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Team:UNITN-Trento/Notebook/Labposts/07/22
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(Created page with "{ "date" : "2013-07-25", "author" : "fabio", "title" : " the blue ligation: the never ending story !", "content" : "<html> today I miniprepped and screened (on a medium and a...") |
Revision as of 07:26, 9 September 2013
{
"date" : "2013-07-25",
"author" : "fabio",
"title" : " the blue ligation: the never ending story !",
"content" : " today I miniprepped and screened (on a medium and a large size gel) the 6 ligation inocula!
![](\"https://static.igem.org/mediawiki/2013/c/ce/Tn-2013Eee.jpg\")
As we can see from the gel the ligation seems not to be occurred!! I forgot to run the plasmid (016) alone to see a difference with the ligations though!! However I need to digest k592016 again to repeat another ligation with the blu promoter!!this time I digested 3 ug of the A sample from the first set!!", "tags" : " YF1_FixJ - FixK2" }
