Template:Kyoto/Notebook/Aug 27

From 2013.igem.org

(Difference between revisions)
(→Aug 27)
(→Aug 27)
Line 67: Line 67:
|-
|-
|5min||30s||30s||3min||30cycles
|5min||30s||30s||3min||30cycles
 +
|}
 +
</div>
 +
===Liquid Culture===
 +
<!-- ここから -->
 +
<div class="experiment">
 +
<span class="author">No name</span>
 +
{| class="wikitable"
 +
!Sample||medium
 +
|-
 +
|8/26 Pλ-RBS-luxI-DT-1||Plusgrow(+Amp)
 +
|-
 +
|8/26 Pλ-RBS-luxI-DT-2||Plusgrow(+Amp)　
|}
|}
</div>
</div>
<!-- ここまでをコピーしてね -->
<!-- ここまでをコピーしてね -->

Revision as of 04:39, 19 September 2013

Contents

Aug 27

Restriction Enzyme Digestion

No name

8/21 pT181 attenuator-1(330µg/mL)EcoRISpeI10x BufferB100x BSAMilliQtotal
3.0µL0.5µL0.5µL3.0µL0.3µL22.7µL30µL
0.6µL0µL0µL1µL0.1µL8.3µL10µL
8/17 DT-1(188µg/mL)EcoRIXbaI10x BufferB100x BSAMilliQtotal
3.0µL0.5µL0.5µL3.0µL0.3µL22.7µL30µL
1.0µl0µL0µL1µL0.1µL7.9µL10µL
8/20 Pcon-RBS-luxR-DT-2(344µg/mL)EcoRISpeI10x BufferB100x BSAMilliQtotal
2.9µL0.5µL0.5µL3.0µL0.3µL22.8µL30µL
0.6µl0µL0µL1µL0.1µL8.3µL10µL

Electrophoresis

No name

LaneSampleEnzyme1Enzyme2
1100bp ladder----
28/21 pT181 attenuatorEcoISpeI
38/21 pT181 atteniator----
48/17 DT-1EcoRIXbaI
58/17 DT-1----
68/20 Pcon-RBS-luxR-DT-2EcoISpeI
78/20 Pcon-RBS-luxR-DT-2----
8100bp ladder----

Colony PCR

Kojima and Yoshida

Samplebase pair
8/26 Pλ-luxI(1)
8/26 Pλ-luxI(2)
NC
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s3min30cycles

Liquid Culture

No name

Samplemedium
8/26 Pλ-RBS-luxI-DT-1Plusgrow(+Amp)
8/26 Pλ-RBS-luxI-DT-2Plusgrow(+Amp)　
Retrieved from "http://2013.igem.org/Template:Kyoto/Notebook/Aug_27"