From 2013.igem.org
(Difference between revisions)
Revision as of 22:46, 16 September 2013
Aug 22
Liquid culture
Nakamoto
| Sample | Medium
|
| 8/21 Pcon-RBS-GFP-DT-Pcon-RBS-luxR-DT (1) | 8/21 Plusgrow medium (+Amp)
|
| 8/21 Pcon-RBS-GFP-DT-Pcon-RBS-luxR-DT (2) | 8/21 Plusgrow medium (+Amp)
|
| 8/21 Pcon-RBS-GFP-DT control (1) | 8/21 Plusgrow medium (+Amp)
|
| 8/21 Pcon-RBS-GFP-DT control (2) | 8/21 Plusgrow medium (+Amp)
|
| 8/21 RBS-lysis1 (1) | 8/21 Plusgrow medium (+Amp)
|
| 8/21 RBS-lysis1 (2) | 8/21 Plusgrow medium (+Amp)
|
| 8/21 RBS control (1) | 8/21 Plusgrow medium (+Amp)
|
| 8/21 RBS control (2) | 8/21 Plusgrow medium (+Amp)
|
| 8/21 Ptet(pm)-RBS-tetR-DT (1) | 8/21 Plusgrow medium (+Amp)
|
| 8/21 Ptet(pm)-RBS-tetR-DT (2) | 8/21 Plusgrow medium (+Amp)
|
| 8/21 Ptet(pm) control (1) | 8/21 Plusgrow medium (+Amp)
|
| 8/21 Ptet(pm) control (2) | 8/21 Plusgrow medium (+Amp)
|
| 8/21 Plux-RBS-GFP-DT (1) | 8/21 Plusgrow medium (+Amp)
|
| 8/21 pSB1C3(BBa_J04450) (1) | 8/21 Plusgrow medium (+Amp)
|
| 8/21 pSB1C3(BBa_J04450) (2) | 8/21 Plusgrow medium (+Amp)
|
incubate 37°C 10hour
Colony PCR
Kojima
| Sample | base pair
|
| 8/21 RBS-lysis1(1) | 400
|
| 8/21 RBS-lysis1(2) | 400
|
| 8/21 RBS control(1) | --
|
| 8/21 RBS control(2) | --
|
| negative control | --
|
| PreDenature | Denature | Annealing | Extension | cycle
|
| 94°C | 94°C | 55°C | 68°C | --
|
| 5min | 30s | 30s | 30s | 30cycles
|
| Sample | base pair
|
| 8/21 Pcon-GFP-DT-Pcon-RBS-luxR-DT (1) | 2143
|
| 8/21 Pcon-GFP-DT-Pcon-RBS-luxR-DT (2) | 2143
|
| 8/21 Pcon-RBS-luxR control(1) | --
|
| 8/21 Pcon-RBS-luxR control(2) | --
|
| negative control | --
|
| PreDenature | Denature | Annealing | Extension | cycle
|
| 94°C | 94°C | 55°C | 68°C | --
|
| 5min | 30s | 30s | 2min | 30cycles
|
| Sample | base pair
|
| 8/21 Ptet-RBS-tetR-DT (1) | 1216
|
| 8/21 Ptet-RBS-tetR-DT (2) | 1216
|
| 8/21 Ptet control (1) | --
|
| 8/21 Ptet control (2) | --
|
| 8/21 Plux-RBS-GFP-DT (1) | 1227
|
| 8/21 pSB1C3(BBa_J04450) (1) | 1353
|
| 8/21 pSB1C3(BBa_J04450) (2) | 1353
|
| negative control | --
|
| PreDenature | Denature | Annealing | Extension | cycle
|
| 94°C | 94°C | 55°C | 68°C | --
|
| 5min | 30s | 30s | 1min | 30cycles
|
Electrophoresis
No name
| Lane | Sample | Enzyme1 | Enzyme2
|
| 1 | pT181antisense | EcoRI | SpeI
|
| 2 | pT181antisense | EcoRI | --
|
| 3 | pT181antisense | -- | SpeI
|
| 4 | pT181antisense | -- | --
|
| 5 | pT181antisense | XbaI | PstI
|
| 6 | pT181antisense | XbaI | --
|
| 7 | pT181antisense | -- | PstI
|
| 8 | pT181antisense | -- | --
|
| 9 | 100bp ladder | -- | --
|
| 10 | Fusion1 attenuator | EcoRI | SpeI
|
| 11 | Fusion1 attenuator | EcoRI | --
|
| 12 | Fusion1 attenuator | -- | SpeI
|
| 13 | Fusion1 attenuator | -- | --
|
| 14 | Fusion1 attenuator | XbaI | PstI
|
| 15 | Fusion1 attenuator | XbaI | --
|
| 16 | fusion1 attenuator | -- | PstI
|
| 17 | fusion1 attenuator | -- | --
|
File:Igku xxxxxx.xxx
| Lane | Sample | Enzyme1 | Enzyme2
|
| 1 | Fusion3 3m2 attenuator | EcoRI | SpeI
|
| 2 | Fusion3 3m2 attenuator | EcoRI | --
|
| 3 | Fusion3 3m2 attenuator | -- | SpeI
|
| 4 | Fusion3 3m2 attenuator | -- | --
|
| 5 | Fusion3 3m2 attenuator | XbaI | PstI
|
| 6 | Fusion3 3m2 attenuator | XbaI | --
|
| 7 | Fusion3 3m2 attenuator | -- | PstI
|
| 8 | Fusion3 3m2 attenuator | -- | --
|
| 9 | 100bp ladder | -- | --
|
| 10 | aptamer 12-1M | EcoRI | SpeI
|
| 11 | aptamer 12-1M | EcoRI | --
|
| 12 | aptamer 12-1M | -- | SpeI
|
| 13 | aptamer 12-1M | -- | --
|
| 14 | aptamer 12-P | EcoRI | SpeI
|
| 15 | aptamer 12-P | EcoRI | --
|
| 16 | aptamer 12-1P | -- | SpeI
|
| 17 | aptamer 12-1P | -- | --
|
File:Igku xxxxxx.xxx
Restriction Enzyme Digestion
Miniprep
Electrophoresis
"
