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Team:DTU-Denmark/Notebook/28 August 2013
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| Temperature range || 58C-78C || 50C-65C | | Temperature range || 58C-78C || 50C-65C | ||
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| - | | | + | | Elongation time per cycle || 1:30 min || 2 min |
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Each reaction was run in one of the 12 different annealing temperatures within the range indicated in the table. | Each reaction was run in one of the 12 different annealing temperatures within the range indicated in the table. | ||
| + | We followed the [[Team:DTU-Denmark/Methods/PCR#Generel_example_on_a_normal_PCR-program|standard PCR program]]. | ||
==Results== | ==Results== | ||
Revision as of 14:34, 29 August 2013
28 August 2013
Contents |
Lab 208
Main purpose
Who was in the lab
Henrike, Kristian, Julia
Procedure
A gradient PCR was made for pSB1C3 and pZA21::ara for Nir.
The master mix contains:
- CG buffer,
- X7 polymerase,
- 2% of DMSO (1uL of 100%DMSO) per reaction
- 1mM MgCl2 (1uL of 50mM MgCl2) per reaction
| 1 | 2 | |
|---|---|---|
| Template | pSB1C3 | pZA21::ara |
| Primers | 57a + 57b | 58a + 58b |
| Temperature range | 58C-78C | 50C-65C |
| Elongation time per cycle | 1:30 min | 2 min |
Each reaction was run in one of the 12 different annealing temperatures within the range indicated in the table. We followed the standard PCR program.
Results
Conclusion
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