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Team:Hong Kong HKUST/notebook/mod2
From 2013.igem.org
(Difference between revisions)
| Line 301: | Line 301: | ||
<a href='#' class='head'>Week 4</a> | <a href='#' class='head'>Week 4</a> | ||
<div class='content' align="left"> | <div class='content' align="left"> | ||
| - | <br>June 24 <br> | + | <br><b>June 24 </b><br> |
Wet lab <br> | Wet lab <br> | ||
· Inoculation of pBlueScript KS(+) for training <br> | · Inoculation of pBlueScript KS(+) for training <br> | ||
| Line 310: | Line 310: | ||
Protocols review <br> | Protocols review <br> | ||
<br> | <br> | ||
| - | June 25 <br> | + | <b>June 25</b> <br> |
Wet lab <br> | Wet lab <br> | ||
· Plasmid extraction of pBlueScript KS(+) <br> | · Plasmid extraction of pBlueScript KS(+) <br> | ||
| Line 318: | Line 318: | ||
· Informal meeting <br> | · Informal meeting <br> | ||
<br> | <br> | ||
| - | June 26 <br> | + | <b>June 26</b> <br> |
Wet lab <br> | Wet lab <br> | ||
· Extraction of gDNA from HepG2 Cells for FABP1 and PPARa <br> | · Extraction of gDNA from HepG2 Cells for FABP1 and PPARa <br> | ||
| Line 324: | Line 324: | ||
· Transformation of BBa_K817002 (pFadBA) <br> | · Transformation of BBa_K817002 (pFadBA) <br> | ||
<br> | <br> | ||
| - | June 27 <br> | + | <b>June 27</b> <br> |
Wet lab <br> | Wet lab <br> | ||
· Inoculation of pEGFP-N1 for plasmid extraction <br> | · Inoculation of pEGFP-N1 for plasmid extraction <br> | ||
· Inoculation of BBa_K817002 (pFadBA) for plasmid extraction <br> | · Inoculation of BBa_K817002 (pFadBA) for plasmid extraction <br> | ||
<br> | <br> | ||
| - | June 28 <br> | + | <b>June 28 </b><br> |
Wet lab <br> | Wet lab <br> | ||
· Extraction of pEGFP-N1 plasmids by miniprep <br> | · Extraction of pEGFP-N1 plasmids by miniprep <br> | ||
| Line 338: | Line 338: | ||
· Ran 2% gel for BBa_K817002 (pFadBA) <br> | · Ran 2% gel for BBa_K817002 (pFadBA) <br> | ||
<br> | <br> | ||
| - | June 30 <br> | + | <b>June 30</b> <br> |
Dry lab <br> | Dry lab <br> | ||
Experiment planning and protocols revision for next week work<br></br> | Experiment planning and protocols revision for next week work<br></br> | ||
Revision as of 14:39, 19 September 2013
June 2013
Week 4
June 24
Wet lab
· Inoculation of pBlueScript KS(+) for training
· Autoclave basic materials
· Preparing LB
· LB-Ampicillin plates poured Dry lab
Protocols review
June 25
Wet lab
· Plasmid extraction of pBlueScript KS(+)
· LB-Chloramphenicol plates poured
Dry lab
· Primers design for FABP1, PPARa, GRP78
· Informal meeting
June 26
Wet lab
· Extraction of gDNA from HepG2 Cells for FABP1 and PPARa
· Transformation of pEGFP-N1
· Transformation of BBa_K817002 (pFadBA)
June 27
Wet lab
· Inoculation of pEGFP-N1 for plasmid extraction
· Inoculation of BBa_K817002 (pFadBA) for plasmid extraction
June 28
Wet lab
· Extraction of pEGFP-N1 plasmids by miniprep
· Extraction of BBa_K817002 (pFadBA)
· Restriction of pEGFP-N1 Restriction digestion by Ase1 and bamH1 for PPARa; Ase1 and Xho1 for GRP78, Xba1 and BamH1 for FABP1, Pst1 and Not1; followed by gel check, extraction and purification
· Restriction of c(pFadBA) by EcoR1 and Pst1
· Ran 0.8% gel for pEGFP-N1
· Ran 2% gel for BBa_K817002 (pFadBA)
June 30
Dry lab
Experiment planning and protocols revision for next week work
June 24
Wet lab
· Inoculation of pBlueScript KS(+) for training
· Autoclave basic materials
· Preparing LB
· LB-Ampicillin plates poured Dry lab
Protocols review
June 25
Wet lab
· Plasmid extraction of pBlueScript KS(+)
· LB-Chloramphenicol plates poured
Dry lab
· Primers design for FABP1, PPARa, GRP78
· Informal meeting
June 26
Wet lab
· Extraction of gDNA from HepG2 Cells for FABP1 and PPARa
· Transformation of pEGFP-N1
· Transformation of BBa_K817002 (pFadBA)
June 27
Wet lab
· Inoculation of pEGFP-N1 for plasmid extraction
· Inoculation of BBa_K817002 (pFadBA) for plasmid extraction
June 28
Wet lab
· Extraction of pEGFP-N1 plasmids by miniprep
· Extraction of BBa_K817002 (pFadBA)
· Restriction of pEGFP-N1 Restriction digestion by Ase1 and bamH1 for PPARa; Ase1 and Xho1 for GRP78, Xba1 and BamH1 for FABP1, Pst1 and Not1; followed by gel check, extraction and purification
· Restriction of c(pFadBA) by EcoR1 and Pst1
· Ran 0.8% gel for pEGFP-N1
· Ran 2% gel for BBa_K817002 (pFadBA)
June 30
Dry lab
Experiment planning and protocols revision for next week work
