Template:Kyoto/Notebook/Aug 23

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===Gel Extraction===
===Gel Extraction===
 +
<div class="experiment">
 +
<span class="author">Nakamoto, Ashida, Kojima, Okazaki & Matsumoto</span>
 +
{| class="wikitable"
 +
!Lane||DNA||Enzyme
 +
|-
 +
|1||100bp ladder||--
 +
|-
 +
|2||rowspan=2|pT181 attenuator||rowspan=2|XbaI & PstI
 +
|-
 +
|3
 +
|-
 +
|5||rowspan=2|Fusion3m2 attenuator||rowspan=2|EcoRI & SpeI
 +
|-
 +
|6
 +
|-
 +
|8||rowspan=2|Fusion1 attenuator||rowspan=2|XbaI & PstI
 +
|-
 +
|9
 +
|-
 +
|11||rowspan=2|Fusion1 attenuator||rowspan=2|EcoRI & SpeI
 +
|-
 +
|12
 +
|}
 +
[[File:igku_xxbeforexx.xxx]]<br>
 +
[[File:igku_xxafterxx.xxx]]<br>
 +
{| class="wikitable"
 +
!Name||quantity||concentration[&micro;g/mL]||260/280||260/230
 +
|-
 +
|1|| || || ||
 +
|-
 +
|（ΦωΦ^）|| || || ||
 +
|}
 +
{| class="wikitable"
 +
!Lane||DNA||Enzyme
 +
|-
 +
|1||100bp ladder||--
 +
|-
 +
|2||rowspan=2|Fusion3m2 attenuator||rowspan=2|XbaI & PstI
 +
|-
 +
|3
 +
|-
 +
|5||rowspan=2|pT181 antisense||rowspan=2|EcoRI & SpeI
 +
|-
 +
|6
 +
|-
 +
|8||rowspan=2|pT181 antisense||rowspan=2|XbaI & PstI
 +
|-
 +
|9
 +
|-
 +
|11||rowspan=2|Fusion1 antisense||rowspan=2|XbaI & PstI
 +
|-
 +
|12
 +
|}
 +
[[File:igku_xxbeforexx.xxx]]<br>
 +
[[File:igku_xxafterxx.xxx]]<br>
 +
{| class="wikitable"
 +
!Name||quantity||concentration[&micro;g/mL]||260/280||260/230
 +
|-
 +
|1|| || || ||
 +
|-
 +
|（ΦωΦ^）|| || || ||
 +
|}
 +
{| class="wikitable"
 +
!Lane||DNA||Enzyme
 +
|-
 +
|1||100bp ladder||--
 +
|-
 +
|2||rowspan=2|Fusion6 attenuator||rowspan=2|XbaI & PstI
 +
|-
 +
|3
 +
|-
 +
|5||rowspan=2|tetR aptamer 12_1M||rowspan=2|EcoRI & SpeI
 +
|-
 +
|6
 +
|}
 +
{| class="wikitable"
 +
!Lane||DNA||Enzyme
 +
|-
 +
|1||100bp ladder||--
 +
|-
 +
|2||rowspan=2|tetR aptamer 12_P||rowspan=2|EcoRI & SpeI
 +
|-
 +
|3
 +
|-
 +
|5||rowspan=2|tetR aptamer 12_1R||rowspan=2|EcoRI & SpeI
 +
|-
 +
|6
 +
|}
 +
[[File:igku_xxbeforexx.xxx]]<br>
 +
[[File:igku_xxafterxx.xxx]]<br>
 +
{| class="wikitable"
 +
!Name||quantity||concentration[&micro;g/mL]||260/280||260/230
 +
|-
 +
|1|| || || ||
 +
|-
 +
|（ΦωΦ^）|| || || ||
 +
|}
 +
<span class="author">Ashida</span>
 +
{| class="wikitable"
 +
!Lane||DNA||Enzyme
 +
|-
 +
|1||100bp ladder||--
 +
|-
 +
|2||rowspan=2|Fusion6 antisense||rowspan=2|EcoRI & SpeI
 +
|-
 +
|3
 +
|-
 +
|5||rowspan=2|Fusion6 attenuator||rowspan=2|XbaI & PstI
 +
|-
 +
|6
 +
|-
 +
|8||rowspan=2|Fusion1 antisense||rowspan=2|EcoRI & SpeI
 +
|-
 +
|9
 +
|-
 +
|11||rowspan=2|pT181 antisense||rowspan=2|EcoRI & SpeI
 +
|-
 +
|12
 +
|}
 +
[[File:igku_xxbeforexx.xxx]]<br>
 +
[[File:igku_xxafterxx.xxx]]<br>
 +
{| class="wikitable"
 +
!Name||quantity||concentration[&micro;g/mL]||260/280||260/230
 +
|-
 +
|1|| || || ||
 +
|-
 +
|（ΦωΦ^）|| || || ||
 +
|}
 +
{| class="wikitable"
 +
!Lane||DNA||Enzyme
 +
|-
 +
|1||100bp ladder||--
 +
|-
 +
|3||rowspan=2|pT181 antisense||rowspan=2|XbaI & PstI
 +
|-
 +
|4
 +
|}
 +
[[File:igku_xxbeforexx.xxx]]<br>
 +
[[File:igku_xxafterxx.xxx]]<br>
 +
{| class="wikitable"
 +
!Name||quantity||concentration[&micro;g/mL]||260/280||260/230
 +
|-
 +
|1|| || || ||
 +
|-
 +
|（ΦωΦ^）|| || || ||
 +
|}
 +
{| class="wikitable"
 +
!Lane||DNA||Enzyme
 +
|-
 +
|1||100bp ladder||--
 +
|-
 +
|3||rowspan=2|Plac||rowspan=2|SpeI & PstI
 +
|-
 +
|4
 +
|}
 +
{| class="wikitable"
 +
!Lane||DNA||Enzyme
 +
|-
 +
|1||100bp ladder||--
 +
|-
 +
|3||rowspan=2|J23100||rowspan=2|SpeI & PstI
 +
|-
 +
|5||rowspan=2|Spinach||rowspan=2|EcoRI & SpeI
 +
|-
 +
|6
 +
|}
 +
[[File:igku_xxbeforexx.xxx]]<br>
 +
[[File:igku_xxafterxx.xxx]]<br>
 +
{| class="wikitable"
 +
!Name||quantity||concentration[&micro;g/mL]||260/280||260/230
 +
|-
 +
|1|| || || ||
 +
|-
 +
|（ΦωΦ^）|| || || ||
 +
|}
 +
 +
</div>

