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Team:Washington/DPNL DIGEST
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Revision as of 07:43, 7 September 2013
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DpnI Digest:
For most PCR digests, add 1ul to the PCR product. Place in 37C for 5-15 minutes.
Other info here:
FastDigest DpnI | Thermo Scientific
1 μL of enzyme (1 FDU) cleaves 1 μg of pBR322 DNA (dam methylated) in 5 minutes at 37°C in 1X FastDigest Buffer.
PCR product ~30-200ng/ul
NEB DpnI:
100% in buffer 2
75% in Buffer 3
100% in buffer 1
100% in buffer 4 & cutsmart
openwetware.org:
DpnI - OpenWetWare
