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Team:Calgary/Sandbox/Notebook/Protocols/RehydrationOfRegistryDNA
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Revision as of 04:50, 16 September 2013
Rehydration of Registry DNA
Protocol
- Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used.
- Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want;
- Add 10 μL of ddH2O (deionized water);
- Let the water incubate at room temperature for 10 minutes;
- Take 1 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies.
