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Team:DTU-Denmark/Notebook/7 June 2013
From 2013.igem.org
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*Colonies of Top 10 ''E.coli'' were streaked on two plates | *Colonies of Top 10 ''E.coli'' were streaked on two plates | ||
*Colonies of Top 10 + pZE21 em GFP were streaked on kanamycin-Agar -> 10 uL kan on each plate (LB + 30 ug/mL kan) | *Colonies of Top 10 + pZE21 em GFP were streaked on kanamycin-Agar -> 10 uL kan on each plate (LB + 30 ug/mL kan) | ||
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Navigate to the [[Team:DTU-Denmark/Notebook/5_June_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/9_June_2013|Next]] Entry | Navigate to the [[Team:DTU-Denmark/Notebook/5_June_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/9_June_2013|Next]] Entry | ||
{{:Team:DTU-Denmark/Templates/EndPage}} | {{:Team:DTU-Denmark/Templates/EndPage}} | ||
Revision as of 20:32, 16 September 2013
07 June 2013
Contents |
lab 208
Main purposes today
- helloworld-project
- prepare solutions
- autoclaving
- plate E.coli
Who was in the lab
Kristian
Procedure
- 1M CaCl_2 (200ml) weighed off 22.206 g CaCl_2
- 50% glycerol (500 ml)
- LB medium (liquid) (3 L);
->20 g of LB in every 1 L of distilled water. Autoclaved medium
->in 1 of these L, 14 g of agar were added for making LB solid medium
plating E.coli
- Colonies of Top 10 E.coli were streaked on two plates
- Colonies of Top 10 + pZE21 em GFP were streaked on kanamycin-Agar -> 10 uL kan on each plate (LB + 30 ug/mL kan)
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