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Team:Paris Saclay/Notebook/July/4
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===''Summary: ''=== | ===''Summary: ''=== | ||
| - | For regulator system: | + | <div>For regulator system: |
*estimated the size of segments for bands of electrophoresis by using Clone Manager. | *estimated the size of segments for bands of electrophoresis by using Clone Manager. | ||
*Made interpretation for electrophoresis. The picture shows that the experimental result was coherent with our estimation. We constructed our first BrioBrick, BBa_K1155000 (fnr+plasmid PSB1C3). | *Made interpretation for electrophoresis. The picture shows that the experimental result was coherent with our estimation. We constructed our first BrioBrick, BBa_K1155000 (fnr+plasmid PSB1C3). | ||
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*Seeded the 4 additional broths (2 for amilCP, 2 for FNR) for plasmid extraction(mini prep). | *Seeded the 4 additional broths (2 for amilCP, 2 for FNR) for plasmid extraction(mini prep). | ||
For PCBs sensor system: | For PCBs sensor system: | ||
| - | *Received the bacterial strain: pseudomonas KE707. | + | *Received the bacterial strain: pseudomonas KE707.</div> |
=='''Lab work'''== | =='''Lab work'''== | ||
Revision as of 08:37, 17 September 2013
Notebook : July 4
Summary:
For regulator system:
- estimated the size of segments for bands of electrophoresis by using Clone Manager.
- Made interpretation for electrophoresis. The picture shows that the experimental result was coherent with our estimation. We constructed our first BrioBrick, BBa_K1155000 (fnr+plasmid PSB1C3).
- stored 2 colonies who contain BioBrick BBa_K1155000
- the plasmid DNA extraction was performed for BBa_K1155000.
- The extract of plasmid DNA which contain BBa_K1155000 was digested by Not I, Mlu I, Hpa I, and were amplified by PCR.
- Seeded the 4 additional broths (2 for amilCP, 2 for FNR) for plasmid extraction(mini prep).
For PCBs sensor system:
- Received the bacterial strain: pseudomonas KE707.
Lab work
constructing
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