Template:Team:Uppsala/JS/notebook
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{ | { | ||
- | ds = '< | + | ds = '<h2>Sunday 2013-06-30</h2><p><strong>Name of participants:</strong> Magnus B, Alexander W, Nils A, Mikael Strandgren (MS), Anders Edlund (AE)<br><br>'+ |
'<h3>Ongoing constructs:</h3>75. pEL3A15 - CrtB<br>25. pSB1C3-CrtY<br>27. pEL3K16-red<br>'+ | '<h3>Ongoing constructs:</h3>75. pEL3A15 - CrtB<br>25. pSB1C3-CrtY<br>27. pEL3K16-red<br>'+ | ||
'51.pEL3C18-red<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>'+ | '51.pEL3C18-red<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>'+ | ||
- | '21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br><br><h3>Todays work</h3><br><h3>Transformation</h3>'+ | + | '21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>'+ |
- | '75. pEL3A15 - CrtB<br><br><h3>Digest</h3>27. pEL3K16-red<br>51.pEL3C18-red<br><br><h3>O/N Screening</h3>'+ | + | '42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>'+ |
+ | '65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>'+ | ||
+ | '70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>'+ | ||
+ | '74. pSB3K3-Cp44-B0032-BFP<br>77. pSB4C15<br><br>'+ | ||
+ | '<b>L. reuteri:</b><br>Lb1. CF48-pLR581<br>Lb2. 1063-pLUL631<br>Lb3. DSM 20016-pVs2<br>'+ | ||
+ | 'Lb4. 100-23-noplasmid<br>Lb6. DSM 20016-pLUL63TsA8<br>Lb7. DSM 20016-pGFP<br>Lb8. 100-23-pGT232<br>'+ | ||
+ | 'Lb12. DSM 20016-noplasmid<br>Lb14. DSM 20016-pLUL631<br><br><strong>L. plantarum:</strong><br>Lb5. 256-rifR-pAMβ1<br><br>'+ | ||
+ | '<b>L. lactis:</b><br>Lb13. MG1363-pJP059<br>Lb15. unknown-pGus<br>Lb16. MG1363-noplasmid<br><br>'+ | ||
+ | 'Lb10. 256-noplasmid<br>Lb11. 36E-noplasmid<br>Lb9. JH2-2 pAMβ1<br><br><h3>Todays work</h3><br><h3>Transformation</h3>'+ | ||
+ | '61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>'+ | ||
+ | '67. pSB1C3-Cp44<br>77. pSB4C15-red<br>'+ | ||
+ | '75. pEL3A15 - CrtB<br><br><h3>Digest</h3>27. pEL3K16-red<br>51.pEL3C18-red<br><br>'+ | ||
+ | '<h3>Ligation</h3>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>'+ | ||
+ | '66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>77. pSB4C15-red<br>We made a transformation with one sample of 77 from today, and one from yesterday to try to see if the problem yesterday laid with the transformation or the assembly.<br><br><h3>O/N Screening</h3>'+ | ||
'25. pSB1C3-CrtY<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>'+ | '25. pSB1C3-CrtY<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>'+ | ||
- | '21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br><br><h3>Results</h3>19. pSB4C5-CrtE~I /w RBS, Linker & ZF failed<br>'+ | + | '21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br><br><h3>Results</h3><br><b>Re-streak</b><br>'+ |
- | '20. pSB4C5-CrtE~Z /w RBS, Linker & ZF failed<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed<br>75. pEL3A15 - CrtB failed<br><br>'; | + | 'Lb13. Lc. lactis MG1363 pJP059: Good growth<br>Lb16. Lc. lactis MG1363 noplasmid: Good growth<br>Lb15. Lc. lactis MG1363-pGUS: Good growth<br><br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF failed<br>'+ |
+ | '20. pSB4C5-CrtE~Z /w RBS, Linker & ZF failed<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed<br>75. pEL3A15 - CrtB failed<br><br>'; | ||
} | } | ||
else if(id == 'd201371') | else if(id == 'd201371') |
Revision as of 19:50, 12 September 2013