale_CC_AZ/Teresa's Bio]]
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Image:FernGCC.jpg|Fern[[https://2013.igem.org/Team:Glendale_CC_AZ/Fern's Bio]]
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Image:AndrewGCC.jpg|Andrew[[https://2013.igem.org/Team:Glendale_CC_AZ/Andrew's Bio]]
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Image:Yanet_Cora_Kopnina_Headshot_(Mobile).jpg|Yanet[[https://2013.igem.org/Team:Glendale_CC_AZ/Yanet's Bio]]
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</gallery>
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==Advisors==
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<gallery>
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Image:Dr._Ziemba_GCC.JPG‎|Dr. Robert Ziemba
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Image:JTGCC.jpg|Dr. James Tuohy
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Image:|Advisor 3
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</gallery>

Revision as of 07:40, 29 August 2013


Previous Research

Deinococcus radiodurans

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vivamus magna. Cras in mi at felis aliquet congue. Ut a est eget ligula molestie gravida. Curabitur massa. Donec eleifend, libero at sagittis mollis, tellus est malesuada tellus, at luctus turpis elit sit amet quam. Vivamus pretium ornare est.

PprI

For the team’s Igem project, we wanted to increase E. coli’s desiccation resistance to help it survive in a desert environment. To do this, we planned to insert different genes from the Deinococcus species into the E. coli to increase its resistance to dessication. One of the genes our Igem group wanted to investigate was PprI. PprI is the transcriptional regulator involved in the expression of many of the DNA damage response proteins in Deinococcus radiodurans. In 2012, the Osaka team transformed E. coli with PprI to determine its effects on hydrogen peroxide resistance. Because of this, a PprI part from this team was in the Igem parts database. This allowed us to order their PprI part from the Igem registry and perform our experiments with it.

LeA

During our research for desiccation-resistance factors, we came across with the 2010 Valencia iGEM project. They demonstrated that late embryogenesis abundant proteins (LEA) from soy beans provided protection against extreme temperatures, when expressed in E. coli. Since high temperatures might lead to desiccation, we decided to explore LEA proteins in more detail. Previous studies have reported that LEA protein enhances the tolerance to various environmental stresses in organisms (Liu, Zheng, Zhang, Wang, & Li, 2010). They were first discovered in cottonseed and other plants vegetative tissues that were exposed to cold temperatures, drought, and high salinity (Liu et al., 2010).

LEA proteins are part of an extensive multi-gene family and are classified based on their sequences and expression patterns (Liu et al., 2010). Since Deinococcus was our organism of interest, we looked for studies that had reported homolog LEA proteins in this bacterium.

PprA

Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Vestibulum tortor quam, feugiat vitae, ultricies eget, tempor sit amet, ante. Donec eu libero sit amet quam egestas semper. facilisis luctus, metus

Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Vestibulum tortor quam, feugiat vitae, ultricies eget, tempor sit amet, ante. Donec eu libero sit amet quam egestas semper. facilisis luctus, metus

RecA

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vivamus magna. Cras in mi at felis aliquet congue. Ut a est eget ligula molestie gravida. Curabitur massa. Donec eleifend, libero at sagittis mollis, tellus est malesuada tellus, at luctus turpis elit sit amet quam. Vivamus pretium ornare est.

PprM

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vivamus magna. Cras in mi at felis aliquet congue. Ut a est eget ligula molestie gravida. Curabitur massa. Donec eleifend, libero at sagittis mollis, tellus est malesuada tellus, at luctus turpis elit sit amet quam. Vivamus pretium ornare est.

References

  • Liu, Y., Zheng, Y., Zhang, Y., Wang, W., & Li, R. (2010, May 1). Soybean PM2 protein (LEA3) confers the tolerance of Escherichia coli and stabilization of enzyme activity under diverse stresses. Current Microbiology, 60(5), 373-378. doi:10.1007/s00284-009-9552-2
  • MAKAROVA, K. S., ARAVIND, L., WOLF, Y. I., TATUSOV, R., MINTON, K. W., KOONIN, E. V., & DALY, M. J. (2001, March). Genome of the extremely radiation-resistant bacterium Deinococcus radiodurans viewed from the perspective of comparative genomics. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 65(1), 44-79. doi:10.1128/MMBR.65.1.44–79.2001

D. radiodurans - Esther

PprA- Sean

RecA- Teresa

PprM- Teresa



Team Bio

Early 2013 Dr. Tuohy and Dr. Ziemba teamed up with Glendale Community College with hopes of forming an iGEM team for the 2013 collegiate division. After a rigorous application process, many candidates were selected, the schools first iGEM team had been selected. During the spring once a week, the team meet to discuss project ideas and reviewed techniques as well as the theory behind them. As of late spring, the team decided to work with Deinococcus radiodurans, as well as other species in the family. In late May, the team started working 4 days a week, and with the help of Dr. Tuohy and Dr. Ziemba, the team worked nonstop on trying to achieve the project goal.


Students

Adam[Bio]

Erica[Bio]

Esther[Bio]

Sean[Bio]

Christina[Bio]

Teresa[Bio]

Fern[Bio]

Andrew[Bio]

Yanet[Bio]

Advisors

Dr. Robert Ziemba

Dr. James Tuohy

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