Team:Groningen/Labwork/10 September 2013

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<h2>Mirjam</h2>
<h2>Mirjam</h2>
Genomic DNA extraction for &Delta;CheY and &Delta;Des.  
Genomic DNA extraction for &Delta;CheY and &Delta;Des.  
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<h2>Claudio</h2>
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pSB1C3-S1-S5-S13 and pSB1C3-S2-S5-S14 plates showed colonies.
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<br>ColonyPCR on 5 colonies per plate was performed using VF2 and VR primers (annealing temperature 58&deg;C).
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<br>The samples were checked on agarose gel 0.8% beside the positive control (pSB1C3-S1-S5).
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Revision as of 09:12, 10 September 2013

Mirjam

Genomic DNA extraction for ΔCheY and ΔDes.

Claudio

pSB1C3-S1-S5-S13 and pSB1C3-S2-S5-S14 plates showed colonies.
ColonyPCR on 5 colonies per plate was performed using VF2 and VR primers (annealing temperature 58°C).
The samples were checked on agarose gel 0.8% beside the positive control (pSB1C3-S1-S5).