Team:BYU Provo/Notebook/Phage Purification/Fallexp/Period1/Dailylog
From 2013.igem.org
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We ran a T7 purification today to see if the regular WT T7 bands at a new location with our new CsCl stock. | We ran a T7 purification today to see if the regular WT T7 bands at a new location with our new CsCl stock. | ||
- | :-[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.76CsClGradient| 9. | + | :-[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.76CsClGradient| 9.7 CsCl Gradient]] |
AB DL AC | AB DL AC | ||
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+ | <font size="4"> '''9/11/13''' </font> | ||
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+ | - We went ahead and ran another phage purification procedure with the new phage suspension buffer created in [Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.910XPhageSuspensionBuffer| 9.9 10X Phage Suspension Buffer Creation]] | ||
+ | : -[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.11CsClGradient| 9.11 CsCl Gradient]] | ||
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+ | AB DL AC | ||
</font> | </font> |
Revision as of 20:54, 11 September 2013
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9/4/13 - Went over the results of our T4 and T7 purification with the Large Phage and small phage group. - Updated wiki and discussed plans to have everything completed by the deadlines. AB DL AC
9/6/13 Continued updating wiki. AB DL AC
9/7/13 We ran a T7 purification today to see if the regular WT T7 bands at a new location with our new CsCl stock. AB DL AC
9/9/13 We decided that our problem T7 banding in our gradient could be due to faulty phage suspension buffer. We created a new 10X solution of it to do all further gradients. AB DL AC
9/11/13 - We went ahead and ran another phage purification procedure with the new phage suspension buffer created in [Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.910XPhageSuspensionBuffer| 9.9 10X Phage Suspension Buffer Creation]] AB DL AC
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