Template:Kyoto/Notebook/Aug 27

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(Difference between revisions)
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|0.6&micro;L||0&micro;L||0&micro;L||1&micro;L||0.1&micro;L||8.3&micro;L||10&micro;L
|0.6&micro;L||0&micro;L||0&micro;L||1&micro;L||0.1&micro;L||8.3&micro;L||10&micro;L
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!8/17 DT-1(188&micro;g/mL)||EcoRI||XbaI||10xBufferB||100xBSA||MilliQ||total
!8/17 DT-1(188&micro;g/mL)||EcoRI||XbaI||10xBufferB||100xBSA||MilliQ||total

Revision as of 03:17, 19 September 2013

Aug 27

Restriction Enzyme Digestion

No name

8/21 pT181 attenuator-1(330µg/mL)EcoRISpeI10xBufferB100xBSAMilliQtotal
3.0µL0.5µL0.5µL3.0µL0.3µL22.7µL30µL
0.6µL0µL0µL1µL0.1µL8.3µL10µL
8/17 DT-1(188µg/mL)EcoRIXbaI10xBufferB100xBSAMilliQtotal
3.0µL0.5µL0.5µL3.0µL0.3µL22.7µL30µL
1.0µl0µL0µL1µL0.1µL7.9µL10µL
8/20 Pcon-RBS-luxR-DT-2(344µg/mL)EcoRISpeI10xBufferB100xBSAMilliQtotal
2.9µL0.5µL0.5µL3.0µL0.3µL22.8µL30µL
0.6µl0µL0µL1µL0.1µL8.3µL10µL
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