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ds ='på dig';

}

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else if(id == 'd2013721')

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else if(id == 'd2013721')

{

ds = '<div id="dairy-text"><h1>Sunday 2013-07-21</h1>Name of participants:Hampus.E, Theodor L, Karl H, Ken B, Kristoffer L, Viktor @, Marcus H. Nils, Christoffer, Anders Edlund (AE) '+

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'<h2>Ongoing constructs:</h3>104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL 121.pSB1C3-B0034-STS 121.pSB1C3-B0034-His-STS '+

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'<h2>Ongoing constructs:</h2>104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL 121.pSB1C3-B0034-STS 121.pSB1C3-B0034-His-STS '+

'99. pSB1C3-CrtO 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His 129. pSB1C3-Idi (mut) 21. CrtE~I /w RBS, Zinc finger (PCR prod) 128. pSIM5-tet(Nissle) 130. pSB1C3-CrtE~I /w RBS, Zinc Finger 131. pSB4A15-J23101-CrtE~I /w RBS, Zinc Finger 132. pSB4A15-J23106-CrtE~I /w RBS, Zinc Finger 133. pSB4A15 -J23110-CrtE~I /w RBS, Zinc Finger 7.pSB3k3-red 22. B0032-BFP 42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 84. pSB4S15-CP6 85. pSB4S15-CP25 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 90. BBa_J61002-J23101 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 100. pJP059 111. pSBLb4C15 112. pSBLbEc4C15 136.1 pSB1C3-usp45 '+

'L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 Lb. delbrueckii bulgaricus: 18. Lb. delbrueckii bulgaricus Lc. thermophilus: 19. Lc.thermophilus '+

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'<h1>Todays work:</~~h1><br~~>Plasmid Prep on: 117.2 pSB3T5-CP1-B0034-His-TAL 119.2 pSB1C3-B0034-4CL 120.1 pSB1C3-B0034-HIS-4CL 104.2 and 104.5 pSB1C3-B0034-4CL-B0034-STS 106.5 and 106.6 pSB3T5-J23110-B0034-TAL 122.2 pSB1C3-B0034-His-STS; Re-streak: 131. pSB4A15-BBa-J61002-J23106-CrtE~I /w RBS, Zinc finger 132. pSB4A15-BBa-J61002-J23110-CrtE~I /w RBS, Zinc finger 133. pSB4A15-BBa-J61002-J23101-CrtE~I /w RBS, Zinc finger 134.1 pSB1C3-CBP 134.2 pSB1C3-CBP 134.3 pSB1C3-CBP 134.4 pSB1C3-CBP 136.1 pSB1C3-usp45 136.2 pSB1C3-usp45 136.3 pSB1C3-usp45 136.4 pSB1C3-usp45 Gel: 21. CrtE~I/w RBS,ZF Overnight & screening: 99. pSB1C3-CrtO 128.pSIM5-tet (E.Coli Nissle) Endast O/N 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA '+

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'<h2>Todays work:</h2>Plasmid Prep on: 117.2 pSB3T5-CP1-B0034-His-TAL 119.2 pSB1C3-B0034-4CL 120.1 pSB1C3-B0034-HIS-4CL 104.2 and 104.5 pSB1C3-B0034-4CL-B0034-STS 106.5 and 106.6 pSB3T5-J23110-B0034-TAL 122.2 pSB1C3-B0034-His-STS; Re-streak: 131. pSB4A15-BBa-J61002-J23106-CrtE~I /w RBS, Zinc finger 132. pSB4A15-BBa-J61002-J23110-CrtE~I /w RBS, Zinc finger 133. pSB4A15-BBa-J61002-J23101-CrtE~I /w RBS, Zinc finger 134.1 pSB1C3-CBP 134.2 pSB1C3-CBP 134.3 pSB1C3-CBP 134.4 pSB1C3-CBP 136.1 pSB1C3-usp45 136.2 pSB1C3-usp45 136.3 pSB1C3-usp45 136.4 pSB1C3-usp45 Gel: 21. CrtE~I/w RBS,ZF Overnight & screening: 99. pSB1C3-CrtO 128.pSIM5-tet (E.Coli Nissle) Endast O/N 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA '+

'Overnight: 84.1 pSB4S15-CP6 84.2 pSB4S15-CP6 86.1 pSB4S15-CP6-B0032-BFP 86.2 pSB4S15-CP6-B0032-BFP 87(1.1) pSB4S15-CP25-B0032-BFP 87(1.2) pSB4S15-CP25-B0032-BFP 88.1 pSB4S15-CP1-B0032-BFP 88.2 pSB4S15-CP1-B0032-BFP 89.1 pSB4S15-CP44-B0032-BFP 89.2 pSB4S15-CP44-B0032-BFP 97.1 pSB4S15-CP8-B0032-BFP 97.2 pSB4S15-CP8-B0032-BFP 98.1 pSB4S15-CP11-B0032-BFP 98.2 pSB4S15-CP11-B0032-BFP '+

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'<h1>Results:</h1> Re-streak: 131. pSB4A15-BBa-J61002-J23106-CrtE~I /w RBS, Zinc finger - Unable, no clones 132. pSB4A15-BBa-J61002-J23110-CrtE~I /w RBS, Zinc finger - 3 clones restreaked 133. pSB4A15-BBa-J61002-J23101-CrtE~I /w RBS, Zinc finger - 3 clones restreaked Gel 21. CrtE~I/w RBS,ZF - fail Overnight & screening: 99. pSB1C3-CrtO 108. pSB4A15-J23106-B0034-IspA - Fail of all clones 109. pSB4A15-K206000-B0034-IspA - Fail of all clones 110. pSB4A15-K206001-B0034-IspA - Fail of all clones </div>';

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'<h2>Results:</h2> Re-streak: 131. pSB4A15-BBa-J61002-J23106-CrtE~I /w RBS, Zinc finger - Unable, no clones 132. pSB4A15-BBa-J61002-J23110-CrtE~I /w RBS, Zinc finger - 3 clones restreaked 133. pSB4A15-BBa-J61002-J23101-CrtE~I /w RBS, Zinc finger - 3 clones restreaked Gel 21. CrtE~I/w RBS,ZF - fail Overnight & screening: 99. pSB1C3-CrtO 108. pSB4A15-J23106-B0034-IspA - Fail of all clones 109. pSB4A15-K206000-B0034-IspA - Fail of all clones 110. pSB4A15-K206001-B0034-IspA - Fail of all clones </div>';

}

else if(id == 'd2013720')

{

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ds = '<div id="dairy-text"><h1>Saturday 2013-07-20</h1>Name of participants: Hampus.E, Kristoffer.L, Malin .B, Magnus, Nils, Pontus, Anders Edlund (AE), Stephanie Herman (SH) <h2>Ongoing constructs:</~~h3><br~~>99. pSB1C3-CrtO 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His 129. pSB1C3-Idi (mut) 21. CrtE~I /w RBS, Zinc finger (PCR prod) 128. pSIM5-tet(Nissle) 130. pSB1C3-CrtE~I /w RBS, Zinc Finger 131. pSB4A15-J23101-CrtE~I /w RBS, Zinc Finger 132. pSB4A15-J23106-CrtE~I /w RBS, Zinc Finger 133. pSB4A15 -J23110-CrtE~I /w RBS, Zinc Finger 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL 121.pSB1C3-B0034-STS 121.pSB1C3-B0034-His-STS 7.pSB3k3-red 22. B0032-BFP 42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 84. pSB4S15-CP6 85. pSB4S15-CP25 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 90. BBa_J61002-J23101 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 100. pJP059 111. pSBLb4C15 112. pSBLbEc4C15 136.1 pSB1C3-usp45 L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 Lb. delbrueckii bulgaricus: 18. Lb. delbrueckii bulgaricus Lc. thermophilus: 19. Lc.thermophilus <h2>Todays work</~~h3> <br~~>Re-streak: 84. pSB4S15-CP6 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 99. pSB1C3-CrtO Overnigth: 136.1 pSB1C3-usp45 136.2 pSB1C3-usp45 111.6 pSBLb4C15-red 111.7 pSBLb4C15-red Transformation: 131. pSB4A15-BBa-J61002-J23106-CrtE~I /w RBS, Zinc finger 132. pSB4A15-BBa-J61002-J23110-CrtE~I /w RBS, Zinc finger 133. pSB4A15-BBa-J61002-J23101-CrtE~I /w RBS, Zinc finger 134. pSB1C3-CBP 135. pSB1C3-CSP 136. pSB1C3-USP45 Plasmidprep: 109. pSB4A15-K206000-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His 1.1? 90.3 j23101 1.2? 2.1?90.4 j23101 2.2? Gel: 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA Screening pcr of constructs: 119.pSB1C3-B0034-4CL 120 pSB1C3-B0034-HIS-4CL 106 pSB3T5-J23110-B0034-TAL 117 pSB3T5-CP1-B0034-His-TAL 107 pSB3T5-CP1-B0034-TAL PCR: 21. pSB4C5-pBAD/AraC-CrtE~I/w RBS, ZF (to remove promotor) 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA <h2>Results</~~h3><br~~>Overnight & screening: 99. pSB1C3-CrtO 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA Screening of: 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA showed negative results on all clones but 109.2. A new screening PCR of previously un-screened clones was prepared to run overnight.Gel Gel succes on: 106.5 pSB3T5-J23110-B0034-TAL 106.6 pSB3T5-J23110-B0034-TAL 117.1 pSB3T5-CP1-B0034-His-TAL 117.2 pSB3T5-CP1-B0034-His-TAL 119.2 pSB1C3-B0034-4CL No band on: 107 pSB3T5-CP1-B0034-TAL 116 pSB3T5-J23110-B0034-His-TAL 120 pSB1C3-B0034-HIS-4CL </div>';

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ds = '<div id="dairy-text"><h1>Saturday 2013-07-20</h1>Name of participants: Hampus.E, Kristoffer.L, Malin .B, Magnus, Nils, Pontus, Anders Edlund (AE), Stephanie Herman (SH) <h2>Ongoing constructs:</h2>99. pSB1C3-CrtO 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His 129. pSB1C3-Idi (mut) 21. CrtE~I /w RBS, Zinc finger (PCR prod) 128. pSIM5-tet(Nissle) 130. pSB1C3-CrtE~I /w RBS, Zinc Finger 131. pSB4A15-J23101-CrtE~I /w RBS, Zinc Finger 132. pSB4A15-J23106-CrtE~I /w RBS, Zinc Finger 133. pSB4A15 -J23110-CrtE~I /w RBS, Zinc Finger 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL 121.pSB1C3-B0034-STS 121.pSB1C3-B0034-His-STS 7.pSB3k3-red 22. B0032-BFP 42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 84. pSB4S15-CP6 85. pSB4S15-CP25 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 90. BBa_J61002-J23101 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 100. pJP059 111. pSBLb4C15 112. pSBLbEc4C15 136.1 pSB1C3-usp45 L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 Lb. delbrueckii bulgaricus: 18. Lb. delbrueckii bulgaricus Lc. thermophilus: 19. Lc.thermophilus <h2>Todays work</h2>Re-streak: 84. pSB4S15-CP6 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 99. pSB1C3-CrtO Overnigth: 136.1 pSB1C3-usp45 136.2 pSB1C3-usp45 111.6 pSBLb4C15-red 111.7 pSBLb4C15-red Transformation: 131. pSB4A15-BBa-J61002-J23106-CrtE~I /w RBS, Zinc finger 132. pSB4A15-BBa-J61002-J23110-CrtE~I /w RBS, Zinc finger 133. pSB4A15-BBa-J61002-J23101-CrtE~I /w RBS, Zinc finger 134. pSB1C3-CBP 135. pSB1C3-CSP 136. pSB1C3-USP45 Plasmidprep: 109. pSB4A15-K206000-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His 1.1? 90.3 j23101 1.2? 2.1?90.4 j23101 2.2? Gel: 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA Screening pcr of constructs: 119.pSB1C3-B0034-4CL 120 pSB1C3-B0034-HIS-4CL 106 pSB3T5-J23110-B0034-TAL 117 pSB3T5-CP1-B0034-His-TAL 107 pSB3T5-CP1-B0034-TAL PCR: 21. pSB4C5-pBAD/AraC-CrtE~I/w RBS, ZF (to remove promotor) 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA <h2>Results</h2>Overnight & screening: 99. pSB1C3-CrtO 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA Screening of: 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA showed negative results on all clones but 109.2. A new screening PCR of previously un-screened clones was prepared to run overnight.Gel Gel succes on: 106.5 pSB3T5-J23110-B0034-TAL 106.6 pSB3T5-J23110-B0034-TAL 117.1 pSB3T5-CP1-B0034-His-TAL 117.2 pSB3T5-CP1-B0034-His-TAL 119.2 pSB1C3-B0034-4CL No band on: 107 pSB3T5-CP1-B0034-TAL 116 pSB3T5-J23110-B0034-His-TAL 120 pSB1C3-B0034-HIS-4CL </div>';

}

else if(id == 'd2013719')

