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Notebook : August 12

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining ...

1 - Electrophoresis of PCR products : NarK, NarG, NirB

Abdou

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Well 1 : 5µL of NirB+1µL of 6X loading dye
Well 2 : 5µL of NirB+1µL of 6X loading dye LA MEME CHOSE ???????
Well 3 : 5µL of NarG+1µL of 6X loading dye
Well 4 : 5µL of NarG+1µL of 6X loading dye
Well 5 : 6µL DNA Ladder
Well 6 : 5µL of NarK+1µL of 6X loading dye NUMERO 4 ???????????????
Well 7 : 5µL of NarK+1µL of 6X loading dye
Well 8 : 5µL of NarK+1µL of 6X loading dye
Gel : 1%

Expected sizes :

NarK, NarG, NirB : ...

We lost all our PCR products. We will do PCR again using more concentrated DNA and oligos.

2 - PCR of NarK, NarG, NirB

Abdou, Xavier

Used quantities : cf PCR précédente !!!!!!!!!!!!! ... ADN non dilué

PCR program : cf PCR précédente !!!!!!!!! SCHEMA !!!!!!!!!!!!!!

3 - Electrophoresis of PCR products : NarK, NarG, NirB