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Team:Paris Saclay/Notebook/August/19
From 2013.igem.org
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| - | + | Expected size : | |
| + | * BphR2 Part I : 178 kb | ||
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| + | | style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | ||
| + | We obtain a frangment at the roght size. We can purify it. | ||
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=====2 - Gel purification of BphR2 ===== | =====2 - Gel purification of BphR2 ===== | ||
Revision as of 22:33, 10 September 2013
Contents |
Notebook : August 19
Lab work
A - Aerobic/Anaerobic regulation system
Objective : to obtain ...
1 - Gel purification of the digestion of Bba_K1155003 and Bba_K1155007 by XBaI/PstI
Nadia
Protocol : Gel purification
Nanodrop :
- RBS-LacZ-Term : 59.6ng/µL
- RBS-AmilCP-Term : 21.3ng/µL
|
Concentrations are very low so we try to precipitate the DNA again. |
2 - EtOH precipitation of RBS-LacZ-Term and RBS-AmilCP-Term
Nadia, XiaoJing
Protocol : EtOH precipitation Protocole ou à rédiger ici ?????????
We used 20µL of DNA.
Nanodrop :
- RBS-LacZ-Term : 38.4ng/µL
- RBS-AmilCP-Term : 28.7ng/µL
|
CONCLUSION |
A - Aerobic/Anaerobic regulation system / B - PCB sensor system
Objective : to obtain ...
1 - Electrophoresis gel of BphR2 produce by PCR
Damir
|
|
Expected size :
- BphR2 Part I : 178 kb
|
We obtain a frangment at the roght size. We can purify it. |
2 - Gel purification of BphR2
Damir
Protocol : Gel purification
Nanodrop :
- BphR2 : 57.2ng/µL
CONCLUSION
Meeting with Evry
