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Team:DTU-Denmark/Notebook/11 September 2013
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==Procedure== | ==Procedure== | ||
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| + | ===Colony PCR to verify Nir in pZA21::ara-tight=== | ||
| + | Colony PCR with sequencing primers Pair 1: Nir_FW_4_Seq + Nir_RV_5_Seq . Pair 2: Nir_FW_13_Seq + Nir_RV_14_Seq. | ||
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| + | Re-run of yesterday's PCR with the following new annealing temperatures: pair 1: 67C, pair 2: 68C | ||
==Results== | ==Results== | ||
Revision as of 14:46, 11 September 2013
11 September
Contents |
lab 208
Main purpose
Who was in the lab
Procedure
Colony PCR to verify Nir in pZA21::ara-tight
Colony PCR with sequencing primers Pair 1: Nir_FW_4_Seq + Nir_RV_5_Seq . Pair 2: Nir_FW_13_Seq + Nir_RV_14_Seq.
Re-run of yesterday's PCR with the following new annealing temperatures: pair 1: 67C, pair 2: 68C
Results
Gels
- 1 kb
- colony 1, HAO
- colony 2, HAO
- colony 3, HAO
- colony 4, HAO
- colony 5, HAO
- colony 6, HAO
- Nir colony 7, primer pair 1
- Nir colony 7, primer pair 2
- Nir colony 8, primer pair 1
- Nir colony 8, primer pair 2
- 1 kb
Made new PCR with higher annealing temperature to obliterate unspecific binding.
- 1 kb
- Nir colony 1, primer pair 1
- Nir colony 1, primer pair 1
- Nir colony 1, primer pair 2
- Nir colony 1, primer pair 2
