Team:Groningen/Labwork/13 September 2013

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(Difference between revisions)

Revision as of 13:05, 14 September 2013

Claudio

The plates pSB1C3-S16-3-5 and pSB1C3-S16-9-11 showed around thousands of colonies.
Colony PCR was performed (NEB Mater Mix) on 4 colonies per plate using VF2 and VR primers (annealing temperature 64°C).

Colony C from pSB1C3-S16-3-5 and colony D from pSB1C3-S16-9-11 were positive candidate and were inoculated in LB + Cm.

Plates (work previously done by Mirjam) containing several combination of signal sequences together with S3 or S9 showed colonies.
Colony PCR was performed (NEB Mater Mix) on 2 colonies per plate using VF2 and VR primers (annealing temperature 64°C).

Colony 1A, 2A, 5A, 7A-B and 8A showed to be positive candidates and were inoculated in LB + Cm.

Inne

Ran 2 gels with colony pcr samples.
Gel 1:

Ladder

s16-3-5 A

s16-3-5 B

s16-3-5 C

s16-3-5 D

s16-9-11 A

s16-9-11 B

s16-9-11 C

s16-9-11 D

Positive control

Gel 2

Ladder	1 A	1 B	empty	empty	2 A	2 B	3 A	3 B	4 A	4 B



Ladder	5 A	5 B	6 A	6 B	7 A	7 B	8 A	8 B	empty	empty

@@ Line 27: / Line 27: @@
 <br>Colony C from pSB1C3-S16-3-5 and colony D from pSB1C3-S16-9-11 were positive candidate and were inoculated in LB + Cm.
 <br>
-<br>Plates containing several combination of signal sequences together with S3 or S9 showed colonies.
+<br>Plates (work previously done by <b>Mirjam</b>) containing several combination of signal sequences together with S3 or S9 showed colonies.
 <br>Colony PCR was performed (NEB Mater Mix) on 2 colonies per plate using VF2 and VR primers (annealing temperature 64&deg;C).
 <br><img src="https://static.igem.org/mediawiki/2013/4/4d/ColonyPCR-SignalSeq_1.jpg" width="50%" >
 <br><img src="https://static.igem.org/mediawiki/2013/b/b4/ColonyPCR-SignalSeq_2.jpg" width="50%" >
+<br>Colony 1A, 2A, 5A, 7A-B and 8A showed to be positive candidates and were inoculated in LB + Cm.
 <h2>Inne</h2>