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Haloalkanes are widely used commercially. The majorty of these compounds have been shown to be serious pollutants as they are toxic and quite persistent in the environment, such as a man-made industrial chemical 1,2,3-Trichloropropane (TCP) and an organic pesticideγ-Hexachlorocyclohexane (Lindane，γ-HCH). These halogenated compounds have been introduced into our environment as a consequence of industrial waste disposal and widespread open use in agriculture, which need to be removed to low levels from waste streams and during sanitation of polluted sites. For this reason, the biodegradation of halogenated compounds is of great importance. In the project, several key enzymes in the biodegradation pathways of the two compounds were co-expressed in E. coli. As putting

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Project Description

Haloalkanes are widely used commercially. The majorty of these compounds have been shown to be serious pollutants as they are toxic and quite persistent in the environment, such as a man-made industrial chemical 1,2,3-Trichloropropane (TCP) and an organic pesticideγ-Hexachlorocyclohexane (Lindane，γ-HCH). These halogenated compounds have been introduced into our environment as a consequence of industrial waste disposal and widespread open use in agriculture, which need to be removed to low levels from waste streams and during sanitation of polluted sites. For this reason, the biodegradation of halogenated compounds is of great importance. In the project, several key enzymes in the biodegradation pathways of the two compounds were co-expressed in E. coli. As putting

Project Description

the different selected enzymes together, it will have a ability to degrade more halogenated compounds besides the both. To construct the efficient co-expression system and achieve biodegradation of γ-HCH and TCP, we adopt 2A peptide and polycistronic co-expression. Foot and mouth disease virus 2A peptide has been widely used for co-expression of multiple genes in eukaryote systems. However, the use of the 2A peptide in prokaryotes is limited, and so far only one paper described that F2A can work in E. coli as well. To explore whether the 2A peptides can work in our co-expression system, several vectors were constructed by using all three 2A peptides, respectively.

Full Page Image Gallery

In this tutorial we are going to create a stunning full page gallery with scrollable thumbnails and a scrollable full screen preview. The idea is to have a thumbnails bar at the bottom of the page that scrolls automatically when the user moves the mouse. When a thumbnail is clicked, it moves to the center of the page and the full screen image is loaded in the background.

Custom Animation Banner

In todayos tutorial we will be creating a custom animation banner with jQuery. The idea is to have different elements in a banner that will animate step-wise in a custom way.

We will be using the jQuery Easing Plugin and the jQuery 2D Transform Plugin to create some nifty animations.

Full Page Image Gallery

In this tutorial we are going to create a stunning full page gallery with scrollable thumbnails and a scrollable full screen preview. The idea is to have a thumbnails bar at the bottom of the page that scrolls automatically when the user moves the mouse. When a thumbnail is clicked, it moves to the center of the page and the full screen image is loaded in the background.

Hover Slide Effect

Today we will create a neat effect with some images using jQuery. The main idea is to have an image area with several images that slide out when we hover over them, revealing other images. The sliding effect will be random, i.e. the images will slide to the top or bottom, left or right, fading out or not. When we click on any area, all areas will slide their images out.

Annotation Overlay Effect

Today we will create a simple overlay effect to display annotations in e.g. portfolio items of a web designers portfolio. We got the idea from the wonderful portfolio of www.rareview.com where Flash is used to create the effect. We will use jQuery.