{

-

ds = '<div id="dairy-text"><h1>Friday 2013-07-19</h1> Name of participants: Ken B-A, Kristoffer L, Malin, Magnus, Sabri, Nils, Pontus, Christoffer Ahlström (CA), Stephanie Herman (SH) <h2>Ongoing constructs:</~~h3><br~~>E.coli (strain D5α): 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His 129. pSB1C3-Idi (mut) 21. CrtE~I /w RBS, Zinc finger (PCR prod) 128. pSIM5-tet(Nissle) 130. pSB1C3-CrtE~I /w RBS, Zinc Finger 131. pSB4A15-J23101-CrtE~I /w RBS, Zinc Finger 132. pSB4A15-J23106-CrtE~I /w RBS, Zinc Finger 133. pSB4A15 -J23110-CrtE~I /w RBS, Zinc Finger 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 7.pSB3k3-red 42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 84. pSB4S15-CP6 85. pSB4S15-CP25 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 90. BBa_J61002-J23101 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 100. pJP059 111. pSBLb4C15 112. pSBLbEc4C15 136.1 pSB1C3-usp45 L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 <h2>Todays work</~~h3><br~~>Screening pcr of constructs: 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 117. pSB3T5-CP1-B0034-His-Tal 119. pSB1C3-B0034-4CL (from 15) 120. pSB1C3-B0034-His-4CL (from 15) Overnight & screening: 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His Plasmid preparation: 122.2. pSB1C3-B0034-His-STS (from 15) 90.3. BBa_J61002 (amp)-J23101 90.4. BBa_J61002 (amp)-J23101 104.2. pSB1C3-B0034-4CL-B0034-STS 104.5. pSB1C3-B0034-4CL-B0034-STS 111.2. pSBLb4C15-E.coli ori replaced with ori from lactobacillus (pJP059) in pEL4C15 111.3. pSBLb4C15-E.coli ori replaced with ori from lactobacillus (pJP059) in pEL4C15 111.4. pSBLb4C15-E.coli ori replaced with ori from lactobacillus (pJP059) in pEL4C15 121.1. pSB1C3-B0034-STS (from 15) 121.2. pSB1C3-B0034-STS (from 15) Cloning: 21. CrtE~I /w RBS, Zinc finger → 1. pSB1C3-red Assembly: 21. CrtE~I /w RBS, Zinc finger, 91. BBa-J61002-J23106 → 11. pSB4A15-red 21. CrtE~I /w RBS, Zinc finger, 92. BBa-J61002-J23110 → 11. pSB4A15-red 21. CrtE~I /w RBS, Zinc finger, 103. BBa-J61002-J23101 → 11. pSB4A15-red <Gel electrophoresis: 129. pSB1C3-Idi (mut) Restreak 128. pSIM5-tet(Nissle) <h2>Results:</~~h3><br~~>Screening: 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His showed negative results on all clones but 109.2, which proceeded to overnight. A new screening PCR was prepared to run overnight. showed positive results in all cases but 125.4 and 127.3. All other clones proceeded to overnight. The overnight showed no contamination of negative controls, and growth in all clones but 127.1 and 127.2. Transformation: Only visual red colonies on CBP & CSP and possible 2 white colonies on usp45. → Re-do the transformation with the saved ligation solution from (18/07-13) → Re-streak the possible white colonies. </div>';

+

ds = '<div id="dairy-text"><h1>Friday 2013-07-19</h1> Name of participants: Ken B-A, Kristoffer L, Malin, Magnus, Sabri, Nils, Pontus, Christoffer Ahlström (CA), Stephanie Herman (SH) <h2>Ongoing constructs:</h2>E.coli (strain D5α): 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His 129. pSB1C3-Idi (mut) 21. CrtE~I /w RBS, Zinc finger (PCR prod) 128. pSIM5-tet(Nissle) 130. pSB1C3-CrtE~I /w RBS, Zinc Finger 131. pSB4A15-J23101-CrtE~I /w RBS, Zinc Finger 132. pSB4A15-J23106-CrtE~I /w RBS, Zinc Finger 133. pSB4A15 -J23110-CrtE~I /w RBS, Zinc Finger 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 7.pSB3k3-red 42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 84. pSB4S15-CP6 85. pSB4S15-CP25 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 90. BBa_J61002-J23101 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 100. pJP059 111. pSBLb4C15 112. pSBLbEc4C15 136.1 pSB1C3-usp45 L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 <h2>Todays work</h2>Screening pcr of constructs: 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 117. pSB3T5-CP1-B0034-His-Tal 119. pSB1C3-B0034-4CL (from 15) 120. pSB1C3-B0034-His-4CL (from 15) Overnight & screening: 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His Plasmid preparation: 122.2. pSB1C3-B0034-His-STS (from 15) 90.3. BBa_J61002 (amp)-J23101 90.4. BBa_J61002 (amp)-J23101 104.2. pSB1C3-B0034-4CL-B0034-STS 104.5. pSB1C3-B0034-4CL-B0034-STS 111.2. pSBLb4C15-E.coli ori replaced with ori from lactobacillus (pJP059) in pEL4C15 111.3. pSBLb4C15-E.coli ori replaced with ori from lactobacillus (pJP059) in pEL4C15 111.4. pSBLb4C15-E.coli ori replaced with ori from lactobacillus (pJP059) in pEL4C15 121.1. pSB1C3-B0034-STS (from 15) 121.2. pSB1C3-B0034-STS (from 15) Cloning: 21. CrtE~I /w RBS, Zinc finger → 1. pSB1C3-red Assembly: 21. CrtE~I /w RBS, Zinc finger, 91. BBa-J61002-J23106 → 11. pSB4A15-red 21. CrtE~I /w RBS, Zinc finger, 92. BBa-J61002-J23110 → 11. pSB4A15-red 21. CrtE~I /w RBS, Zinc finger, 103. BBa-J61002-J23101 → 11. pSB4A15-red <Gel electrophoresis: 129. pSB1C3-Idi (mut) Restreak 128. pSIM5-tet(Nissle) <h2>Results:</h2>Screening: 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His showed negative results on all clones but 109.2, which proceeded to overnight. A new screening PCR was prepared to run overnight. showed positive results in all cases but 125.4 and 127.3. All other clones proceeded to overnight. The overnight showed no contamination of negative controls, and growth in all clones but 127.1 and 127.2. Transformation: Only visual red colonies on CBP & CSP and possible 2 white colonies on usp45. → Re-do the transformation with the saved ligation solution from (18/07-13) → Re-streak the possible white colonies. </div>';

}

else if(id == 'd2013718')

{

-

ds = '<div id="dairy-text"> <h1>Thursday 2013-07-18</h1> Name of participants: Karl H, Ken B-A, Kristoffer L, Christoffer, Alexander, Magnus, Pontus,Sabri,StephanieHerman (SH), Alona Nyberg (AN), Christoffer Ahlström (CA), Viktor Blomkvist (VB) <h2>Ongoing constructs:</~~h3> <br~~>E.coli (strain D5α): 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 7.pSB3k3-red 22. B0032-BFP 42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 84. pSB4S15-CP6 85. pSB4S15-CP25 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 90. BBa_J61002-J23101 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 100. pJP059 111. pSBLb4C15 112. pSBLbEc4C15 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His 129. pSB1C3-Idi (mut) 113. E.coli Nissle-pSIM5-tet L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 Lb. delbrueckii bulgaricus: 18. Lb. delbrueckii bulgaricus Lc. thermophilus: 19. Lc.thermophilus <h2>Todays work</~~h3> <br~~>Assembly 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL 120. pSB1C3-B0034-His-4CL Ligation 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL 120. pSB1C3-B0034-His-4CL Gel-electrophoresis of screening PCR products 42. pSB4S15-red Digestion: 42. pSB4S15-red Transformation: 81. pSB4S15-CP41-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 84. pSB4S15-CP6 85. pSB4S15-CP25 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP Overnight: 90. BBa_J61002-J23101 Spreading of strains: 81. pSB4S15-CP41-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 84. pSB4S15-CP6 85. pSB4S15-CP25 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP Re-circulization: 129. pSB1C3-Idi (mut) Plasmid Prep (Glycerol stock): 75. pEL3A15 - CrtB (75.1, 75.2, 75.4) Transformation: 99. pSB1C3 - Crto 129. pSB1C3-Idi (mut) 113. E.coli Nissle-pSIM5-tet 111. pSBLb4C15 112. pSBLbEc4C15 Re-streak: 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His Ligation of strains from yesterdays digest: Collagen binding protein → cloned from strain 14.4 Cell surface protein → cloned from strain 3.1 usp45 → cloned from strain 13.1 *Ligated to 1.1 pSB1C3-red (From strain database 2013) Transformation:* Collagen binding protein Cell surface protein usp45 *Performed with D5(alpha) competent cells. Screening: 111. pSBLb4C15 Spreading of strains: Collagen binding protein [D5(alpha)] * Cell surface protein [D5(alpha)] * usp45 [D5(alpha)] * 111. pSBLb4C15 ** 112. pSBLbEc4C15 ** *With a 10x and 1x dilution. ** 2 plates of each with 1x dilution. <h2>Results</~~h3> <br~~>Re-streaks: 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His were successful on all clones but 108.7. Transformation of 129. pSB1C3-Idi (mut) did not grow. Electrophoresis on digests: *The gel showed that the components (signal peptides and destination plasmid) had been cut and therefore have the digest succeeded. </div>';

+

ds = '<div id="dairy-text"> <h1>Thursday 2013-07-18</h1> Name of participants: Karl H, Ken B-A, Kristoffer L, Christoffer, Alexander, Magnus, Pontus,Sabri,StephanieHerman (SH), Alona Nyberg (AN), Christoffer Ahlström (CA), Viktor Blomkvist (VB) <h2>Ongoing constructs:</h2>E.coli (strain D5α): 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 7.pSB3k3-red 22. B0032-BFP 42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 84. pSB4S15-CP6 85. pSB4S15-CP25 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 90. BBa_J61002-J23101 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 100. pJP059 111. pSBLb4C15 112. pSBLbEc4C15 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His 129. pSB1C3-Idi (mut) 113. E.coli Nissle-pSIM5-tet L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 Lb. delbrueckii bulgaricus: 18. Lb. delbrueckii bulgaricus Lc. thermophilus: 19. Lc.thermophilus <h2>Todays work</h2>Assembly 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL 120. pSB1C3-B0034-His-4CL Ligation 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL 120. pSB1C3-B0034-His-4CL Gel-electrophoresis of screening PCR products 42. pSB4S15-red Digestion: 42. pSB4S15-red Transformation: 81. pSB4S15-CP41-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 84. pSB4S15-CP6 85. pSB4S15-CP25 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP Overnight: 90. BBa_J61002-J23101 Spreading of strains: 81. pSB4S15-CP41-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 84. pSB4S15-CP6 85. pSB4S15-CP25 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP Re-circulization: 129. pSB1C3-Idi (mut) Plasmid Prep (Glycerol stock): 75. pEL3A15 - CrtB (75.1, 75.2, 75.4) Transformation: 99. pSB1C3 - Crto 129. pSB1C3-Idi (mut) 113. E.coli Nissle-pSIM5-tet 111. pSBLb4C15 112. pSBLbEc4C15 Re-streak: 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His Ligation of strains from yesterdays digest: Collagen binding protein → cloned from strain 14.4 Cell surface protein → cloned from strain 3.1 usp45 → cloned from strain 13.1 *Ligated to 1.1 pSB1C3-red (From strain database 2013) Transformation:* Collagen binding protein Cell surface protein usp45 *Performed with D5(alpha) competent cells. Screening: 111. pSBLb4C15 Spreading of strains: Collagen binding protein [D5(alpha)] * Cell surface protein [D5(alpha)] * usp45 [D5(alpha)] * 111. pSBLb4C15 ** 112. pSBLbEc4C15 ** *With a 10x and 1x dilution. ** 2 plates of each with 1x dilution. <h2>Results</h2>Re-streaks: 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His were successful on all clones but 108.7. Transformation of 129. pSB1C3-Idi (mut) did not grow. Electrophoresis on digests: *The gel showed that the components (signal peptides and destination plasmid) had been cut and therefore have the digest succeeded. </div>';

}

else if(id == 'd2013717')

{

-

ds = '<div id="dairy-text"> <h1>Thursday 2013-07-18</h1> Name of participants: Karl H, Ken B-A, Kristoffer L, Christoffer, Sabri, Alexander, Nils, Thorsteinn, Magnus, Malin, Pontus, Niclas <h2>Ongoing constructs:</~~h3> <br~~>E.coli (strain D5α): 75. pEL3A15 - CrtB 99. pSB1C3 - CrtO 79. pEL3K16 - CrtE 102. pSB1C3 - CrtE 101. pSB1C3 - DNA program123456 96. Cs42s-Promoter-ZCD-PsbA2-UGTCs2-PsbA2-Beta-carotene hydroxylase-B1002 57.Psb1C3-B0034-Idi 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 7.pSB3k3-red 22. B0032-BFP 42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 100. pJP059 111. pSBLb4C15 112. pSBLbEc4C15 L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 Lb. delbrueckii bulgaricus: 18. Lb. delbrueckii bulgaricus Lc. thermophilus: 19. Lc.thermophilus <h2>Todays work</~~h3> <br~~>Assembly 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL 120. pSB1C3-B0034-His-4CL Ligation 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL 120. pSB1C3-B0034-His-4CL Digest & Ligation: ZCD + 1. pSB1C3-Red -> 123. pSB1C3-ZCD ZCD-His + 1. pSB1C3-Red -> 125. pSB1C3-ZCD-His UGTCs2 + 1. pSB1C3-Red -> 126. pSB1C3-UGTCs2 B0034-UGTCs2-His + 4. pSB1A3-Red -> 127. pSB1A3-B0034-UGTCs2-His Digest Collagen binding protein → cloned from strain 14.4 Cell surface protein → cloned from strain 3.1 usp45 → cloned from strain 13.1 1.1 pSB1C3-red (From strain database 2013) Transformation: 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His O/N & Screening: 75. pEL3A15 - CrtB 102. pSB1C3 - CrtE Plasmid Prep(Glycerol stock) 79. pEL3K16 - CrtE 101. pSB1C3 - DNA program123456 PCR 21. pSB4C5 - pBAD/AraC-CrtE~I /w RBS, ZF (E,P)(To remove promotor) 57.pSB1C3-B0034-Idi mutagenesis (Phusion) to remove restriction site. Gel-electrophoresis of screening PCR products: 111. pSBLb4C15 112. pSBLbEc4C15 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB4S15-CP6-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP Sequencing preparation: 62. pSB1C3-CP8 64. pSB1C3-CP29 65. pSB1C3-CP30 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP Plasmid preparation: 74. pSB3K3-CP44-B0032-BFP Gel-electrophoresis of overnight PCR products: Collagen binding protein → cloned from strain 14.4 Cell surface protein → cloned from strain 3.1 usp45 → cloned from strain 13.1 Re-streak: Lb18. Lb. delbrueckii bulgaricus no plasmid Spreading of strains: Lb19. Lc.thermophilus no plasmid* *With 1x, 10x & 50x dilution <h2>Results</~~h3> <br~~>57.pSB1C3-B0034-Idi worked, gel run showed a band at suspected size , but also some smudge, probably primer dimers. 101. pSB1C3 - DNA program123456 worked and sent for sequence Transformations: 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His all grew red and white colonies, indicating successful ligation and transformation. Gel-electrophoresis of overnight PCR products: Collagen binding protein → cloned from strain 14.4: NC checks good and lines are equal to ~200bp. Cell surface protein → cloned from strain 3.1: NC checks good and lines are equal to ~150bp. usp45 → cloned from strain 13.1: NC checks good and lines are equal to ~140bp. </div>';

