Team:Groningen/Labwork/5 September 2013

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The plates (pSB1C3-S1-S5 and pSB1C3-S2-S5) show a lot of colonies.
The plates (pSB1C3-S1-S5 and pSB1C3-S2-S5) show a lot of colonies.
<br>ColonyPCR is performed on 4 colonies per plate (total of 8 colonies) beside one positive control (pSB1C3-S1). The primers used are VF2 and VR (annealing temperature 58&deg;C).
<br>ColonyPCR is performed on 4 colonies per plate (total of 8 colonies) beside one positive control (pSB1C3-S1). The primers used are VF2 and VR (annealing temperature 58&deg;C).
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<img src="https://static.igem.org/mediawiki/igem.org/0/06/ColonyPCR_pSB1C3-S1-5_pSB1C3-S2-5.jpg" width="80%" >
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<br>PCR was performed on S3 and S9 using primer pair S34910.
<br>PCR was performed on S3 and S9 using primer pair S34910.

Revision as of 13:33, 5 September 2013

Claudio

The plates (pSB1C3-S1-S5 and pSB1C3-S2-S5) show a lot of colonies.
ColonyPCR is performed on 4 colonies per plate (total of 8 colonies) beside one positive control (pSB1C3-S1). The primers used are VF2 and VR (annealing temperature 58°C).


PCR was performed on S3 and S9 using primer pair S34910.
PCR was performed on S6, S13 and S14 using primer pair S16.
PCR was performed on S16 using primer pair true-S16.
The annealing temperature tested for all of them was 58°C.