Team:Groningen/Labwork/14 September 2013

From 2013.igem.org

(Difference between revisions)
(Created page with "<html> <head> <meta http-equiv="X-UA-Compatible" content="IE=EDGE" charset="utf-8" /> <link rel="stylesheet" href="https://2013.igem.org/Team:Groningen/CSS/DefaultPage?action=ra...")
Line 27: Line 27:
<img src="" width="50%">
<img src="" width="50%">
<br>
<br>
-
<br>Plates containing pSB1C3-S1-S5-S13 and pSB1C3-S2-S5-S14 showed around 20 colonies.
+
<br>Plates containing pSB1C3-S1-S5-S13 and pSB1C3-S2-S5-S14 showed thousands of colonies.
 +
<br>Colony PCR (NEB Master Mix) was performed on 4 colonies per plate using VF2 and VR primers (annealing temperature 65&deg;C).
 +
<br>The samples were checked on agarose gel 0.8%.
 +
<img src="" width="50%">
<br>
<br>
-
 
+
<br>S13 and S14 were ligated into pSB1C3-S1-S5-S5 and pSB1C3-S2-S5-S5, respectively.
 +
<br>S13 and S14 were ligated into pSB1C3-S16-S3-S5 and pSB1C3-S16-S9-S11, respectively.
 +
<br>The ligation products were plated on LB + Cm.
</div>
</div>

Revision as of 14:20, 14 September 2013

Claudio

Plates containing BBa_K1085014-S1-S5-S14 and BBa_K1085014-S2-S5-S13 showed around 20 colonies.
Colony PCR (NEB Master Mix) was performed on 4 colonies per plate using F_spank_NheI and HM14 primers (annealing temperature 65°C).
The samples were checked on agarose gel 0.8%.

Plates containing pSB1C3-S1-S5-S13 and pSB1C3-S2-S5-S14 showed thousands of colonies.
Colony PCR (NEB Master Mix) was performed on 4 colonies per plate using VF2 and VR primers (annealing temperature 65°C).
The samples were checked on agarose gel 0.8%.

S13 and S14 were ligated into pSB1C3-S1-S5-S5 and pSB1C3-S2-S5-S5, respectively.
S13 and S14 were ligated into pSB1C3-S16-S3-S5 and pSB1C3-S16-S9-S11, respectively.
The ligation products were plated on LB + Cm.