+

ds = '<div id="dairy-text"> <h1>Thursday 2013-07-18</h1> Name of participants: Karl H, Ken B-A, Kristoffer L, Christoffer, Sabri, Alexander, Nils, Thorsteinn, Magnus, Malin, Pontus, Niclas <h2>Ongoing constructs:</h2>E.coli (strain D5α): 75. pEL3A15 - CrtB 99. pSB1C3 - CrtO 79. pEL3K16 - CrtE 102. pSB1C3 - CrtE 101. pSB1C3 - DNA program123456 96. Cs42s-Promoter-ZCD-PsbA2-UGTCs2-PsbA2-Beta-carotene hydroxylase-B1002 57.Psb1C3-B0034-Idi 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 7.pSB3k3-red 22. B0032-BFP 42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 100. pJP059 111. pSBLb4C15 112. pSBLbEc4C15 L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 Lb. delbrueckii bulgaricus: 18. Lb. delbrueckii bulgaricus Lc. thermophilus: 19. Lc.thermophilus <h2>Todays work</h2>Assembly 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL 120. pSB1C3-B0034-His-4CL Ligation 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL 120. pSB1C3-B0034-His-4CL Digest & Ligation: ZCD + 1. pSB1C3-Red -> 123. pSB1C3-ZCD ZCD-His + 1. pSB1C3-Red -> 125. pSB1C3-ZCD-His UGTCs2 + 1. pSB1C3-Red -> 126. pSB1C3-UGTCs2 B0034-UGTCs2-His + 4. pSB1A3-Red -> 127. pSB1A3-B0034-UGTCs2-His Digest Collagen binding protein → cloned from strain 14.4 Cell surface protein → cloned from strain 3.1 usp45 → cloned from strain 13.1 1.1 pSB1C3-red (From strain database 2013) Transformation: 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His O/N & Screening: 75. pEL3A15 - CrtB 102. pSB1C3 - CrtE Plasmid Prep(Glycerol stock) 79. pEL3K16 - CrtE 101. pSB1C3 - DNA program123456 PCR 21. pSB4C5 - pBAD/AraC-CrtE~I /w RBS, ZF (E,P)(To remove promotor) 57.pSB1C3-B0034-Idi mutagenesis (Phusion) to remove restriction site. Gel-electrophoresis of screening PCR products: 111. pSBLb4C15 112. pSBLbEc4C15 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB4S15-CP6-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP Sequencing preparation: 62. pSB1C3-CP8 64. pSB1C3-CP29 65. pSB1C3-CP30 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP Plasmid preparation: 74. pSB3K3-CP44-B0032-BFP Gel-electrophoresis of overnight PCR products: Collagen binding protein → cloned from strain 14.4 Cell surface protein → cloned from strain 3.1 usp45 → cloned from strain 13.1 Re-streak: Lb18. Lb. delbrueckii bulgaricus no plasmid Spreading of strains: Lb19. Lc.thermophilus no plasmid* *With 1x, 10x & 50x dilution <h2>Results</h2>57.pSB1C3-B0034-Idi worked, gel run showed a band at suspected size , but also some smudge, probably primer dimers. 101. pSB1C3 - DNA program123456 worked and sent for sequence Transformations: 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His all grew red and white colonies, indicating successful ligation and transformation. Gel-electrophoresis of overnight PCR products: Collagen binding protein → cloned from strain 14.4: NC checks good and lines are equal to ~200bp. Cell surface protein → cloned from strain 3.1: NC checks good and lines are equal to ~150bp. usp45 → cloned from strain 13.1: NC checks good and lines are equal to ~140bp. </div>';

}

else if(id == 'd2013716')

{

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ds = '<div id="dairy-text"> <h1>Tuesday 2013-07-16</h1> Name of participants: Karl H, Lovisa P, Theodor L, Ken B-A, Victor S, Hampus.E, Kristoffer L, Christoffer .F, Sabri. J, Alexander, Nils, Thorsteinn, Magnus, Malin, Pontus, Niclas, Viktor Blomkvist(VB), Alona Nyberg (AN), Christoffer Ahlström (CA) <h2>Ongoing constructs:</h3> E.coli (strain D5α): 7.pSB3k3-red 22. B0032-BFP 42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 100. pJP059 111. pSBLb4C15 112. pSBLbEc4C15 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 75. pEL3A15 - CrtB 99. pSB1C3 - CrtO 79. pEL3K16 - CrtE 102. pSB1C3 - CrtE 101. pSB1C3 - DNA program123456 96. Cs42s-Promoter-ZCD-PsbA2-UGTCs2-PsbA2-Beta-carotene hydroxylase-B1002 91. BBa_J61002 (amp)-J23106 94. pSB1C3-K206000 95. pSB1C3-K206001 11. pSB4A15-blue(?) 58. pSB1C3-IspA 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 21. pSB4C5 - pBAD/AraC-CrtE~I /w RBS, ZF 57. PSB1C3-B0034-Idi L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 <h2>Todays work</~~h3> <br~~>Assembly 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL Ligation: 48. pSB1C3-B0034-His-STS Gel on Digest: 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 104. pSB1C3-B0034-4CL-B0034-STS Restreak: 47. pSB1C3-B0034-STS 75. pEL3A15 - CrtB Screening: 111. pSBLb4C15 112. pSBLbEc4C15 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB4S15-CP6-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP Plasmid preparation: 74. pSB3K3-CP44-B0032-BFP 27. pEL3K16-red Overnight: 74. pSB3K3-CP44-B0032-BFP Touchdown PCR with Phusion polymerase (signal peptides): CA Collagen binding protein → cloned from strain 14.4 Cell surface protein → cloned from strain 3.1 usp45 → cloned from strain 13.1 Transformation: 99. pSB1C3 - CrtO O/N & Screening 79. pEL3K16 - CrtE 102. pSB1C3 - CrtE 101. pSB1C3 - DNA program123456 Gelelectrophoresis 21. pSB4C5 - pBAD/AraC-CrtE~I /w RBS, ZF 57. PSB1C3-B0034-Idi (on pcr product from overnight pcr with new protocol) PCR 57.PSB1C3-B0034-IDI (overnight Tuesday-Wednesday new protocol) <h2>Results</~~h3> <br~~>Plasmid preparation: 74. pSB3K3-CP44-B0032-BFP: Failed! → Re-do! Screening: 111. pSBLb4C15: Failed 112. pSBLbEc4C15: Failed <h2>Other experiments</~~h3><br~~>Primer stock preparation: CA * CSP_RF CBP_RF usp45_RF CBP F *Obtained a concentration of 5µM at a volume of 200µl. </div>';

+

ds = '<div id="dairy-text"> <h1>Tuesday 2013-07-16</h1> Name of participants: Karl H, Lovisa P, Theodor L, Ken B-A, Victor S, Hampus.E, Kristoffer L, Christoffer .F, Sabri. J, Alexander, Nils, Thorsteinn, Magnus, Malin, Pontus, Niclas, Viktor Blomkvist(VB), Alona Nyberg (AN), Christoffer Ahlström (CA) <h2>Ongoing constructs:</h2> E.coli (strain D5α): 7.pSB3k3-red 22. B0032-BFP 42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 100. pJP059 111. pSBLb4C15 112. pSBLbEc4C15 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 75. pEL3A15 - CrtB 99. pSB1C3 - CrtO 79. pEL3K16 - CrtE 102. pSB1C3 - CrtE 101. pSB1C3 - DNA program123456 96. Cs42s-Promoter-ZCD-PsbA2-UGTCs2-PsbA2-Beta-carotene hydroxylase-B1002 91. BBa_J61002 (amp)-J23106 94. pSB1C3-K206000 95. pSB1C3-K206001 11. pSB4A15-blue(?) 58. pSB1C3-IspA 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 21. pSB4C5 - pBAD/AraC-CrtE~I /w RBS, ZF 57. PSB1C3-B0034-Idi L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 <h2>Todays work</h2>Assembly 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL Ligation: 48. pSB1C3-B0034-His-STS Gel on Digest: 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 104. pSB1C3-B0034-4CL-B0034-STS Restreak: 47. pSB1C3-B0034-STS 75. pEL3A15 - CrtB Screening: 111. pSBLb4C15 112. pSBLbEc4C15 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB4S15-CP6-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP Plasmid preparation: 74. pSB3K3-CP44-B0032-BFP 27. pEL3K16-red Overnight: 74. pSB3K3-CP44-B0032-BFP Touchdown PCR with Phusion polymerase (signal peptides): CA Collagen binding protein → cloned from strain 14.4 Cell surface protein → cloned from strain 3.1 usp45 → cloned from strain 13.1 Transformation: 99. pSB1C3 - CrtO O/N & Screening 79. pEL3K16 - CrtE 102. pSB1C3 - CrtE 101. pSB1C3 - DNA program123456 Gelelectrophoresis 21. pSB4C5 - pBAD/AraC-CrtE~I /w RBS, ZF 57. PSB1C3-B0034-Idi (on pcr product from overnight pcr with new protocol) PCR 57.PSB1C3-B0034-IDI (overnight Tuesday-Wednesday new protocol) <h2>Results</h2>Plasmid preparation: 74. pSB3K3-CP44-B0032-BFP: Failed! → Re-do! Screening: 111. pSBLb4C15: Failed 112. pSBLbEc4C15: Failed <h2>Other experiments</h2>Primer stock preparation: CA * CSP_RF CBP_RF usp45_RF CBP F *Obtained a concentration of 5µM at a volume of 200µl. </div>';

}

else if(id == 'd2013715')

{

-

ds = '<div id="dairy-text"> <h1>Monday 2013-07-15</h1> Name of participants: Karl H, Lovisa P, Theodor L, Ken B-A, Victor S, Hampus.E, Christoffer, Sabri, Alexander, Nils, Thorsteinn, Magnus, Malin, Pontus, Niclas <h2>Ongoing constructs:</~~h3> <br~~>E.coli (strain D5α): 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 105. pSB3T5-J23101-B0034-TAL 104. pSB1C3-B0034-4CL-B0034-STS 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 79. pEL3K16 - CrtE 102. pSB1C3 - CrtE 75. pEL3A15 - CrtB 101. pSB1C3 - DNA program123456 76. pEL3K16-CrtI 78. pEL3C18-CrtY 99. pSB1C3 - CrtO 57.Psb1C3-B0034-Idi 91. BBa_J61002 (amp)-J23106 94. pSB1C3-K206000 95. pSB1C3-K206001 11. pSB4A15-blue(?) 58. pSB1C3-IspA 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 7.pSB3k3-red 22. B0032-BFP 42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 100. pJP059 111. pSBLb4C15 112. pSBLbEc4C15 L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 14. DSM 20016-pLUL631(B?) 22. Betacarotene-various plasmids L. plantarum: 5. 256-rifR-pAMβ1 10. 256-p256 <h2>Todays work</~~h3> <br~~>PCR Purification: 111. pSBLb4C15, digest 112. pSBLbEc4C15, digest Ligation: 111. pSBLb4C15 (MluI+NheI) + Ori from pJP059 (MluI+NheI) 112. pSBLbEc4C15: (MluI+ClaI) + Ori from pJP059 (MluI+ClaI) Transformation: 111. pSBLb4C15 112. pSBLbEc4C15 75. pEL3A15 - CrtB 99. pSB1c3 - CrtO 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL Overnight: 74. pSB3K3-CP44-B0032-BFP 101. pSB1C3 - DNA program123456 Fluorescence(BFP) experiment with UV: 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB4S15-CP6-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP Plasmid preparation: Lb10.1 plantarum 256 p256 Lb10.2 plantarum 256 p256 Lb10.3 plantarum 256 p256 Lb22.1 reuteri betacarotene (strain name?) various plasmids 44.5 pSB1C3-b0034-His-Tal 74. pSB3K3-CP44-B0032-BFP PCR: 48. pSB1C3-B0034-His-STS Gel electhrophoresis 48. pSB1C3-B0034-His-STS Assembly: 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL Re-streak: 79. pEL3K16 - CrtE 102. pSB1C3 - CrtE 75. pEL3A15 - CrtB PCR cloning of: CrtE~I /w RBS, Zinc finger (PCR is done to remove promoter). Template should be the following construct, digested (E,P) 21. pBAD/AraC-CrtE~I /w RBS, Zinc finger CrtE~Z /w RBS, Zinc finger (PCR is done to remove promoter). Template should be the following construct, digested (E,P) 20. pBAD/AraC-CrtE~I /w RBS, Zinc finger CrtE~(O)-Z /w RBS, Zinc finger (PCR is done to remove promoter). Template should be the following construct, digested (E,P) 19. pBAD/AraC-CrtE~I /w RBS, Zinc finger CrtZ (PCR is done to clone and biobrick CrtZ from the following template, 20. pBAD/AraC-CrtE~CrtZ /w RBS, Zinc Finger Mutagenesis 57. pSB1C3-B0034-Idi Sequence preperation 78.1 pEL3C18-CrtY (VF and VR) 78.3 pEL3C18-CrtY (VF and VR) Screening: 111. pSBLb4C15 112. pSBLbEc4C15 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB4S15-CP6-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 101. pSB1C3 - DNA program123456 Touchdown PCR with Phusion polymerase (signal peptides): CA Collagen binding protein → cloned from strain 14.4 Cell surface protein → cloned from strain 3.1 usp45 → cloned from strain 13.1 <h2>Results</~~h3> <br~~>PCR, Succes on: 48. pSB1C3-B0034-His-STS Transformation 45. pSB1C3-B0034-4CL, failed 46. pSB1C3-B0034-His-4CL, failed 104. pSB1C3-B0034-4CL-B0034-STS, failed 106. pSB3T5-J23110-B0034-TAL, failed 108. pSB4A15-J23106-B0034-IspA, failed 109. pSB4A15-K206000-B0034-IspA, failed 110. pSB4A15-K206001-B0034-IspA, failed 111. pSBLb4C15 112. pSBLbEc4C15 -(45,46,104,106) Probably due to weak competent cells -(111-112) No growth. Possible problems: Too short time for proper ligation, no purification before ligation. Another attempt will be madwith the ligation from yesterday and a new one with purified digests. Nanodrop measurement of 57.Psb1C3-B0034-Idi was 80 ng/microliter. Gel: 21 pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268, fail Fluorescence(BFP) experiment with UV: 68. pSB3K3-CP1-B0032-BFP : Glowing (68.2 68.4) 69. pSB3K3-CP8-B0032-BFP : Glowing (69.3 69.4) 70. pSB3K3-CP11-B0032-BFP : Glowing (70.3) 71. pSB3K3-CP29-B0032-BFP : Glowing (71.1 71.2) 72. pSB3K3-CP30-B0032-BFP : Glowing (72.1 72.2) 73. pSB3K3-CP41-B0032-BFP : Glowing (73.4) 74. pSB3K3-CP44-B0032-BFP : Glowing (74.1.1 74.1.2) 81. pSB4S15-CP41-B0032-BFP : Not glowing 83. pSB4S15-CP30-B0032-BFP : Not glowing 86. pSB4S15-CP6-B0032-BFP : Not glowing 88. pSB4S15-CP1-B0032-BFP : Not glowing 89. pSB4S15-CP44-B0032-BFP : Not glowing Plasmid preparation: Lb10.1 plantarum 256 p256: 124.7 ng/µl Lb10.2 plantarum 256 p256: 23.5 ng/µl Lb10.3 plantarum 256 p256: 270.9 ng/µl Lb22.1 reuteri betacarotene (strain name?) various plasmids: 51.4 ng/µl </div>';

