June

Week 1

Monday

Tuesday

Preparation of freezable competent cells (M15): 40 alicos of 100µL
Test of the competent cells.

Wednesday

Begining of ‘intra-cell ROS concentration’ modelisation.
Transfection of competent cells with the biobricks :
- pLac
- mCherry
- cl Inverter
- RBS (Elowitz 1999)
- GFP
First contact with Arduino : the electronic device that will be used as a controler, just as the Cambridge 2010 iGEM team.
Culture of the BBs k174000 and K592004/5/6

Thursday

Friday

Team meeting

Week 2

Little Summary with Keywords

Monday

Construction of the pQE30::KillerRed vector

Incubated a 50mL overnight culture of E. coli M15[pRep4] cells (Qiagen, Venlo, Netherlands), containing the pQE30::αSNAP vector, in preparation for midiprep.

Tuesday

Construction of the pQE30::KillerRed vector

Midi-prepped pQE30::αSNAP. The DNA sample concentration was only of 10 ng/µL. The experiment has to be re performed.

Incubated a 10mL overnight culture of E. coli M15[pRep4-pQE30::αSNAP] cells, in preparation for miniprep.

Wednesday

Construction of the pQE30::KillerRed vector

Mini-prepped pQE30::αSNAP/pRep4 and got a 98,9 ng/µL DNA sample.

Digested pQE30::αSNAP with BamHI and KpnI restriction enzymes. Separation of the gene of non interest (αSNAP) from the pQE30 vector backbone by gel electrophoresis (1.2 % agarose, 30 min, 135V).

Thursday

Friday

Week 3

Week 4