Revision as of 15:19, 17 September 2013

Contents

Aug 23

Electrophoresis

No name

LaneSampleEnzyme1Enzyme2
1pT181 antisenseEcoRISpeI
2pT181 antisenseEcoRI--
3pT181 antisense--SpeI
4pT181 antisense----
5pT181 antisenseXbaIPstI
6pT181 antisenseXbaI--
7pT181 antisense--PstI
8pT181 antisense----
9100bp ladder----
10Fusion1 attenuatorEcoRISpeI
11Fusion1 attenuatorEcoRI--
12Fusion1 attenuator--SpeI
13Fusion1 attenuator----
14Fusion1 attenuatorXbaIPstI
15Fusion1 attenuatorXbaI--
16Fusion1 attenuator--PstI
17Fusion1 attenuator----

File:Igku xxxxxx.xxx

LaneSampleEnzyme1Enzyme2
1Fusion3m2 attenuatorEcoRISpeI
2Fusion3m2 attenuatorEcoRI--
3Fusion3m2 attenuator--SpeI
4Fusion3m2 attenuator----
5Fusion3m2 attenuatorXbaIPstI
6Fusion3m2 attenuatorXbaI--
7Fusion3m2 attenuator--PstI
8Fusion3m2 attenuator----
9100bp ladder----
10tetR aptamer 12_1MEcoRISpeI
11tetR aptamer 12_1MEcoRI--
12tetR aptamer 12_1M--SpeI
13tetR aptamer 12_1M----
14tetR aptamer 12_PEcoRISpeI
15tetR aptamer 12_PEcoRI--
16tetR aptamer 12_P--SpeI
17tetR aptamer 12_P----

File:Igku xxxxxx.xxx
Kojima and Honda

LaneSampleEnzyme1Enzyme2
1PlacSpeIPstI
2PlacSpeI--
3Plac--PstI
4Plac----
5SpinachEcoRISpeI
6SpinachEcoRI--
7Spinach--SpeI
8Spinach----
9100bp ladder----
10J23100SpeIPstI
11J23100SpeI--
12J23100--PstI
13J23100----
14tetR aptamer 12_1REcoRISpeI
15tetR aptamer 12_1REcoRI--
16tetR aptamer 12_1R--SpeI
17tetR aptamer 12_1R----

File:Igku xxxxxx.xxx

Restriction Enzyme Digestion

Nakamoto and Kojima

Fusion6 antisense (1)EcoRISpeIbuffer BBSAMilliQtotal
30.50.530.322.730
0.60.1010.18.210
0.600.110.18.210
0.60010.18.310
Fusion6 antisense (1)XbaIPstIbuffer DBSAMilliQtotal
30.50.530.322.730
0.60.1010.18.210
0.600.110.18.210
0.60010.18.310
Fusion1 antisense (1)EcoRISpeIbuffer BBSAMilliQtotal
3.30.50.530.322.430
0.70.1010.18.110
0.700.110.18.110
0.70010.18.210
pT181 antisense (1)EcoRISpeIbuffer BBSAMilliQtotal
5.60.50.530.320.130
1.10.1010.17.710
1.100.110.17.710
1.10010.17.810
pT181 antisense (1)XbaIPstIbuffer DBSAMilliQtotal
5.60.50.530.320.130
1.10.1010.17.710
1.100.110.17.710
1.10010.17.810
  • incubate at 37°C from 17:29.