+

ds = '<div id="dairy-text"> <h1>Monday 2013-07-15</h1> Name of participants: Karl H, Lovisa P, Theodor L, Ken B-A, Victor S, Hampus.E, Christoffer, Sabri, Alexander, Nils, Thorsteinn, Magnus, Malin, Pontus, Niclas <h2>Ongoing constructs:</h2>E.coli (strain D5α): 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 105. pSB3T5-J23101-B0034-TAL 104. pSB1C3-B0034-4CL-B0034-STS 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 79. pEL3K16 - CrtE 102. pSB1C3 - CrtE 75. pEL3A15 - CrtB 101. pSB1C3 - DNA program123456 76. pEL3K16-CrtI 78. pEL3C18-CrtY 99. pSB1C3 - CrtO 57.Psb1C3-B0034-Idi 91. BBa_J61002 (amp)-J23106 94. pSB1C3-K206000 95. pSB1C3-K206001 11. pSB4A15-blue(?) 58. pSB1C3-IspA 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 7.pSB3k3-red 22. B0032-BFP 42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 100. pJP059 111. pSBLb4C15 112. pSBLbEc4C15 L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 14. DSM 20016-pLUL631(B?) 22. Betacarotene-various plasmids L. plantarum: 5. 256-rifR-pAMβ1 10. 256-p256 <h2>Todays work</h2>PCR Purification: 111. pSBLb4C15, digest 112. pSBLbEc4C15, digest Ligation: 111. pSBLb4C15 (MluI+NheI) + Ori from pJP059 (MluI+NheI) 112. pSBLbEc4C15: (MluI+ClaI) + Ori from pJP059 (MluI+ClaI) Transformation: 111. pSBLb4C15 112. pSBLbEc4C15 75. pEL3A15 - CrtB 99. pSB1c3 - CrtO 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL Overnight: 74. pSB3K3-CP44-B0032-BFP 101. pSB1C3 - DNA program123456 Fluorescence(BFP) experiment with UV: 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB4S15-CP6-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP Plasmid preparation: Lb10.1 plantarum 256 p256 Lb10.2 plantarum 256 p256 Lb10.3 plantarum 256 p256 Lb22.1 reuteri betacarotene (strain name?) various plasmids 44.5 pSB1C3-b0034-His-Tal 74. pSB3K3-CP44-B0032-BFP PCR: 48. pSB1C3-B0034-His-STS Gel electhrophoresis 48. pSB1C3-B0034-His-STS Assembly: 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL Re-streak: 79. pEL3K16 - CrtE 102. pSB1C3 - CrtE 75. pEL3A15 - CrtB PCR cloning of: CrtE~I /w RBS, Zinc finger (PCR is done to remove promoter). Template should be the following construct, digested (E,P) 21. pBAD/AraC-CrtE~I /w RBS, Zinc finger CrtE~Z /w RBS, Zinc finger (PCR is done to remove promoter). Template should be the following construct, digested (E,P) 20. pBAD/AraC-CrtE~I /w RBS, Zinc finger CrtE~(O)-Z /w RBS, Zinc finger (PCR is done to remove promoter). Template should be the following construct, digested (E,P) 19. pBAD/AraC-CrtE~I /w RBS, Zinc finger CrtZ (PCR is done to clone and biobrick CrtZ from the following template, 20. pBAD/AraC-CrtE~CrtZ /w RBS, Zinc Finger Mutagenesis 57. pSB1C3-B0034-Idi Sequence preperation 78.1 pEL3C18-CrtY (VF and VR) 78.3 pEL3C18-CrtY (VF and VR) Screening: 111. pSBLb4C15 112. pSBLbEc4C15 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB4S15-CP6-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 101. pSB1C3 - DNA program123456 Touchdown PCR with Phusion polymerase (signal peptides): CA Collagen binding protein → cloned from strain 14.4 Cell surface protein → cloned from strain 3.1 usp45 → cloned from strain 13.1 <h2>Results</h2>PCR, Succes on: 48. pSB1C3-B0034-His-STS Transformation 45. pSB1C3-B0034-4CL, failed 46. pSB1C3-B0034-His-4CL, failed 104. pSB1C3-B0034-4CL-B0034-STS, failed 106. pSB3T5-J23110-B0034-TAL, failed 108. pSB4A15-J23106-B0034-IspA, failed 109. pSB4A15-K206000-B0034-IspA, failed 110. pSB4A15-K206001-B0034-IspA, failed 111. pSBLb4C15 112. pSBLbEc4C15 -(45,46,104,106) Probably due to weak competent cells -(111-112) No growth. Possible problems: Too short time for proper ligation, no purification before ligation. Another attempt will be madwith the ligation from yesterday and a new one with purified digests. Nanodrop measurement of 57.Psb1C3-B0034-Idi was 80 ng/microliter. Gel: 21 pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268, fail Fluorescence(BFP) experiment with UV: 68. pSB3K3-CP1-B0032-BFP : Glowing (68.2 68.4) 69. pSB3K3-CP8-B0032-BFP : Glowing (69.3 69.4) 70. pSB3K3-CP11-B0032-BFP : Glowing (70.3) 71. pSB3K3-CP29-B0032-BFP : Glowing (71.1 71.2) 72. pSB3K3-CP30-B0032-BFP : Glowing (72.1 72.2) 73. pSB3K3-CP41-B0032-BFP : Glowing (73.4) 74. pSB3K3-CP44-B0032-BFP : Glowing (74.1.1 74.1.2) 81. pSB4S15-CP41-B0032-BFP : Not glowing 83. pSB4S15-CP30-B0032-BFP : Not glowing 86. pSB4S15-CP6-B0032-BFP : Not glowing 88. pSB4S15-CP1-B0032-BFP : Not glowing 89. pSB4S15-CP44-B0032-BFP : Not glowing Plasmid preparation: Lb10.1 plantarum 256 p256: 124.7 ng/µl Lb10.2 plantarum 256 p256: 23.5 ng/µl Lb10.3 plantarum 256 p256: 270.9 ng/µl Lb22.1 reuteri betacarotene (strain name?) various plasmids: 51.4 ng/µl </div>';

}

else if(id == 'd201372')

Line 279:

else if(id == 'd201381')

{

-

ds = '<div id="dairy-text"><h1>Thursday 2013-08-01</h1>Name of participants: Marcus H, Karl H, Lovisa P, Emil M, Victor S, Hampus.E <h2>Ongoing constructs:</~~h3><br~~>' +

+

ds = '<div id="dairy-text"><h1>Thursday 2013-08-01</h1>Name of participants: Marcus H, Karl H, Lovisa P, Emil M, Victor S, Hampus.E <h2>Ongoing constructs:</h2>' +

'114. pSB1C3-B0034-TAL(MUT) 105. pSB3T5-J23101-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3K5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL '+

'137. pSB3K3-CP8-B0034-TAL 138. pSB3K3-CP8-B0034-His-TAL 139. pSB3K3-J23101-B0034-BFP 171. pSB3T5-J23110-B0034-4CL-B0034-STS 172. pSB3T5-CP1-B0034-TAL 185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS 186. pEL3S17-CP1-B0034-4CL-B0034-STS '+

'187. pEL3S17-CP8-B0034-4CL-B0034-STS 188. pEL3S17-J23101-B0034-4CL-B0034-STS 189. pSB3K3-J23101-B0034-TAL 204. pEL3S17-J23110-HIS-STS 205.pSBLB4C15-CP1-B0034-HIS-TAL 206.pSB1C3-J23101-B0034-TAL '+

-

'<h2>Todays work</~~h3><br~~>PCR screening and gel electrophorersis of construct: 185.(5-10) pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS '+

+

'<h2>Todays work</h2>PCR screening and gel electrophorersis of construct: 185.(5-10) pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS '+

-

'<h2>sequencing prep. of constructs:</h3> 185.9-185.10 '+

+

'<h2>sequencing prep. of constructs:</h2> 185.9-185.10 '+

-

'<h2>Transformation of constructs:</h3> 171. pSB3T5-J23110-B0034-4CL-B0034-STS 204. pEL3S17-J23110-HIS-STS 205.pSBLB4C15-CP1-B0034-HIS-TAL 206.pSB1C3-J23101-B0034-TAL '+

+

'<h2>Transformation of constructs:</h2> 171. pSB3T5-J23110-B0034-4CL-B0034-STS 204. pEL3S17-J23110-HIS-STS 205.pSBLB4C15-CP1-B0034-HIS-TAL 206.pSB1C3-J23101-B0034-TAL '+

-

'<h2>Results</~~h3><br~~>Construct 185, clone 9 and 10, bonds around 3000/4000 bp. '+

+

'<h2>Results</h2>Construct 185, clone 9 and 10, bonds around 3000/4000 bp. '+

-

'<h2>Follow up</h3> - '+

+

'<h2>Follow up</h2> - '+

-

'<h2>Other experiments</h3> - </div>';

+

'<h2>Other experiments</h2> - </div>';

}

else if(id == 'd2013919')

