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Team:BYU Provo/Notebook/LargePhage/Springexp/Period3/Dailylog
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Today we wanted to see if we can transfer our current experiment from using E. coli W3110 as the host to E. coli B as the host. We set up multiple experiments. | Today we wanted to see if we can transfer our current experiment from using E. coli W3110 as the host to E. coli B as the host. We set up multiple experiments. | ||
| - | 1. We spot tested the following phage samples on W3110 and B: T1, T2, T3, T4Do stock, T4 mutated stock, T4-UV one-plaque plate harvest, T4-UV-mutated one-plaque plate harvest. | + | <br>1. We spot tested the following phage samples on W3110 and B: T1, T2, T3, T4Do stock, T4 mutated stock, T4-UV one-plaque plate harvest, T4-UV-mutated one-plaque plate harvest. |
| - | 2. We did a dilution series on the T1 stock to see how concentrated it is. We will need to know the PFUs in order to grow a successful liquid culture and mass produce it for our large phage amplification procedure. | + | <br>2. We did a dilution series on the T1 stock to see how concentrated it is. We will need to know the PFUs in order to grow a successful liquid culture and mass produce it for our large phage amplification procedure. |
| - | 3. We also infected two samples of E. coli B, each with 10 uL of 10^-6 T4Do to observe plaque formation. | + | <br>3. We also infected two samples of E. coli B, each with 10 uL of 10^-6 T4Do to observe plaque formation. |
===5/31/13=== | ===5/31/13=== | ||
Revision as of 20:54, 5 June 2013
Contents |
5/27/13
5/29/13
Today we wanted to see if we can transfer our current experiment from using E. coli W3110 as the host to E. coli B as the host. We set up multiple experiments.
1. We spot tested the following phage samples on W3110 and B: T1, T2, T3, T4Do stock, T4 mutated stock, T4-UV one-plaque plate harvest, T4-UV-mutated one-plaque plate harvest.
2. We did a dilution series on the T1 stock to see how concentrated it is. We will need to know the PFUs in order to grow a successful liquid culture and mass produce it for our large phage amplification procedure.
3. We also infected two samples of E. coli B, each with 10 uL of 10^-6 T4Do to observe plaque formation.
5/31/13
I took out the plates from last times experiments.
The titer for T4 was unsuccessful on the E.Coli B, so we'll need to try that again.
Also, today Jade taught me how to set up the website- so I worked on that.
June
6/3/13
KS- I spent most of today getting the website in order and organized for future experiments to be entered in easily!
6/5/13
6/7/13