Electrophoresis

No name

LaneSampleEnzyme1Enzyme2
1100bp----
2pT181 attenuatorEcoRISpeI
3pT181 attenuatorXbaIPstI
4Fusion3m2 attenuatorEcoRISpeI
5Fusion1 attenuatorXbaIPstI
6Fusion1 attenuatorEcoRISpeI
7Fusion3m2 attenuatorXbaIPstI
8pT181 antisenseEcoRISpeI
9pT181 antisenseXbaIPstI
10Fusion1 antisenseEcoRISpeI
11Fusion1 antisenseXbaIPstI
12Fusion6 antisenseEcoRISpeI
13Fusion6 antisenseXbaIPstI
14tetR aptamer 12_1MEcoRISpeI
15tetR aptamer 12_PEcoRISpeI
16tetR aptamer 12_1REcoRISpeI
17100bp----

File:Igku xxxxxx.xxx

LaneSampleEnzyme1Enzyme2
1100bp----
2SpinachEcoRISpeI
3J23100SpeIPstI
4PlacSpeIPstI
5100bp----

File:Igku xxxxxx.xxx

LaneSampleEnzyme1Enzyme2
1100bp----
2Fusion6 antisenseEcoRISpeI
3Fusion6 antisenseEcoRI--
4Fusion6 antisense--SpeI
5Fusion6 antisense----
6pT181 antisenseXbaIPstI
8100bp----
9100bp----
10Fusion6 antisenseXbaIPstI
11Fusion6 antisenseXbaI--
12Fusion6 antisense--PstI
13Fusion6 antisense----
14pT181 antisenseXbaI--
16100bp----

File:Igku xxxxxx.xxx

LaneSampleEnzyme1Enzyme2
1100bp----
2Fusion1 antisenseEcoRISpeI
3Fusion1 antisenseEcoRI--
4Fusion1 antisense--SpeI
5Fusion1 antisense----
6pT181 antisense--PstI
8100bp----
9100bp----
10pT181 antisenseEcoRISpeI
11pT181 antisenseEcoRI--
12pT181 antisense--SpeI
13pT181 antisense----
14pT181 antisense----
16100bp----

File:Igku xxxxxx.xxx

Gel Extraction

Nakamoto, Ashida, Kojima, Okazaki & Matsumoto

LaneDNAEnzyme
1100bp ladder--
2pT181 attenuatorXbaI & PstI
3
5Fusion3m2 attenuatorEcoRI & SpeI
6
8Fusion1 attenuatorXbaI & PstI
9
11Fusion1 attenuatorEcoRI & SpeI
12

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
1
（ΦωΦ^）
LaneDNAEnzyme
1100bp ladder--
2Fusion3m2 attenuatorXbaI & PstI
3
5pT181 antisenseEcoRI & SpeI
6
8pT181 antisenseXbaI & PstI
9
11Fusion1 antisenseXbaI & PstI
12

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
1
（ΦωΦ^）
LaneDNAEnzyme
1100bp ladder--
2Fusion6 attenuatorXbaI & PstI
3
5tetR aptamer 12_1MEcoRI & SpeI
6
LaneDNAEnzyme
1100bp ladder--
2tetR aptamer 12_PEcoRI & SpeI
3
5tetR aptamer 12_1REcoRI & SpeI
6

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
1
（ΦωΦ^）

Ashida

LaneDNAEnzyme
1100bp ladder--
2Fusion6 antisenseEcoRI & SpeI
3
5Fusion6 attenuatorXbaI & PstI
6
8Fusion1 antisenseEcoRI & SpeI
9
11pT181 antisenseEcoRI & SpeI
12

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
1
（ΦωΦ^）
LaneDNAEnzyme
1100bp ladder--
3pT181 antisenseXbaI & PstI
4

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
1
（ΦωΦ^）
LaneDNAEnzyme
1100bp ladder--
3PlacSpeI & PstI
4
LaneDNAEnzyme
1100bp ladder--
3J23100SpeI & PstI
5SpinachEcoRI & SpeI
6

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
1
（ΦωΦ^）
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