@@ Line 238: / Line 238: @@
    ds ='<p>på dig</p>';
   }
-    else if(id == 'd2013721')
+      else if(id == 'd2013721')
   {
 ds = '<div id="dairy-text"><h1>Sunday 2013-07-21</h1><p><strong>Name of participants:</strong>Hampus.E, Theodor L, Karl H, Ken B, Kristoffer L, Viktor @, Marcus H. Nils, Christoffer, Anders Edlund (AE)<br><br>'+
-'<h2>Ongoing constructs:</h3>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>121.pSB1C3-B0034-STS<br>121.pSB1C3-B0034-His-STS<br>'+
+'<h2>Ongoing constructs:</h2>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>121.pSB1C3-B0034-STS<br>121.pSB1C3-B0034-His-STS<br>'+
 '99. pSB1C3-CrtO<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br>129. pSB1C3-Idi (mut)<br>21. CrtE~I /w RBS, Zinc finger (PCR prod)<br>128. pSIM5-tet(Nissle)<br>130. pSB1C3-CrtE~I /w RBS, Zinc Finger<br>131. pSB4A15-J23101-CrtE~I /w RBS, Zinc Finger<br>132. pSB4A15-J23106-CrtE~I /w RBS, Zinc Finger<br>133. pSB4A15 -J23110-CrtE~I /w RBS, Zinc Finger<br>7.pSB3k3-red<br>22. B0032-BFP<br>42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>84. pSB4S15-CP6<br>85. pSB4S15-CP25<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>90. BBa_J61002-J23101<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>100. pJP059<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>136.1 pSB1C3-usp45<br>'+
 '<strong>L. reuteri:</strong><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>14. DSM 20016-pLUL631(B?)<br><br><strong>L. plantarum:</strong><br>5. 256-rifR-pAMβ1<br><br><b>Lb. delbrueckii bulgaricus:</b><br>18. Lb. delbrueckii bulgaricus<br><br><b>Lc. thermophilus:</b><br>19. Lc.thermophilus<br><br>'+
-'<h1>Todays work:</h1><br><b>Plasmid Prep on:</b><br>117.2  pSB3T5-CP1-B0034-His-TAL<br>119.2 pSB1C3-B0034-4CL<br>120.1 pSB1C3-B0034-HIS-4CL<br>104.2 and 104.5 pSB1C3-B0034-4CL-B0034-STS<br>106.5 and 106.6 pSB3T5-J23110-B0034-TAL<br>122.2 pSB1C3-B0034-His-STS;<br><br><b>Re-streak:</b><br>131.  pSB4A15-BBa-J61002-J23106-CrtE~I /w RBS, Zinc finger<br>132.  pSB4A15-BBa-J61002-J23110-CrtE~I /w RBS, Zinc finger<br>133.  pSB4A15-BBa-J61002-J23101-CrtE~I /w RBS, Zinc finger<br>134.1 pSB1C3-CBP<br>134.2 pSB1C3-CBP<br>134.3 pSB1C3-CBP<br>134.4 pSB1C3-CBP<br>136.1 pSB1C3-usp45<br>136.2 pSB1C3-usp45<br>136.3 pSB1C3-usp45<br>136.4 pSB1C3-usp45<br><br><b>Gel:</b><br>21. CrtE~I/w RBS,ZF<br><br><b>Overnight & screening:</b><br>99.  pSB1C3-CrtO<br>128.pSIM5-tet (E.Coli Nissle) Endast O/N<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br><br>'+
+'<h2>Todays work:</h2><b>Plasmid Prep on:</b><br>117.2  pSB3T5-CP1-B0034-His-TAL<br>119.2 pSB1C3-B0034-4CL<br>120.1 pSB1C3-B0034-HIS-4CL<br>104.2 and 104.5 pSB1C3-B0034-4CL-B0034-STS<br>106.5 and 106.6 pSB3T5-J23110-B0034-TAL<br>122.2 pSB1C3-B0034-His-STS;<br><br><b>Re-streak:</b><br>131.  pSB4A15-BBa-J61002-J23106-CrtE~I /w RBS, Zinc finger<br>132.  pSB4A15-BBa-J61002-J23110-CrtE~I /w RBS, Zinc finger<br>133.  pSB4A15-BBa-J61002-J23101-CrtE~I /w RBS, Zinc finger<br>134.1 pSB1C3-CBP<br>134.2 pSB1C3-CBP<br>134.3 pSB1C3-CBP<br>134.4 pSB1C3-CBP<br>136.1 pSB1C3-usp45<br>136.2 pSB1C3-usp45<br>136.3 pSB1C3-usp45<br>136.4 pSB1C3-usp45<br><br><b>Gel:</b><br>21. CrtE~I/w RBS,ZF<br><br><b>Overnight & screening:</b><br>99.  pSB1C3-CrtO<br>128.pSIM5-tet (E.Coli Nissle) Endast O/N<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br><br>'+
 '<b>Overnight:</b><br>84.1 pSB4S15-CP6<br>84.2 pSB4S15-CP6<br>86.1 pSB4S15-CP6-B0032-BFP<br>86.2 pSB4S15-CP6-B0032-BFP<br>87(1.1) pSB4S15-CP25-B0032-BFP<br>87(1.2) pSB4S15-CP25-B0032-BFP<br>88.1 pSB4S15-CP1-B0032-BFP<br>88.2 pSB4S15-CP1-B0032-BFP<br>89.1 pSB4S15-CP44-B0032-BFP<br>89.2 pSB4S15-CP44-B0032-BFP<br>97.1 pSB4S15-CP8-B0032-BFP<br>97.2 pSB4S15-CP8-B0032-BFP<br>98.1 pSB4S15-CP11-B0032-BFP<br>98.2 pSB4S15-CP11-B0032-BFP<br><br>'+
-'<h1>Results:</h1><br><b>Re-streak:</b><br>131.  pSB4A15-BBa-J61002-J23106-CrtE~I /w RBS, Zinc finger - Unable, no clones<br>132.  pSB4A15-BBa-J61002-J23110-CrtE~I /w RBS, Zinc finger - 3 clones restreaked<br>133.  pSB4A15-BBa-J61002-J23101-CrtE~I /w RBS, Zinc finger - 3 clones restreaked<br><br><b>Gel</b><br>21. CrtE~I/w RBS,ZF - fail<br><br><b>Overnight & screening:</b><br>99.  pSB1C3-CrtO<br>108. pSB4A15-J23106-B0034-IspA - Fail of all clones<br>109. pSB4A15-K206000-B0034-IspA - Fail of all clones<br>110. pSB4A15-K206001-B0034-IspA - Fail of all clones<br><br></div>';
+'<h2>Results:</h2><br><b>Re-streak:</b><br>131.  pSB4A15-BBa-J61002-J23106-CrtE~I /w RBS, Zinc finger - Unable, no clones<br>132.  pSB4A15-BBa-J61002-J23110-CrtE~I /w RBS, Zinc finger - 3 clones restreaked<br>133.  pSB4A15-BBa-J61002-J23101-CrtE~I /w RBS, Zinc finger - 3 clones restreaked<br><br><b>Gel</b><br>21. CrtE~I/w RBS,ZF - fail<br><br><b>Overnight & screening:</b><br>99.  pSB1C3-CrtO<br>108. pSB4A15-J23106-B0034-IspA - Fail of all clones<br>109. pSB4A15-K206000-B0034-IspA - Fail of all clones<br>110. pSB4A15-K206001-B0034-IspA - Fail of all clones<br><br></div>';
   }
   else if(id == 'd2013720')
   {
-ds = '<div id="dairy-text"><h1>Saturday 2013-07-20</h1><b>Name of participants:</b> Hampus.E, Kristoffer.L, Malin .B, Magnus, Nils, Pontus, Anders Edlund (AE), Stephanie Herman (SH)<br><br><h2>Ongoing constructs:</h3><br>99. pSB1C3-CrtO<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br>129. pSB1C3-Idi (mut)<br>21. CrtE~I /w RBS, Zinc finger (PCR prod)<br>128. pSIM5-tet(Nissle)<br>130. pSB1C3-CrtE~I /w RBS, Zinc Finger<br>131. pSB4A15-J23101-CrtE~I /w RBS, Zinc Finger<br>132. pSB4A15-J23106-CrtE~I /w RBS, Zinc Finger<br>133. pSB4A15 -J23110-CrtE~I /w RBS, Zinc Finger<br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>121.pSB1C3-B0034-STS<br>121.pSB1C3-B0034-His-STS<br>7.pSB3k3-red<br>22. B0032-BFP<br>42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>84. pSB4S15-CP6<br>85. pSB4S15-CP25<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>90. BBa_J61002-J23101<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>100. pJP059<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>136.1 pSB1C3-usp45<br><br><b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br><br><b>Lb. delbrueckii bulgaricus:</b><br>18. Lb. delbrueckii bulgaricus<br><br><b>Lc. thermophilus:</b><br>19. Lc.thermophilus<br><br><h2>Todays work</h3><br><br><b>Re-streak:</b><br>84. pSB4S15-CP6<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>99.  pSB1C3-CrtO<br><br><b>Overnigth:</b><br>136.1 pSB1C3-usp45<br>136.2 pSB1C3-usp45<br>111.6 pSBLb4C15-red<br>111.7 pSBLb4C15-red<br><br><b>Transformation: </b><br>131.  pSB4A15-BBa-J61002-J23106-CrtE~I /w RBS, Zinc finger <br>132.  pSB4A15-BBa-J61002-J23110-CrtE~I /w RBS, Zinc finger <br>133.  pSB4A15-BBa-J61002-J23101-CrtE~I /w RBS, Zinc finger<br>134. pSB1C3-CBP<br>135. pSB1C3-CSP<br>136. pSB1C3-USP45<br><br><b>Plasmidprep:</b><br>109. pSB4A15-K206000-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br>1.1? 90.3 j23101<br>1.2?<br>2.1?90.4 j23101<br>2.2?<br><br><b>Gel:</b><br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br><br><b>Screening pcr of constructs:</b><br>119.pSB1C3-B0034-4CL<br>120 pSB1C3-B0034-HIS-4CL<br>106 pSB3T5-J23110-B0034-TAL<br>117 pSB3T5-CP1-B0034-His-TAL<br>107 pSB3T5-CP1-B0034-TAL<br><br><b>PCR:</b><br>21. pSB4C5-pBAD/AraC-CrtE~I/w RBS, ZF (to remove promotor)<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br><br><h2>Results</h3><br><b>Overnight & screening:</b><br>99.  pSB1C3-CrtO<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br><br><b>Screening of: </b><br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br><i>showed negative results on all clones but 109.2. A new screening PCR of previously un-screened clones was prepared to run overnight.</i><b>Gel<br><br>Gel succes on: </b><br>106.5 pSB3T5-J23110-B0034-TAL<br>106.6 pSB3T5-J23110-B0034-TAL<br>117.1 pSB3T5-CP1-B0034-His-TAL<br>117.2 pSB3T5-CP1-B0034-His-TAL<br>119.2 pSB1C3-B0034-4CL<br><b>No band on:</b><br>107 pSB3T5-CP1-B0034-TAL<br>116 pSB3T5-J23110-B0034-His-TAL<br>120 pSB1C3-B0034-HIS-4CL<br></div>';
+ds = '<div id="dairy-text"><h1>Saturday 2013-07-20</h1><b>Name of participants:</b> Hampus.E, Kristoffer.L, Malin .B, Magnus, Nils, Pontus, Anders Edlund (AE), Stephanie Herman (SH)<br><br><h2>Ongoing constructs:</h2>99. pSB1C3-CrtO<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br>129. pSB1C3-Idi (mut)<br>21. CrtE~I /w RBS, Zinc finger (PCR prod)<br>128. pSIM5-tet(Nissle)<br>130. pSB1C3-CrtE~I /w RBS, Zinc Finger<br>131. pSB4A15-J23101-CrtE~I /w RBS, Zinc Finger<br>132. pSB4A15-J23106-CrtE~I /w RBS, Zinc Finger<br>133. pSB4A15 -J23110-CrtE~I /w RBS, Zinc Finger<br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>121.pSB1C3-B0034-STS<br>121.pSB1C3-B0034-His-STS<br>7.pSB3k3-red<br>22. B0032-BFP<br>42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>84. pSB4S15-CP6<br>85. pSB4S15-CP25<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>90. BBa_J61002-J23101<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>100. pJP059<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>136.1 pSB1C3-usp45<br><br><b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br><br><b>Lb. delbrueckii bulgaricus:</b><br>18. Lb. delbrueckii bulgaricus<br><br><b>Lc. thermophilus:</b><br>19. Lc.thermophilus<br><br><h2>Todays work</h2><b>Re-streak:</b><br>84. pSB4S15-CP6<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>99.  pSB1C3-CrtO<br><br><b>Overnigth:</b><br>136.1 pSB1C3-usp45<br>136.2 pSB1C3-usp45<br>111.6 pSBLb4C15-red<br>111.7 pSBLb4C15-red<br><br><b>Transformation: </b><br>131.  pSB4A15-BBa-J61002-J23106-CrtE~I /w RBS, Zinc finger <br>132.  pSB4A15-BBa-J61002-J23110-CrtE~I /w RBS, Zinc finger <br>133.  pSB4A15-BBa-J61002-J23101-CrtE~I /w RBS, Zinc finger<br>134. pSB1C3-CBP<br>135. pSB1C3-CSP<br>136. pSB1C3-USP45<br><br><b>Plasmidprep:</b><br>109. pSB4A15-K206000-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br>1.1? 90.3 j23101<br>1.2?<br>2.1?90.4 j23101<br>2.2?<br><br><b>Gel:</b><br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br><br><b>Screening pcr of constructs:</b><br>119.pSB1C3-B0034-4CL<br>120 pSB1C3-B0034-HIS-4CL<br>106 pSB3T5-J23110-B0034-TAL<br>117 pSB3T5-CP1-B0034-His-TAL<br>107 pSB3T5-CP1-B0034-TAL<br><br><b>PCR:</b><br>21. pSB4C5-pBAD/AraC-CrtE~I/w RBS, ZF (to remove promotor)<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br><br><h2>Results</h2><b>Overnight & screening:</b><br>99.  pSB1C3-CrtO<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br><br><b>Screening of: </b><br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br><i>showed negative results on all clones but 109.2. A new screening PCR of previously un-screened clones was prepared to run overnight.</i><b>Gel<br><br>Gel succes on: </b><br>106.5 pSB3T5-J23110-B0034-TAL<br>106.6 pSB3T5-J23110-B0034-TAL<br>117.1 pSB3T5-CP1-B0034-His-TAL<br>117.2 pSB3T5-CP1-B0034-His-TAL<br>119.2 pSB1C3-B0034-4CL<br><b>No band on:</b><br>107 pSB3T5-CP1-B0034-TAL<br>116 pSB3T5-J23110-B0034-His-TAL<br>120 pSB1C3-B0034-HIS-4CL<br></div>';
   }
    else if(id == 'd2013719')
   {
