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Team:Grenoble-EMSE-LSU/Documentation/Notebook/June
From 2013.igem.org
(Difference between revisions)
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Same transfections than wednesday, with fresh antibiotics.</br> | Same transfections than wednesday, with fresh antibiotics.</br> | ||
</br> | </br> | ||
| - | + | PCRs of pAraBad, mCherry and KillerRed, only pAraBAD worked.</br></br> | |
<strong>Team meeting</strong></br> | <strong>Team meeting</strong></br> | ||
TODO List :</br> | TODO List :</br> | ||
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<h3>Monday</h3> | <h3>Monday</h3> | ||
| + | <p>PCRs of mCherry and KR (did not work). Is there a problem with the primers or with the DNA templates? Need to work on that</br> | ||
| + | Put the transformed cells on petri dishes with the right antibiotics</br> /*what transformed cells*/ | ||
| + | Receive the photodiodes</br> | ||
| + | Begin the test of Arduino (digital output) by turning on and off a LED and using a Graphical User Interface (GUI) to control the intensity</br> | ||
| + | <p> | ||
<h4>Construction of the pQE30::KillerRed vector</h4> | <h4>Construction of the pQE30::KillerRed vector</h4> | ||
<p> | <p> | ||
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<h3>Wednesday</h3> | <h3>Wednesday</h3> | ||
| - | < | + | |
| - | < | + | <p><strong>Construction of the pQE30::KillerRed vector</strong></br> |
Mini-prepped pQE30::αSNAP/pRep4 and got a 98,9 ng/µL DNA sample.<br/> <br/> | Mini-prepped pQE30::αSNAP/pRep4 and got a 98,9 ng/µL DNA sample.<br/> <br/> | ||
Digested pQE30::αSNAP with BamHI and KpnI restriction enzymes. Separation of the gene of non interest (αSNAP) from the pQE30 vector backbone by gel electrophoresis (1.2 % agarose, 30 min, 135V).<br/> <br/> | Digested pQE30::αSNAP with BamHI and KpnI restriction enzymes. Separation of the gene of non interest (αSNAP) from the pQE30 vector backbone by gel electrophoresis (1.2 % agarose, 30 min, 135V).<br/> <br/> | ||
Revision as of 18:19, 18 September 2013
