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Team:Tuebingen/Notebook/Journal
From 2013.igem.org
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| - | <p><b>Inverter</b>: The progestin receptors inhibit the promotor fet3 (Pfet3) | + | <p><b>Inverter</b>: The progestin receptors inhibit the promotor fet3 (Pfet3) when progestin is present in the cell's medium. In order to receive a positive feedback when progestin is detected we need to invert the receptor signal. Therefore we have combined Pfet3 with a repressor-gene that represses the promotor of our reporter. Once progestin binds to the receptor Pfet3 is inhibited thus the expression of the repressor stops - the reporter is transcribed.</p> |
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Revision as of 15:52, 9 September 2013
Receptor: For simplification we denote the membranous progestin receptors (mPR) of Danio rerio and Xenopus laevis as "Receptor". One important property of the progestin receptor is its abilitiy to inhibit the fet3 promotor when progestin in absent. Since the Receptor serves as a sensor for progestins it is a is a very crucial element in our measurement system.
Inverter: The progestin receptors inhibit the promotor fet3 (Pfet3) when progestin is present in the cell's medium. In order to receive a positive feedback when progestin is detected we need to invert the receptor signal. Therefore we have combined Pfet3 with a repressor-gene that represses the promotor of our reporter. Once progestin binds to the receptor Pfet3 is inhibited thus the expression of the repressor stops - the reporter is transcribed.
Reporter: Our aim is the construction of an easy to use measuring system. Therefore we decided to use reporters that deliver optical feedback which then can be quantified with an inexpensive photometer. Thus lacZ and luciferse serve as reporters for our measurement system.