-ds = '<div id="dairy-text"><h1>Friday 2013-07-19</h1><br>Name of participants: Ken B-A, Kristoffer L, Malin, Magnus, Sabri, Nils, Pontus, <br>Christoffer Ahlström (CA), Stephanie Herman (SH) <br><br><h2>Ongoing constructs:</h3><br><b>E.coli (strain D5α): </b><br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br>129. pSB1C3-Idi (mut)<br>21. CrtE~I /w RBS, Zinc finger (PCR prod)<br>128. pSIM5-tet(Nissle)<br>130. pSB1C3-CrtE~I /w RBS, Zinc Finger<br>131. pSB4A15-J23101-CrtE~I /w RBS, Zinc Finger<br>132. pSB4A15-J23106-CrtE~I /w RBS, Zinc Finger<br>133. pSB4A15 -J23110-CrtE~I /w RBS, Zinc Finger<br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br> 107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>7.pSB3k3-red<br>42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>84. pSB4S15-CP6<br>85. pSB4S15-CP25<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>90. BBa_J61002-J23101<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>100. pJP059<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>136.1 pSB1C3-usp45<br><br><b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br><h2>Todays work</h3><br><b>Screening pcr of constructs:</b><br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>117. pSB3T5-CP1-B0034-His-Tal<br>119. pSB1C3-B0034-4CL (from 15)<br>120. pSB1C3-B0034-His-4CL (from 15)<br><br><b>Overnight & screening:</b><br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br><br><b>Plasmid preparation:</b><br>122.2. pSB1C3-B0034-His-STS (from 15)<br>90.3. BBa_J61002 (amp)-J23101<br>90.4. BBa_J61002 (amp)-J23101<br>104.2. pSB1C3-B0034-4CL-B0034-STS <br>104.5. pSB1C3-B0034-4CL-B0034-STS<br>111.2. pSBLb4C15-E.coli ori replaced with ori from lactobacillus (pJP059) in pEL4C15<br>111.3. pSBLb4C15-E.coli ori replaced with ori from lactobacillus (pJP059) in pEL4C15<br>111.4. pSBLb4C15-E.coli ori replaced with ori from lactobacillus (pJP059) in pEL4C15<br>121.1. pSB1C3-B0034-STS (from 15)<br>121.2. pSB1C3-B0034-STS (from 15)<br><br><b>Cloning:</b><br>21. CrtE~I /w RBS, Zinc finger → 1. pSB1C3-red<br><br><b>Assembly:</b><br>21. CrtE~I /w RBS, Zinc finger, 91. BBa-J61002-J23106 → 11. pSB4A15-red <br>21. CrtE~I /w RBS, Zinc finger, 92. BBa-J61002-J23110 → 11. pSB4A15-red <br>21. CrtE~I /w RBS, Zinc finger, 103. BBa-J61002-J23101 → 11. pSB4A15-red <br><br><<b>Gel electrophoresis:</b><br>129. pSB1C3-Idi (mut)<br><br><b>Restreak</b><br>128. pSIM5-tet(Nissle)<br><br><h2>Results:</h3><br><b>Screening: </b><br> 108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br><i>showed negative results on all clones but 109.2, which proceeded to overnight. A new screening PCR was prepared to run overnight.</i><br><br><i>showed positive results in all cases but 125.4 and 127.3. All other clones proceeded to overnight.</i><br><br><i>The overnight showed no contamination of negative controls, and growth in all clones but 127.1 and 127.2.</i><br><br><b>Transformation:</b><br>Only visual red colonies on CBP & CSP and possible 2 white colonies on usp45.<br>→ Re-do the transformation with the saved ligation solution from (18/07-13)<br>→ Re-streak the possible white colonies. <br><br></div>';
+ds = '<div id="dairy-text"><h1>Friday 2013-07-19</h1><br>Name of participants: Ken B-A, Kristoffer L, Malin, Magnus, Sabri, Nils, Pontus, <br>Christoffer Ahlström (CA), Stephanie Herman (SH) <br><br><h2>Ongoing constructs:</h2><b>E.coli (strain D5α): </b><br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br>129. pSB1C3-Idi (mut)<br>21. CrtE~I /w RBS, Zinc finger (PCR prod)<br>128. pSIM5-tet(Nissle)<br>130. pSB1C3-CrtE~I /w RBS, Zinc Finger<br>131. pSB4A15-J23101-CrtE~I /w RBS, Zinc Finger<br>132. pSB4A15-J23106-CrtE~I /w RBS, Zinc Finger<br>133. pSB4A15 -J23110-CrtE~I /w RBS, Zinc Finger<br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br> 107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>7.pSB3k3-red<br>42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>84. pSB4S15-CP6<br>85. pSB4S15-CP25<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>90. BBa_J61002-J23101<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>100. pJP059<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>136.1 pSB1C3-usp45<br><br><b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br><h2>Todays work</h2><b>Screening pcr of constructs:</b><br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>117. pSB3T5-CP1-B0034-His-Tal<br>119. pSB1C3-B0034-4CL (from 15)<br>120. pSB1C3-B0034-His-4CL (from 15)<br><br><b>Overnight & screening:</b><br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br><br><b>Plasmid preparation:</b><br>122.2. pSB1C3-B0034-His-STS (from 15)<br>90.3. BBa_J61002 (amp)-J23101<br>90.4. BBa_J61002 (amp)-J23101<br>104.2. pSB1C3-B0034-4CL-B0034-STS <br>104.5. pSB1C3-B0034-4CL-B0034-STS<br>111.2. pSBLb4C15-E.coli ori replaced with ori from lactobacillus (pJP059) in pEL4C15<br>111.3. pSBLb4C15-E.coli ori replaced with ori from lactobacillus (pJP059) in pEL4C15<br>111.4. pSBLb4C15-E.coli ori replaced with ori from lactobacillus (pJP059) in pEL4C15<br>121.1. pSB1C3-B0034-STS (from 15)<br>121.2. pSB1C3-B0034-STS (from 15)<br><br><b>Cloning:</b><br>21. CrtE~I /w RBS, Zinc finger → 1. pSB1C3-red<br><br><b>Assembly:</b><br>21. CrtE~I /w RBS, Zinc finger, 91. BBa-J61002-J23106 → 11. pSB4A15-red <br>21. CrtE~I /w RBS, Zinc finger, 92. BBa-J61002-J23110 → 11. pSB4A15-red <br>21. CrtE~I /w RBS, Zinc finger, 103. BBa-J61002-J23101 → 11. pSB4A15-red <br><br><<b>Gel electrophoresis:</b><br>129. pSB1C3-Idi (mut)<br><br><b>Restreak</b><br>128. pSIM5-tet(Nissle)<br><br><h2>Results:</h2><b>Screening: </b><br> 108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br><i>showed negative results on all clones but 109.2, which proceeded to overnight. A new screening PCR was prepared to run overnight.</i><br><br><i>showed positive results in all cases but 125.4 and 127.3. All other clones proceeded to overnight.</i><br><br><i>The overnight showed no contamination of negative controls, and growth in all clones but 127.1 and 127.2.</i><br><br><b>Transformation:</b><br>Only visual red colonies on CBP & CSP and possible 2 white colonies on usp45.<br>→ Re-do the transformation with the saved ligation solution from (18/07-13)<br>→ Re-streak the possible white colonies. <br><br></div>';
   }
    else if(id == 'd2013718')
   {
-ds = '<div id="dairy-text"><br><h1>Thursday 2013-07-18</h1><br><br><b>Name of participants:</b> Karl H, Ken B-A, Kristoffer L, Christoffer, Alexander, Magnus, Pontus,Sabri,StephanieHerman (SH), Alona Nyberg (AN), Christoffer Ahlström (CA), Viktor Blomkvist (VB)<br><br><br><h2>Ongoing constructs:</h3><br><br><b>E.coli (strain D5α):</b><br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>7.pSB3k3-red<br>22. B0032-BFP<br>42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>84. pSB4S15-CP6<br>85. pSB4S15-CP25<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>90. BBa_J61002-J23101<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>100. pJP059<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br>129. pSB1C3-Idi (mut)<br>113. E.coli Nissle-pSIM5-tet<br><br><b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br><br><b>Lb. delbrueckii bulgaricus:</b><br>18. Lb. delbrueckii bulgaricus<br><br><b>Lc. thermophilus:</b><br>19. Lc.thermophilus<br><br><h2>Todays work</h3><br><br><b>Assembly</b><br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL<br>120. pSB1C3-B0034-His-4CL<br><br><br>Ligation<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL<br>120. pSB1C3-B0034-His-4CL<br><br><b>Gel-electrophoresis of screening PCR products</b><br>42. pSB4S15-red<br><br><b>Digestion:</b><br>42. pSB4S15-red<br><br><b>Transformation:</b><br>81. pSB4S15-CP41-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>84. pSB4S15-CP6<br>85. pSB4S15-CP25<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br><br><b>Overnight:</b> <br>90. BBa_J61002-J23101<br><br><b>Spreading of strains:</b> <br>81. pSB4S15-CP41-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>84. pSB4S15-CP6<br>85. pSB4S15-CP25<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br><br><b>Re-circulization:</b><br>129. pSB1C3-Idi (mut)<br><br><b>Plasmid Prep (Glycerol stock):</b><br>75. pEL3A15 - CrtB (75.1, 75.2, 75.4)<br><br><b>Transformation:</b><br>99. pSB1C3 - Crto <br>129. pSB1C3-Idi (mut)<br>113. E.coli Nissle-pSIM5-tet<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br><br><b>Re-streak:</b><br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br><br><br><b>Ligation of strains from yesterdays digest:</b><br>Collagen binding protein → cloned from strain 14.4<br>Cell surface protein → cloned from strain 3.1<br>usp45 → cloned from strain 13.1<br><br>*Ligated to 1.1 pSB1C3-red (From strain database 2013)<br><br><b>Transformation:</b>*<br>Collagen binding protein<br>Cell surface protein <br>usp45 <br><br>*Performed with D5(alpha) competent cells. <br><br><b>Screening:</b><br>111. pSBLb4C15<br><br><b>Spreading of strains:</b><br>Collagen binding protein [D5(alpha)] *<br>Cell surface protein [D5(alpha)] *<br>usp45 [D5(alpha)] *<br>111. pSBLb4C15 **<br>112. pSBLbEc4C15 **<br><br>*With a 10x and 1x dilution. <br><br>** 2 plates of each with 1x dilution.<br><br><h2>Results</h3><br><br><b>Re-streaks:</b><br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br>were successful on all clones but 108.7.<br><br>Transformation of 129. pSB1C3-Idi (mut) did not grow.<br><br><b>Electrophoresis on digests:</b> <br>*The gel showed that the components (signal peptides and destination plasmid) had been cut and therefore have the digest succeeded. <br><br></div>';
+ds = '<div id="dairy-text"><br><h1>Thursday 2013-07-18</h1><br><br><b>Name of participants:</b> Karl H, Ken B-A, Kristoffer L, Christoffer, Alexander, Magnus, Pontus,Sabri,StephanieHerman (SH), Alona Nyberg (AN), Christoffer Ahlström (CA), Viktor Blomkvist (VB)<br><br><br><h2>Ongoing constructs:</h2><b>E.coli (strain D5α):</b><br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>7.pSB3k3-red<br>22. B0032-BFP<br>42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>84. pSB4S15-CP6<br>85. pSB4S15-CP25<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>90. BBa_J61002-J23101<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>100. pJP059<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br>129. pSB1C3-Idi (mut)<br>113. E.coli Nissle-pSIM5-tet<br><br><b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br><br><b>Lb. delbrueckii bulgaricus:</b><br>18. Lb. delbrueckii bulgaricus<br><br><b>Lc. thermophilus:</b><br>19. Lc.thermophilus<br><br><h2>Todays work</h2><b>Assembly</b><br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL<br>120. pSB1C3-B0034-His-4CL<br><br><br>Ligation<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL<br>120. pSB1C3-B0034-His-4CL<br><br><b>Gel-electrophoresis of screening PCR products</b><br>42. pSB4S15-red<br><br><b>Digestion:</b><br>42. pSB4S15-red<br><br><b>Transformation:</b><br>81. pSB4S15-CP41-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>84. pSB4S15-CP6<br>85. pSB4S15-CP25<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br><br><b>Overnight:</b> <br>90. BBa_J61002-J23101<br><br><b>Spreading of strains:</b> <br>81. pSB4S15-CP41-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>84. pSB4S15-CP6<br>85. pSB4S15-CP25<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br><br><b>Re-circulization:</b><br>129. pSB1C3-Idi (mut)<br><br><b>Plasmid Prep (Glycerol stock):</b><br>75. pEL3A15 - CrtB (75.1, 75.2, 75.4)<br><br><b>Transformation:</b><br>99. pSB1C3 - Crto <br>129. pSB1C3-Idi (mut)<br>113. E.coli Nissle-pSIM5-tet<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br><br><b>Re-streak:</b><br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br><br><br><b>Ligation of strains from yesterdays digest:</b><br>Collagen binding protein → cloned from strain 14.4<br>Cell surface protein → cloned from strain 3.1<br>usp45 → cloned from strain 13.1<br><br>*Ligated to 1.1 pSB1C3-red (From strain database 2013)<br><br><b>Transformation:</b>*<br>Collagen binding protein<br>Cell surface protein <br>usp45 <br><br>*Performed with D5(alpha) competent cells. <br><br><b>Screening:</b><br>111. pSBLb4C15<br><br><b>Spreading of strains:</b><br>Collagen binding protein [D5(alpha)] *<br>Cell surface protein [D5(alpha)] *<br>usp45 [D5(alpha)] *<br>111. pSBLb4C15 **<br>112. pSBLbEc4C15 **<br><br>*With a 10x and 1x dilution. <br><br>** 2 plates of each with 1x dilution.<br><br><h2>Results</h2><b>Re-streaks:</b><br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br>were successful on all clones but 108.7.<br><br>Transformation of 129. pSB1C3-Idi (mut) did not grow.<br><br><b>Electrophoresis on digests:</b> <br>*The gel showed that the components (signal peptides and destination plasmid) had been cut and therefore have the digest succeeded. <br><br></div>';
   }
     else if(id == 'd2013717')
   {
