Template:Kyoto/Notebook/Aug 27

From 2013.igem.org

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|8||100bp ladder||--||--
|8||100bp ladder||--||--
|}
|}
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===Colony PCR===
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<!-- ここから -->
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<div class="experiment">
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<span class="author">Kojima and Yoshida</span>
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{| class="wikitable"
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!Sample||base pair
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|-
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|8/26 Pλ-luxI(1)||
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|-
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|8/26 Pλ-luxI(2)||
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|-
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|NC||
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|}
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{| class="wikitable"
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!PreDenature||Denature||Annealing||Extension||cycle
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|-
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|94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
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|-
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|5min||30s||30s||3min||30cycles
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|}
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</div>
<!-- ここまでをコピーしてね -->
<!-- ここまでをコピーしてね -->

Revision as of 04:31, 19 September 2013

Contents

Aug 27

Restriction Enzyme Digestion

No name

8/21 pT181 attenuator-1(330µg/mL)EcoRISpeI10x BufferB100x BSAMilliQtotal
3.0µL0.5µL0.5µL3.0µL0.3µL22.7µL30µL
0.6µL0µL0µL1µL0.1µL8.3µL10µL
8/17 DT-1(188µg/mL)EcoRIXbaI10x BufferB100x BSAMilliQtotal
3.0µL0.5µL0.5µL3.0µL0.3µL22.7µL30µL
1.0µl0µL0µL1µL0.1µL7.9µL10µL
8/20 Pcon-RBS-luxR-DT-2(344µg/mL)EcoRISpeI10x BufferB100x BSAMilliQtotal
2.9µL0.5µL0.5µL3.0µL0.3µL22.8µL30µL
0.6µl0µL0µL1µL0.1µL8.3µL10µL

Electrophoresis

No name

LaneSampleEnzyme1Enzyme2
1100bp ladder----
28/21 pT181 attenuatorEcoISpeI
38/21 pT181 atteniator----
48/17 DT-1EcoRIXbaI
58/17 DT-1----
68/20 Pcon-RBS-luxR-DT-2EcoISpeI
78/20 Pcon-RBS-luxR-DT-2----
8100bp ladder----

Colony PCR

Kojima and Yoshida

Samplebase pair
8/26 Pλ-luxI(1)
8/26 Pλ-luxI(2)
NC
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s3min30cycles
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