Team:Groningen/Labwork/9 September 2013

From 2013.igem.org

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S13 and S14 were digested with XbaI and PstI and purified.
S13 and S14 were digested with XbaI and PstI and purified.
<br>pSB1C3-S1-S5 and pSB1C3-S2-S5 were digested with SpeI and PstI.
<br>pSB1C3-S1-S5 and pSB1C3-S2-S5 were digested with SpeI and PstI.
 +
<br>pSB1C3-S1-S5 and pSB1C3-S2-S5 were ligated to S13 and S14, respectively.
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<br>The ligation product was transformed in <i>E. Coli</i> DH5&alpha; and plated on LB + Cm.
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<br>
<br>S3 and S9 were digested with BamHI and PstI.
<br>S3 and S9 were digested with BamHI and PstI.
<br>S16 was digested with EcoRI and BamHI.
<br>S16 was digested with EcoRI and BamHI.
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<br>
+
<br>S16 was ligated to S3 and S9.
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<br>The ligation reaction was run on gel 0.8% to check the presence of product.
<br>
<br>

Revision as of 13:32, 9 September 2013

Claudio

S13 and S14 were digested with XbaI and PstI and purified.
pSB1C3-S1-S5 and pSB1C3-S2-S5 were digested with SpeI and PstI.
pSB1C3-S1-S5 and pSB1C3-S2-S5 were ligated to S13 and S14, respectively.
The ligation product was transformed in E. Coli DH5α and plated on LB + Cm.

S3 and S9 were digested with BamHI and PstI.
S16 was digested with EcoRI and BamHI.
S16 was ligated to S3 and S9.
The ligation reaction was run on gel 0.8% to check the presence of product.

Sebas