-ds = '<div id="dairy-text"><br><h1>Thursday 2013-07-18</h1><br><br><b>Name of participants:</b> Karl H, Ken B-A, Kristoffer L, Christoffer, Sabri, Alexander, Nils, Thorsteinn, Magnus, Malin, Pontus, Niclas<br><br><br><h2>Ongoing constructs:</h3><br><br><b>E.coli (strain D5α):</b><br>75. pEL3A15 - CrtB<br>99. pSB1C3 - CrtO<br>79. pEL3K16 - CrtE <br>102. pSB1C3 - CrtE<br>101. pSB1C3 - DNA program123456<br>96. Cs42s-Promoter-ZCD-PsbA2-UGTCs2-PsbA2-Beta-carotene hydroxylase-B1002<br>57.Psb1C3-B0034-Idi<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>7.pSB3k3-red<br>22. B0032-BFP<br>42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>100. pJP059<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br><br><b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br><br><b>Lb. delbrueckii bulgaricus:</b><br>18. Lb. delbrueckii bulgaricus<br><br><b>Lc. thermophilus:</b><br>19. Lc.thermophilus<br><br><br><h2>Todays work</h3><br><br><b>Assembly</b><br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL<br>120. pSB1C3-B0034-His-4CL<br><br><br><b>Ligation</b><br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL<br>120. pSB1C3-B0034-His-4CL<br><br><b>Digest & Ligation:</b><br>ZCD + 1. pSB1C3-Red -> 123. pSB1C3-ZCD<br>ZCD-His + 1. pSB1C3-Red -> 125. pSB1C3-ZCD-His<br>UGTCs2 + 1. pSB1C3-Red -> 126. pSB1C3-UGTCs2<br>B0034-UGTCs2-His + 4. pSB1A3-Red -> 127. pSB1A3-B0034-UGTCs2-His<br><br><b>Digest</b><br>Collagen binding protein → cloned from strain 14.4<br>Cell surface protein → cloned from strain 3.1<br>usp45 → cloned from strain 13.1<br>1.1 pSB1C3-red (From strain database 2013)<br><br><b>Transformation:</b><br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br><br><b>O/N & Screening:</b><br>75. pEL3A15 - CrtB<br>102. pSB1C3 - CrtE<br><br><b>Plasmid Prep(Glycerol stock)</b><br>79. pEL3K16 - CrtE <br>101. pSB1C3 - DNA program123456<br><br><b>PCR</b><br>21. pSB4C5 - pBAD/AraC-CrtE~I /w RBS, ZF (E,P)(To remove promotor)<br>57.pSB1C3-B0034-Idi mutagenesis (Phusion) to remove restriction site. <br><br><b>Gel-electrophoresis of screening PCR products:</b><br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB4S15-CP6-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br><br><b>Sequencing preparation:</b><br>62. pSB1C3-CP8<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br><br><b>Plasmid preparation: </b><br>74. pSB3K3-CP44-B0032-BFP <br><br>Gel-electrophoresis of overnight PCR products:<br>Collagen binding protein → cloned from strain 14.4<br>Cell surface protein → cloned from strain 3.1<br>usp45 → cloned from strain 13.1<br><br><b>Re-streak: </b><br>Lb18. Lb. delbrueckii bulgaricus no plasmid<br><br><b>Spreading of strains: </b><br>Lb19. Lc.thermophilus no plasmid*<br><br>*With 1x, 10x & 50x dilution<br><br><br><h2>Results</h3><br><br>57.pSB1C3-B0034-Idi worked, gel run showed a band at suspected size , but also some smudge, probably primer dimers.<br>101. pSB1C3 - DNA program123456 worked and sent for sequence<br><br><b>Transformations:</b><br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br>all grew red and white colonies, indicating successful ligation and transformation.<br><br><b>Gel-electrophoresis of overnight PCR products:</b><br>Collagen binding protein → cloned from strain 14.4: NC checks good and lines are equal to ~200bp.<br>Cell surface protein → cloned from strain 3.1: NC checks good and lines are equal to ~150bp.<br>usp45 → cloned from strain 13.1: NC checks good and lines are equal to ~140bp.<br><br></div>';
+ds = '<div id="dairy-text"><br><h1>Thursday 2013-07-18</h1><br><br><b>Name of participants:</b> Karl H, Ken B-A, Kristoffer L, Christoffer, Sabri, Alexander, Nils, Thorsteinn, Magnus, Malin, Pontus, Niclas<br><br><br><h2>Ongoing constructs:</h2><b>E.coli (strain D5α):</b><br>75. pEL3A15 - CrtB<br>99. pSB1C3 - CrtO<br>79. pEL3K16 - CrtE <br>102. pSB1C3 - CrtE<br>101. pSB1C3 - DNA program123456<br>96. Cs42s-Promoter-ZCD-PsbA2-UGTCs2-PsbA2-Beta-carotene hydroxylase-B1002<br>57.Psb1C3-B0034-Idi<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>7.pSB3k3-red<br>22. B0032-BFP<br>42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>100. pJP059<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br><br><b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br><br><b>Lb. delbrueckii bulgaricus:</b><br>18. Lb. delbrueckii bulgaricus<br><br><b>Lc. thermophilus:</b><br>19. Lc.thermophilus<br><br><br><h2>Todays work</h2><b>Assembly</b><br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL<br>120. pSB1C3-B0034-His-4CL<br><br><br><b>Ligation</b><br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL<br>120. pSB1C3-B0034-His-4CL<br><br><b>Digest & Ligation:</b><br>ZCD + 1. pSB1C3-Red -> 123. pSB1C3-ZCD<br>ZCD-His + 1. pSB1C3-Red -> 125. pSB1C3-ZCD-His<br>UGTCs2 + 1. pSB1C3-Red -> 126. pSB1C3-UGTCs2<br>B0034-UGTCs2-His + 4. pSB1A3-Red -> 127. pSB1A3-B0034-UGTCs2-His<br><br><b>Digest</b><br>Collagen binding protein → cloned from strain 14.4<br>Cell surface protein → cloned from strain 3.1<br>usp45 → cloned from strain 13.1<br>1.1 pSB1C3-red (From strain database 2013)<br><br><b>Transformation:</b><br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br><br><b>O/N & Screening:</b><br>75. pEL3A15 - CrtB<br>102. pSB1C3 - CrtE<br><br><b>Plasmid Prep(Glycerol stock)</b><br>79. pEL3K16 - CrtE <br>101. pSB1C3 - DNA program123456<br><br><b>PCR</b><br>21. pSB4C5 - pBAD/AraC-CrtE~I /w RBS, ZF (E,P)(To remove promotor)<br>57.pSB1C3-B0034-Idi mutagenesis (Phusion) to remove restriction site. <br><br><b>Gel-electrophoresis of screening PCR products:</b><br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB4S15-CP6-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br><br><b>Sequencing preparation:</b><br>62. pSB1C3-CP8<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br><br><b>Plasmid preparation: </b><br>74. pSB3K3-CP44-B0032-BFP <br><br>Gel-electrophoresis of overnight PCR products:<br>Collagen binding protein → cloned from strain 14.4<br>Cell surface protein → cloned from strain 3.1<br>usp45 → cloned from strain 13.1<br><br><b>Re-streak: </b><br>Lb18. Lb. delbrueckii bulgaricus no plasmid<br><br><b>Spreading of strains: </b><br>Lb19. Lc.thermophilus no plasmid*<br><br>*With 1x, 10x & 50x dilution<br><br><br><h2>Results</h2>57.pSB1C3-B0034-Idi worked, gel run showed a band at suspected size , but also some smudge, probably primer dimers.<br>101. pSB1C3 - DNA program123456 worked and sent for sequence<br><br><b>Transformations:</b><br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br>all grew red and white colonies, indicating successful ligation and transformation.<br><br><b>Gel-electrophoresis of overnight PCR products:</b><br>Collagen binding protein → cloned from strain 14.4: NC checks good and lines are equal to ~200bp.<br>Cell surface protein → cloned from strain 3.1: NC checks good and lines are equal to ~150bp.<br>usp45 → cloned from strain 13.1: NC checks good and lines are equal to ~140bp.<br><br></div>';
   }
     else if(id == 'd2013716')
   {
-ds = '<div id="dairy-text"><br><h1>Tuesday 2013-07-16</h1><br><br><b>Name of participants:</b> Karl H, Lovisa P, Theodor L, Ken B-A, Victor S, Hampus.E, Kristoffer L, Christoffer .F, Sabri. J, Alexander, Nils, Thorsteinn, Magnus, Malin, Pontus, Niclas, Viktor Blomkvist(VB), Alona Nyberg (AN), Christoffer Ahlström (CA)<br><br><h2>Ongoing constructs:</h3><br><br><b>E.coli (strain D5α):</b><br>7.pSB3k3-red<br>22. B0032-BFP<br>42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>100. pJP059<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>75. pEL3A15 - CrtB<br>99. pSB1C3 - CrtO<br>79. pEL3K16 - CrtE <br>102. pSB1C3 - CrtE<br>101. pSB1C3 - DNA program123456<br>96. Cs42s-Promoter-ZCD-PsbA2-UGTCs2-PsbA2-Beta-carotene hydroxylase-B1002<br>91. BBa_J61002 (amp)-J23106<br>94. pSB1C3-K206000<br>95. pSB1C3-K206001<br>11. pSB4A15-blue(?)<br>58. pSB1C3-IspA<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>21. pSB4C5 - pBAD/AraC-CrtE~I /w RBS, ZF<br>57. PSB1C3-B0034-Idi <br><br><b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br><br><h2>Todays work</h3><br><br><b>Assembly</b><br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br><br><b>Ligation:</b><br>48. pSB1C3-B0034-His-STS<br><br><b>Gel on Digest:</b><br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>104. pSB1C3-B0034-4CL-B0034-STS<br><br><b>Restreak:</b><br>47. pSB1C3-B0034-STS<br>75. pEL3A15 - CrtB<br><br><b>Screening:</b> <br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB4S15-CP6-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br><br><b>Plasmid preparation: </b><br>74. pSB3K3-CP44-B0032-BFP<br>27. pEL3K16-red<br><br><b>Overnight: </b><br>74. pSB3K3-CP44-B0032-BFP<br><br><i>Touchdown PCR with Phusion polymerase (signal peptides): CA<br>Collagen binding protein → cloned from strain 14.4<br>Cell surface protein → cloned from strain 3.1<br>usp45 → cloned from strain 13.1</i><br><br><b>Transformation:</b><br>99. pSB1C3 - CrtO<br><br><b>O/N & Screening</b><br>79. pEL3K16 - CrtE <br>102. pSB1C3 - CrtE<br>101. pSB1C3 - DNA program123456<br><br><b>Gelelectrophoresis</b><br>21. pSB4C5 - pBAD/AraC-CrtE~I /w RBS, ZF<br>57. PSB1C3-B0034-Idi (on pcr product from overnight pcr with new protocol) <br><br><b>PCR </b><br>57.PSB1C3-B0034-IDI (overnight Tuesday-Wednesday new protocol) <br><br><h2>Results</h3><br><br><b>Plasmid preparation: </b><br>74. pSB3K3-CP44-B0032-BFP: Failed! → Re-do!<br><br><b>Screening:</b> <br>111. pSBLb4C15: Failed<br>112. pSBLbEc4C15: Failed<br><br><h2>Other experiments</h3><br>Primer stock preparation: CA *<br>CSP_RF<br>CBP_RF<br>usp45_RF<br>CBP F<br>*Obtained a concentration of 5µM at a volume of 200µl.<br><br></div>';
+ds = '<div id="dairy-text"><br><h1>Tuesday 2013-07-16</h1><br><br><b>Name of participants:</b> Karl H, Lovisa P, Theodor L, Ken B-A, Victor S, Hampus.E, Kristoffer L, Christoffer .F, Sabri. J, Alexander, Nils, Thorsteinn, Magnus, Malin, Pontus, Niclas, Viktor Blomkvist(VB), Alona Nyberg (AN), Christoffer Ahlström (CA)<br><br><h2>Ongoing constructs:</h2><br><br><b>E.coli (strain D5α):</b><br>7.pSB3k3-red<br>22. B0032-BFP<br>42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>100. pJP059<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>75. pEL3A15 - CrtB<br>99. pSB1C3 - CrtO<br>79. pEL3K16 - CrtE <br>102. pSB1C3 - CrtE<br>101. pSB1C3 - DNA program123456<br>96. Cs42s-Promoter-ZCD-PsbA2-UGTCs2-PsbA2-Beta-carotene hydroxylase-B1002<br>91. BBa_J61002 (amp)-J23106<br>94. pSB1C3-K206000<br>95. pSB1C3-K206001<br>11. pSB4A15-blue(?)<br>58. pSB1C3-IspA<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>21. pSB4C5 - pBAD/AraC-CrtE~I /w RBS, ZF<br>57. PSB1C3-B0034-Idi <br><br><b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br><br><h2>Todays work</h2><b>Assembly</b><br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br><br><b>Ligation:</b><br>48. pSB1C3-B0034-His-STS<br><br><b>Gel on Digest:</b><br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>104. pSB1C3-B0034-4CL-B0034-STS<br><br><b>Restreak:</b><br>47. pSB1C3-B0034-STS<br>75. pEL3A15 - CrtB<br><br><b>Screening:</b> <br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB4S15-CP6-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br><br><b>Plasmid preparation: </b><br>74. pSB3K3-CP44-B0032-BFP<br>27. pEL3K16-red<br><br><b>Overnight: </b><br>74. pSB3K3-CP44-B0032-BFP<br><br><i>Touchdown PCR with Phusion polymerase (signal peptides): CA<br>Collagen binding protein → cloned from strain 14.4<br>Cell surface protein → cloned from strain 3.1<br>usp45 → cloned from strain 13.1</i><br><br><b>Transformation:</b><br>99. pSB1C3 - CrtO<br><br><b>O/N & Screening</b><br>79. pEL3K16 - CrtE <br>102. pSB1C3 - CrtE<br>101. pSB1C3 - DNA program123456<br><br><b>Gelelectrophoresis</b><br>21. pSB4C5 - pBAD/AraC-CrtE~I /w RBS, ZF<br>57. PSB1C3-B0034-Idi (on pcr product from overnight pcr with new protocol) <br><br><b>PCR </b><br>57.PSB1C3-B0034-IDI (overnight Tuesday-Wednesday new protocol) <br><br><h2>Results</h2><b>Plasmid preparation: </b><br>74. pSB3K3-CP44-B0032-BFP: Failed! → Re-do!<br><br><b>Screening:</b> <br>111. pSBLb4C15: Failed<br>112. pSBLbEc4C15: Failed<br><br><h2>Other experiments</h2>Primer stock preparation: CA *<br>CSP_RF<br>CBP_RF<br>usp45_RF<br>CBP F<br>*Obtained a concentration of 5µM at a volume of 200µl.<br><br></div>';
   }
   else if(id == 'd2013715')
   {
