Team:Groningen/Labwork/15 September 2013

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<br>Colony PCR (NEB Master Mix) was performed on 4 colonies per plate using VF2 and VR primers (annealing temperature 65&deg;C).
<br>Colony PCR (NEB Master Mix) was performed on 4 colonies per plate using VF2 and VR primers (annealing temperature 65&deg;C).
<br>The samples were checked on agarose gel 0.8%.
<br>The samples were checked on agarose gel 0.8%.
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<br>The gel didn't run well, however, one colony per plate was inoculated in LB + Cm for further screening.
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<br>Colony ?? from ?? and colony ?? from ?? show to be positive candidates and were inoculated in LB + Cm.
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<br>Colony PCR (NEB Master Mix) was performed on 4 colonies from plate BBa_K1085014-S1-S5-S14 using F_spank_NheI and HM14 primers (annealing temperature 68&deg;C).
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<br>The samples were checked on agarose gel 0.8%.
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<br>No positive colonies were screened (try again you will be more lucky! ;)).
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Revision as of 14:18, 15 September 2013

Claudio

Plates containing pSB1C3-S1-S5-S5-S13 and pSB1C3-S2-S5-S5-S14 showed thousands of colonies.
Colony PCR (NEB Master Mix) was performed on 4 colonies per plate using VF2 and VR primers (annealing temperature 65°C).
The samples were checked on agarose gel 0.8%.

Colony C from pSB1C3-S1-S5-S5-S13 and colony A from pSB1C3-S2-S5-S5-S14 showed to be positive candidates and were inoculated in LB + Cm.

Plates containing pSB1C3-S16-S3-S5-S13 and pSB1C3-S16-S9-S11-S14 showed thousands of colonies.
Colony PCR (NEB Master Mix) was performed on 4 colonies per plate using VF2 and VR primers (annealing temperature 65°C).
The samples were checked on agarose gel 0.8%.
The gel didn't run well, however, one colony per plate was inoculated in LB + Cm for further screening.