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Team:UNITN-Trento/Notebook/Labposts/06/13
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(Created page with "{ "date" : "2013-06-12", "author" : "Gabriele", "title" : "Ligation and transformation", "content" : "<html>Ligation of SAMsynthetase and pSB1C3 with and without RFP, so I pr...") |
Revision as of 07:39, 9 September 2013
{
"date" : "2013-06-12",
"author" : "Gabriele",
"title" : "Ligation and transformation",
"content" : "Ligation of SAMsynthetase and pSB1C3 with and without RFP, so I prepared four samples:
1) pSB1C3 with RFP (Ctrl_1)
2) pSB1C3 with RFP + SAMsynthetase (1:2)
3) pSB1C3 without RFP (Ctrl_2)
4) pSB1C3 without RFP (1:2)
The samples were prepared and ligation was performed following the ligation protocol.
Ligation parameters
- Plasmid concentration: 27.8ng/µl
- Plasmid length: 2070bp
- Insert concentration: 13.2ng/µl
- Insert length: 1155bp
- Used plasmid: 250µl
- Volume of reaction: 35µl
- Buffer concentration: 10X
Transformation in NEB10β cells was performed following the transformation protocol.Plates in incubator ON at 37°C static, more than 16 hours.", "tags" : "SAMsynthetase" }