-ds = '<div id="dairy-text"><br><h1>Monday 2013-07-15</h1><br><br><b>Name of participants:</b> Karl H, Lovisa P, Theodor L, Ken B-A, Victor S, Hampus.E, Christoffer, Sabri, Alexander, Nils, Thorsteinn, Magnus, Malin, Pontus, Niclas<br><br><h2>Ongoing constructs:</h3><br><br><b>E.coli (strain D5α):</b><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>105. pSB3T5-J23101-B0034-TAL<br>104. pSB1C3-B0034-4CL-B0034-STS<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>79. pEL3K16 - CrtE <br>102. pSB1C3 - CrtE<br>75. pEL3A15 - CrtB<br>101. pSB1C3 - DNA program123456<br>76. pEL3K16-CrtI<br>78. pEL3C18-CrtY<br>99. pSB1C3 - CrtO<br>57.Psb1C3-B0034-Idi<br>91. BBa_J61002 (amp)-J23106<br>94. pSB1C3-K206000<br>95. pSB1C3-K206001<br>11. pSB4A15-blue(?)<br>58. pSB1C3-IspA<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>7.pSB3k3-red<br>22. B0032-BFP<br>42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>100. pJP059<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br><br><b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>14. DSM 20016-pLUL631(B?)<br>22. Betacarotene-various plasmids<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-p256<br><br><h2>Todays work</h3><br><br><b>PCR Purification:</b><br>111. pSBLb4C15, digest<br>112. pSBLbEc4C15, digest<br><br><b>Ligation: </b><br>111. pSBLb4C15 (MluI+NheI) + Ori from pJP059 (MluI+NheI) <br>112. pSBLbEc4C15: (MluI+ClaI) + Ori from pJP059 (MluI+ClaI)<br><br><b>Transformation: </b><br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>75. pEL3A15 - CrtB<br>99. pSB1c3 - CrtO<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br><br><b>Overnight: </b><br>74. pSB3K3-CP44-B0032-BFP<br>101. pSB1C3 - DNA program123456 <br><br><b>Fluorescence(BFP) experiment with UV: </b><br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB4S15-CP6-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br><br><b>Plasmid preparation: </b><br>Lb10.1 plantarum 256 p256<br>Lb10.2 plantarum 256 p256<br>Lb10.3 plantarum 256 p256<br>Lb22.1 reuteri betacarotene (strain name?) various plasmids<br>44.5 pSB1C3-b0034-His-Tal<br>74. pSB3K3-CP44-B0032-BFP<br><br><b>PCR:</b><br>48. pSB1C3-B0034-His-STS<br><br><b>Gel electhrophoresis</b><br>48. pSB1C3-B0034-His-STS<br><br><b>Assembly:</b><br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br><br><b>Re-streak:</b><br>79. pEL3K16 - CrtE <br>102. pSB1C3 - CrtE<br>75. pEL3A15 - CrtB<br><br><br><b>PCR cloning of:</b><br>CrtE~I /w RBS, Zinc finger (PCR is done to remove promoter). Template should be the following construct, digested (E,P) 21. pBAD/AraC-CrtE~I /w RBS, Zinc finger <br><br>CrtE~Z /w RBS, Zinc finger (PCR is done to remove promoter). Template should be the following construct, digested (E,P) 20. pBAD/AraC-CrtE~I /w RBS, Zinc finger <br><br>CrtE~(O)-Z /w RBS, Zinc finger (PCR is done to remove promoter). Template should be the following construct, digested (E,P) 19. pBAD/AraC-CrtE~I /w RBS, Zinc finger <br><br>CrtZ (PCR is done to clone and biobrick CrtZ from the following template, 20. pBAD/AraC-CrtE~CrtZ /w RBS, Zinc Finger<br><br><b>Mutagenesis</b> <br>57. pSB1C3-B0034-Idi <br><br><b>Sequence preperation</b><br>78.1 pEL3C18-CrtY (VF and VR)<br>78.3 pEL3C18-CrtY (VF and VR)<br><br><b>Screening:</b><br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB4S15-CP6-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>101. pSB1C3 - DNA program123456 <br><br><i>Touchdown PCR with Phusion polymerase (signal peptides): CA<br>Collagen binding protein → cloned from strain 14.4<br>Cell surface protein → cloned from strain 3.1<br>usp45 → cloned from strain 13.1</i><br><br><h2>Results</h3><br><br><b>PCR, Succes on:</b><br>48. pSB1C3-B0034-His-STS<br><br><b>Transformation </b><br>45. pSB1C3-B0034-4CL, failed<br>46. pSB1C3-B0034-His-4CL, failed<br>104. pSB1C3-B0034-4CL-B0034-STS, failed<br>106. pSB3T5-J23110-B0034-TAL, failed<br>108. pSB4A15-J23106-B0034-IspA, failed<br>109. pSB4A15-K206000-B0034-IspA, failed<br>110. pSB4A15-K206001-B0034-IspA, failed<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br><br><i>-(45,46,104,106) Probably due to weak competent cells<br>-(111-112) No growth. Possible problems: Too short time for proper ligation, no purification before ligation. Another attempt will be madwith the ligation from yesterday and a new one with purified digests.<br><br>Nanodrop measurement of 57.Psb1C3-B0034-Idi was 80 ng/microliter.</i><br><br><b>Gel:</b><br>21 pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268, fail<br><br><b>Fluorescence(BFP) experiment with UV:</b><br>68. pSB3K3-CP1-B0032-BFP : Glowing (68.2 68.4)<br>69. pSB3K3-CP8-B0032-BFP : Glowing (69.3 69.4)<br>70. pSB3K3-CP11-B0032-BFP : Glowing (70.3)<br>71. pSB3K3-CP29-B0032-BFP : Glowing (71.1 71.2)<br>72. pSB3K3-CP30-B0032-BFP : Glowing (72.1 72.2)<br>73. pSB3K3-CP41-B0032-BFP : Glowing (73.4)<br>74. pSB3K3-CP44-B0032-BFP : Glowing (74.1.1 74.1.2)<br>81. pSB4S15-CP41-B0032-BFP : Not glowing<br>83. pSB4S15-CP30-B0032-BFP : Not glowing<br>86. pSB4S15-CP6-B0032-BFP : Not glowing<br>88. pSB4S15-CP1-B0032-BFP : Not glowing<br>89. pSB4S15-CP44-B0032-BFP : Not glowing<br><br><b>Plasmid preparation:</b><br>Lb10.1 plantarum 256 p256: 					124.7 ng/µl<br>Lb10.2 plantarum 256 p256: 					23.5 ng/µl<br>Lb10.3 plantarum 256 p256: 					270.9 ng/µl <br>Lb22.1 reuteri betacarotene (strain name?) various plasmids: 51.4 ng/µl<br><br></div>';
+ds = '<div id="dairy-text"><br><h1>Monday 2013-07-15</h1><br><br><b>Name of participants:</b> Karl H, Lovisa P, Theodor L, Ken B-A, Victor S, Hampus.E, Christoffer, Sabri, Alexander, Nils, Thorsteinn, Magnus, Malin, Pontus, Niclas<br><br><h2>Ongoing constructs:</h2><b>E.coli (strain D5α):</b><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>105. pSB3T5-J23101-B0034-TAL<br>104. pSB1C3-B0034-4CL-B0034-STS<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>79. pEL3K16 - CrtE <br>102. pSB1C3 - CrtE<br>75. pEL3A15 - CrtB<br>101. pSB1C3 - DNA program123456<br>76. pEL3K16-CrtI<br>78. pEL3C18-CrtY<br>99. pSB1C3 - CrtO<br>57.Psb1C3-B0034-Idi<br>91. BBa_J61002 (amp)-J23106<br>94. pSB1C3-K206000<br>95. pSB1C3-K206001<br>11. pSB4A15-blue(?)<br>58. pSB1C3-IspA<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>7.pSB3k3-red<br>22. B0032-BFP<br>42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>100. pJP059<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br><br><b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>14. DSM 20016-pLUL631(B?)<br>22. Betacarotene-various plasmids<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-p256<br><br><h2>Todays work</h2><b>PCR Purification:</b><br>111. pSBLb4C15, digest<br>112. pSBLbEc4C15, digest<br><br><b>Ligation: </b><br>111. pSBLb4C15 (MluI+NheI) + Ori from pJP059 (MluI+NheI) <br>112. pSBLbEc4C15: (MluI+ClaI) + Ori from pJP059 (MluI+ClaI)<br><br><b>Transformation: </b><br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>75. pEL3A15 - CrtB<br>99. pSB1c3 - CrtO<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br><br><b>Overnight: </b><br>74. pSB3K3-CP44-B0032-BFP<br>101. pSB1C3 - DNA program123456 <br><br><b>Fluorescence(BFP) experiment with UV: </b><br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB4S15-CP6-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br><br><b>Plasmid preparation: </b><br>Lb10.1 plantarum 256 p256<br>Lb10.2 plantarum 256 p256<br>Lb10.3 plantarum 256 p256<br>Lb22.1 reuteri betacarotene (strain name?) various plasmids<br>44.5 pSB1C3-b0034-His-Tal<br>74. pSB3K3-CP44-B0032-BFP<br><br><b>PCR:</b><br>48. pSB1C3-B0034-His-STS<br><br><b>Gel electhrophoresis</b><br>48. pSB1C3-B0034-His-STS<br><br><b>Assembly:</b><br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br><br><b>Re-streak:</b><br>79. pEL3K16 - CrtE <br>102. pSB1C3 - CrtE<br>75. pEL3A15 - CrtB<br><br><br><b>PCR cloning of:</b><br>CrtE~I /w RBS, Zinc finger (PCR is done to remove promoter). Template should be the following construct, digested (E,P) 21. pBAD/AraC-CrtE~I /w RBS, Zinc finger <br><br>CrtE~Z /w RBS, Zinc finger (PCR is done to remove promoter). Template should be the following construct, digested (E,P) 20. pBAD/AraC-CrtE~I /w RBS, Zinc finger <br><br>CrtE~(O)-Z /w RBS, Zinc finger (PCR is done to remove promoter). Template should be the following construct, digested (E,P) 19. pBAD/AraC-CrtE~I /w RBS, Zinc finger <br><br>CrtZ (PCR is done to clone and biobrick CrtZ from the following template, 20. pBAD/AraC-CrtE~CrtZ /w RBS, Zinc Finger<br><br><b>Mutagenesis</b> <br>57. pSB1C3-B0034-Idi <br><br><b>Sequence preperation</b><br>78.1 pEL3C18-CrtY (VF and VR)<br>78.3 pEL3C18-CrtY (VF and VR)<br><br><b>Screening:</b><br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB4S15-CP6-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>101. pSB1C3 - DNA program123456 <br><br><i>Touchdown PCR with Phusion polymerase (signal peptides): CA<br>Collagen binding protein → cloned from strain 14.4<br>Cell surface protein → cloned from strain 3.1<br>usp45 → cloned from strain 13.1</i><br><br><h2>Results</h2><b>PCR, Succes on:</b><br>48. pSB1C3-B0034-His-STS<br><br><b>Transformation </b><br>45. pSB1C3-B0034-4CL, failed<br>46. pSB1C3-B0034-His-4CL, failed<br>104. pSB1C3-B0034-4CL-B0034-STS, failed<br>106. pSB3T5-J23110-B0034-TAL, failed<br>108. pSB4A15-J23106-B0034-IspA, failed<br>109. pSB4A15-K206000-B0034-IspA, failed<br>110. pSB4A15-K206001-B0034-IspA, failed<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br><br><i>-(45,46,104,106) Probably due to weak competent cells<br>-(111-112) No growth. Possible problems: Too short time for proper ligation, no purification before ligation. Another attempt will be madwith the ligation from yesterday and a new one with purified digests.<br><br>Nanodrop measurement of 57.Psb1C3-B0034-Idi was 80 ng/microliter.</i><br><br><b>Gel:</b><br>21 pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268, fail<br><br><b>Fluorescence(BFP) experiment with UV:</b><br>68. pSB3K3-CP1-B0032-BFP : Glowing (68.2 68.4)<br>69. pSB3K3-CP8-B0032-BFP : Glowing (69.3 69.4)<br>70. pSB3K3-CP11-B0032-BFP : Glowing (70.3)<br>71. pSB3K3-CP29-B0032-BFP : Glowing (71.1 71.2)<br>72. pSB3K3-CP30-B0032-BFP : Glowing (72.1 72.2)<br>73. pSB3K3-CP41-B0032-BFP : Glowing (73.4)<br>74. pSB3K3-CP44-B0032-BFP : Glowing (74.1.1 74.1.2)<br>81. pSB4S15-CP41-B0032-BFP : Not glowing<br>83. pSB4S15-CP30-B0032-BFP : Not glowing<br>86. pSB4S15-CP6-B0032-BFP : Not glowing<br>88. pSB4S15-CP1-B0032-BFP : Not glowing<br>89. pSB4S15-CP44-B0032-BFP : Not glowing<br><br><b>Plasmid preparation:</b><br>Lb10.1 plantarum 256 p256: 					124.7 ng/µl<br>Lb10.2 plantarum 256 p256: 					23.5 ng/µl<br>Lb10.3 plantarum 256 p256: 					270.9 ng/µl <br>Lb22.1 reuteri betacarotene (strain name?) various plasmids: 51.4 ng/µl<br><br></div>';
   }
    else if(id == 'd201372')
@@ Line 279: / Line 279: @@
 else if(id == 'd201381')
   {
-   ds = '<div id="dairy-text"><h1>Thursday 2013-08-01</h1><p>Name of participants: Marcus H, Karl H, Lovisa P, Emil M, Victor S, Hampus.E<br><br><h2>Ongoing constructs:</h3><br>' +
+   ds = '<div id="dairy-text"><h1>Thursday 2013-08-01</h1><p>Name of participants: Marcus H, Karl H, Lovisa P, Emil M, Victor S, Hampus.E<br><br><h2>Ongoing constructs:</h2>' +
    '114. pSB1C3-B0034-TAL(MUT)<br>105. pSB3T5-J23101-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3K5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>'+
    '137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>172. pSB3T5-CP1-B0034-TAL<br>185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>186. pEL3S17-CP1-B0034-4CL-B0034-STS<br>'+
    '187. pEL3S17-CP8-B0034-4CL-B0034-STS<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS<br>189. pSB3K3-J23101-B0034-TAL<br>204. pEL3S17-J23110-HIS-STS<br>205.pSBLB4C15-CP1-B0034-HIS-TAL<br>206.pSB1C3-J23101-B0034-TAL<br>'+
-   '<h2>Todays work</h3><br><b>PCR screening and gel electrophorersis of construct:</b><br>185.(5-10) pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>'+
+   '<h2>Todays work</h2><b>PCR screening and gel electrophorersis of construct:</b><br>185.(5-10) pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>'+
-   '<h2>sequencing prep. of constructs:</h3><br>185.9-185.10<br>'+
+   '<h2>sequencing prep. of constructs:</h2><br>185.9-185.10<br>'+
-   '<h2>Transformation of constructs:</h3><br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>204. pEL3S17-J23110-HIS-STS<br>205.pSBLB4C15-CP1-B0034-HIS-TAL<br>206.pSB1C3-J23101-B0034-TAL<br><br>'+
+   '<h2>Transformation of constructs:</h2><br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>204. pEL3S17-J23110-HIS-STS<br>205.pSBLB4C15-CP1-B0034-HIS-TAL<br>206.pSB1C3-J23101-B0034-TAL<br><br>'+
-   '<h2>Results</h3><br>Construct 185, clone 9 and 10, bonds around 3000/4000 bp.<br><br>'+
+   '<h2>Results</h2>Construct 185, clone 9 and 10, bonds around 3000/4000 bp.<br><br>'+
-   '<h2>Follow up</h3><br>- <br><br>'+
+   '<h2>Follow up</h2><br>- <br><br>'+
-   '<h2>Other experiments</h3><br>-<br></p></div>';
+   '<h2>Other experiments</h2><br>-<br></p></div>';
   }
   else if(id == 'd2013919')

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