"
Team:DTU-Denmark/Notebook/11 June 2013
From 2013.igem.org
(Difference between revisions)
| Line 48: | Line 48: | ||
|PCR tube total || ||50ul | |PCR tube total || ||50ul | ||
|} | |} | ||
| - | + | ||
Navigate to the [[Team:DTU-Denmark/Notebook/10_June_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/12_June_2013|Next]] Entry | Navigate to the [[Team:DTU-Denmark/Notebook/10_June_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/12_June_2013|Next]] Entry | ||
{{:Team:DTU-Denmark/Templates/EndPage}} | {{:Team:DTU-Denmark/Templates/EndPage}} | ||
Latest revision as of 20:59, 16 September 2013
11 June 2013
Contents |
208 lab
Main purposes today
helloworld-project
- diluting primers with 1.5 ml milli Q water each
- diluting G-block with 20ul milli Q water
Who was in the lab
Procedure
- 7 rxns in duplicates
- mastermix for 15 rxns
| per rxn | master | |
|---|---|---|
| 5x HF buffer | 10ul | 150ul |
| MgCl_2 | 0.5ul | 7.5ul |
| dNTPs | 5ul | 75ul |
| Phusion polymerase | 0.5ul | 7.5ul |
| Milli Q water | 25ul | 375ul |
| master mix total | 615 ul |
Every PCR tube contains
| mastermix | 41ul | |
| FW primer | 3ul | |
| RV primer | 3ul | |
| DNA template | 1ul | |
| PCR tube total | 50ul |
Navigate to the Previous or the Next Entry
